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We performed mutational analyses of <jats:italic>PKD<\/jats:italic> genes in 49 unrelated patients using direct PCR-sequencing and multiplex ligation-dependent probe amplification (MLPA) for <jats:italic>PKD1<\/jats:italic> and <jats:italic>PKD2<\/jats:italic>. RT-PCR analysis was also performed in a family with a novel <jats:italic>PKD2<\/jats:italic> splicing mutation. Disease-causing mutations were identified in 44 (89.8%) of the patients: 42 (95.5%) of the patients showed mutations in <jats:italic>PKD1<\/jats:italic> and 2 (4.5%) showed mutations in <jats:italic>PKD2<\/jats:italic>. Ten nonsense, 17 frameshift, 4 splicing and one in-frame mutation were found in 32 of the patients. Large rearrangements were found in 3 patients and missense mutations were found in 9 patients. Approximately 61.4% (27\/44) of the mutations are first reported with a known mutation rate of 38.6%. RNA analysis of a novel <jats:italic>PKD2<\/jats:italic> mutation (c.595_595\u2009+\u200914delGGTAAGAGCGCGCGA) suggested monoallelic expression of the wild-type allele. Furthermore, patients with <jats:italic>PKD1<\/jats:italic>-truncating mutations reached end-stage renal disease (ESRD) earlier than patients with non-truncating mutations (47\u2009\u00b1\u20093.522 years vs. 59\u2009\u00b1\u200911.687 years, <jats:italic>P<\/jats:italic>\u2009=\u20090.016). The mutation screening of <jats:italic>PKD<\/jats:italic> genes in Chinese ADPKD patients will enrich our mutation database and significantly contribute to improve genetic counselling for ADPKD patients.<\/jats:p>","DOI":"10.1038\/srep17468","type":"journal-article","created":{"date-parts":[[2015,12,3]],"date-time":"2015-12-03T10:47:52Z","timestamp":1449139672000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Identification of novel PKD1 and PKD2 mutations in a Chinese population with autosomal dominant polycystic kidney disease"],"prefix":"10.1038","volume":"5","author":[{"given":"Bei","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song-Chang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Mei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye-Qing","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ting","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Yue","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Feng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Ming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,3]]},"reference":[{"key":"BFsrep17468_CR1","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1038\/ncpneph0070","volume":"2","author":"VE Torres","year":"2006","unstructured":"Torres, V. 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