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Here we report a label-free PL genosensor for sensitive detection of <jats:italic>Vibrio cholerae<\/jats:italic> that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size &gt;30\u2009nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of <jats:italic>V. cholerae<\/jats:italic> was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700\u2009nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500\u2009ng\/\u03bcL with a sensitivity of 1.306\u2009emi\/ng, detection limit of 3.133\u2009ng\/\u03bcL and a regression coefficient (R<jats:sup>2<\/jats:sup>) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.<\/jats:p>","DOI":"10.1038\/srep17384","type":"journal-article","created":{"date-parts":[[2015,11,27]],"date-time":"2015-11-27T11:10:06Z","timestamp":1448622606000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection"],"prefix":"10.1038","volume":"5","author":[{"given":"Manoj Kumar","family":"Patel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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