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Current work investigated its <jats:italic>in vitro<\/jats:italic> anti-tumor activity and underlying mechanism on HepG2 cells. Akbu-LAAO inhibited HepG2 growth time and dose-dependently with an IC<jats:sub>50<\/jats:sub> of ~38.82\u2009\u03bcg\/mL. It could induce the apoptosis of HepG2 cells. Akbu-LAAO exhibited cytotoxicity by inhibiting growth and inducing apoptosis of HepG2 as it showed no effect on its cell cycle. The inhibition of Akbu-LAAO to HepG2 growth partially relied on enzymatic-released H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> as catalase only partially antagonized this effect. cDNA microarray results indicated TGF-\u03b2 signaling pathway was linked to the cytotoxicity of Akbu-LAAO on HepG2. TGF-\u03b2 pathway related molecules <jats:italic>CYR61, p53, GDF15, TOB1, BTG2, BMP2, BMP6, SMAD9, JUN, JUNB, LOX, CCND1, CDK6, GADD45A, CDKN1A<\/jats:italic> were deregulated in HepG2 following Akbu-LAAO stimulation. The presence of catalase only slightly restored the mRNA changes induced by Akbu-LAAO for differentially expressed genes. Meanwhile, LDN-193189, a TGF-\u03b2 pathway inhibitor reduced Akbu-LAAO cytotoxicity on HepG2. Collectively, we reported, for the first time, SV-LAAO showed anti-tumor cell activity <jats:italic>via<\/jats:italic> TGF-\u03b2 pathway. It provides new insight of SV-LAAO exhibiting anti-tumor effect <jats:italic>via<\/jats:italic> a novel signaling pathway.<\/jats:p>","DOI":"10.1038\/srep18215","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T11:38:26Z","timestamp":1450093106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Akbu-LAAO exhibits potent anti-tumor activity to HepG2 cells partially through produced H2O2 via TGF-\u03b2 signal pathway"],"prefix":"10.1038","volume":"5","author":[{"given":"Chunmei","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panpan","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongting","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Tao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Zhong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,12,14]]},"reference":[{"key":"BFsrep18215_CR1","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.fsi.2014.09.017","volume":"41","author":"R Li","year":"2014","unstructured":"Li, R. & Li, A. 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