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The point mutation at codon 198 (GAG\u2009\u2192\u2009GCG, E198A) occurs in more than 90% of field resistant populations in China. Traditional detection methods of benzimidazole-resistant mutants of <jats:italic>S. sclerotiorum<\/jats:italic> are time-consuming, tedious and inefficient. To establish a suitable and rapid detection of benzimidazole-resistant mutants of <jats:italic>S. sclerotiorum<\/jats:italic>, an efficient and simple method with high specificity was developed based on loop-mediated isothermal amplification (LAMP). Eight sets of LAMP primers were designed and four sets were optimized to specially distinguish benzimidazole-resistant mutants of <jats:italic>S. sclerotiorum<\/jats:italic>. With the optimal LAMP primers, the concentration of LAMP components was optimized and the reaction conditions were set as 60\u201364\u2009\u00b0C for 60\u2009min. This method had a good specificity, sensitivity, stability and repeatability. In the 1491 sclerotia, 614 (41.18%) were positive by LAMP and 629 (42.19%) positive by MIC. Therefore, the LAMP assay is more feasible to detect benzimidazole-resistant mutants of <jats:italic>S. sclerotiorum<\/jats:italic> than traditional detection methods.<\/jats:p>","DOI":"10.1038\/srep17278","type":"journal-article","created":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T10:21:19Z","timestamp":1448533279000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Development and application of loop-mediated isothermal amplification for detecting the highly benzimidazole-resistant isolates in Sclerotinia sclerotiorum"],"prefix":"10.1038","volume":"5","author":[{"given":"Ya Bing","family":"Duan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong Chao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling Ling","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming Guo","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,11,26]]},"reference":[{"key":"BFsrep17278_CR1","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1080\/07060669409500766","volume":"16","author":"GJ Boland","year":"1994","unstructured":"Boland, G. 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