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The high rate of antigenic drift (viral mutation) and the putative antigenic shift (reassortant strains) have raised the need to find the host cell inducible factors modulating IAV replication and its pathogenesis to develop more effective antiviral treatment. In this study, we found for the first time that transcription factor Runx3, a developmental regulator and tumor suppressor, was induced by IAV H1N1 and H3N2, viral RNA, a synthetic analog of viral double-stranded RNA (dsRNA) polyinosinic-polycytidylic acid and type-II interferon-\u03b3 (IFN\u03b3) in human airway epithelial cells. Whereas Runx3 was essentially not induced by type-I IFN\u03b1 and type-III IFN\u03bb, we show that Runx3 induction by IAV infection and viral RNA is mediated through the innate immune receptor MDA5 and the I\u03baB kinase-\u03b2\u2212NF-\u03baB pathway. Moreover, we provide substantial evidence indicating that Runx3 plays a crucial role in airway epithelial cell apoptosis induced by IAV infection and dsRNA through the activation of extrinsic and intrinsic apoptosis pathways. Thus, we have identified Runx3 as an inducible and important transcription factor modulating IAV-induced host epithelial cell apoptosis.<\/jats:p>","DOI":"10.1038\/srep17916","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T10:31:43Z","timestamp":1449570703000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Transcription Factor Runx3 Is Induced by Influenza A Virus and Double-Strand RNA and Mediates Airway Epithelial Cell Apoptosis"],"prefix":"10.1038","volume":"5","author":[{"given":"Huachen","family":"Gan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Idell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,8]]},"reference":[{"key":"BFsrep17916_CR1","doi-asserted-by":"publisher","first-page":"2397","DOI":"10.1016\/j.ijcard.2013.01.274","volume":"167","author":"ZR Estabragh","year":"2013","unstructured":"Estabragh, Z. 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