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Combination treatments help to combat resistance. Here we develop a novel, effective target for combination antifungal therapy. Different aminoglycoside antibiotics combined with different sulphate-transport inhibitors produced strong, synergistic growth-inhibition of several fungi. Combinations decreased the respective MICs by \u22658-fold. Synergy was suppressed in yeast mutants resistant to effects of sulphate-mimetics (like chromate or molybdate) on sulphate transport. By different mechanisms, aminoglycosides and inhibition of sulphate transport cause errors in mRNA translation. The mistranslation rate was stimulated up to 10-fold when the agents were used in combination, consistent with this being the mode of synergistic action. A range of undesirable fungi were susceptible to synergistic inhibition by the combinations, including the human pathogens <jats:italic>Candida albicans, C. glabrata<\/jats:italic> and <jats:italic>Cryptococcus neoformans,<\/jats:italic> the food spoilage organism <jats:italic>Zygosaccharomyces bailii<\/jats:italic> and the phytopathogens <jats:italic>Rhizoctonia solani<\/jats:italic> and <jats:italic>Zymoseptoria tritici.<\/jats:italic> There was some specificity as certain fungi were unaffected. There was no synergy against bacterial or mammalian cells. The results indicate that translation fidelity is a promising new target for combinatorial treatment of undesirable fungi, the combinations requiring substantially decreased doses of active components compared to each agent alone.<\/jats:p>","DOI":"10.1038\/srep16700","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T10:46:30Z","timestamp":1447757190000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Novel, Synergistic Antifungal Combinations that Target Translation Fidelity"],"prefix":"10.1038","volume":"5","author":[{"given":"Elena","family":"Moreno-Martinez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy","family":"Vallieres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara L.","family":"Holland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon V.","family":"Avery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,11,17]]},"reference":[{"key":"BFsrep16700_CR1","doi-asserted-by":"publisher","first-page":"791","DOI":"10.2217\/fmb.14.32","volume":"9","author":"R Calderone","year":"2014","unstructured":"Calderone, R. et al. 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