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The UPT-LF POCT assay was established to quantitatively detect <jats:italic>F. tularensis<\/jats:italic> within 15\u2009min and the sensitivity of the assay was 10<jats:sup>4<\/jats:sup>\u2009CFU\u2009\u00b7\u2009mL<jats:sup>\u22121<\/jats:sup> (100\u2009CFU\/test). The linear quantitative range covered five orders of magnitude and the coefficients of variation were less than 10%. Except <jats:italic>Shigella dysenteriae<\/jats:italic>, UPT-LF showed excellent specificity to four strains that are also potential biowarfare\/bioterrorism agents and 13 food-borne pathogenic strains. Samples with pH 2\u201313, high ion strengths (\u22652\u2009mol\u2009\u00b7\u2009L<jats:sup>\u22121<\/jats:sup> solution of KCl and NaCl), high viscosities (\u226450\u2009mg\u2009\u00b7\u2009mL<jats:sup>\u22121<\/jats:sup> PEG20000 or \u226520% glycerol) and high concentrations of biomacromolecules (\u2265400\u2009mg\u2009\u00b7\u2009mL<jats:sup>\u22121<\/jats:sup> bovine serum albumin or \u226580\u2009mg\u2009\u00b7\u2009mL<jats:sup>\u22121<\/jats:sup> casein) showed little influence on the assay. For practical utilization, the tolerance limits for seven powders and eight viscera were determined and operation errors of liquid measurement demonstrated a minor influence on the strip. Ftu-UPT-LF is a candidate POCT method because of its excellent sensitivity, specificity, and stability in complex samples, as well as low operation error.<\/jats:p>","DOI":"10.1038\/srep17178","type":"journal-article","created":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T11:25:49Z","timestamp":1448537149000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid detection of Francisella tularensis"],"prefix":"10.1038","volume":"5","author":[{"given":"Fei","family":"Hua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongyun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,11,26]]},"reference":[{"key":"BFsrep17178_CR1","doi-asserted-by":"publisher","first-page":"2353","DOI":"10.1007\/s10096-012-1576-z","volume":"31","author":"H Simsek","year":"2012","unstructured":"Simsek, H., Taner, M., Karadenizli, A., Ertek, M. & Vahaboglu, H. 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