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The present study was designed to investigate roles of adrenergic signaling and the endogenous hydrogen sulfide producing enzyme cystathionine \u03b2-synthetase (CBS) in a previously validated rat model of IBS induced by neonatal colonic inflammation (NCI). Here we showed that NCI-induced visceral hypersensitivity (VH) was significantly attenuated by \u03b2<jats:sub>2<\/jats:sub> subunit inhibitor but not by \u03b2<jats:sub>1<\/jats:sub> or \u03b2<jats:sub>3<\/jats:sub> or \u03b1 subunit inhibitor. NCI markedly elevated plasma norepinephrine (NE) concentration without alteration in expression of \u03b2<jats:sub>2<\/jats:sub> subunit receptors in dorsal root ganglion (DRGs) innervating the colon. In addition, NCI markedly enhanced TRPV1 and CBS expression in the colon DRGs. CBS inhibitor AOAA reversed the upregulation of TRPV1 in NCI rats. <jats:italic>In vitro<\/jats:italic> experiments showed that incubation of DRG cells with NE markedly enhanced expression of TRPV1, which was reversed by application of AOAA. Incubation of DRG cells with the H<jats:sub>2<\/jats:sub>S donor NaHS greatly enhanced TRPV1 expression. Collectively, these data suggest that activation of adrenergic signaling by NCI sensitizes TRPV1 channel activity, which is likely mediated by upregulation of CBS expression in peripheral sensory neurons, thus contributing to chronic visceral hypersensitivity.<\/jats:p>","DOI":"10.1038\/srep16109","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T11:50:35Z","timestamp":1446551435000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Adrenergic stimulation sensitizes TRPV1 through upregulation of cystathionine \u03b2-synthetase in a rat model of visceral hypersensitivity"],"prefix":"10.1038","volume":"5","author":[{"given":"Liyan","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruobing","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Yan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongmeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Yin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,11,3]]},"reference":[{"key":"BFsrep16109_CR1","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1016\/0016-5085(84)90706-6","volume":"87","author":"RS Sandler","year":"1984","unstructured":"Sandler, R. 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