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This work describes the developmental validation of an X-InDels panel amplifying 18 bi-allelic markers and Amelogenin in one single PCR system. Developmental validation indicated that this novel panel was reproducible, accurate, sensitive and robust for forensic application. Sensitivity testing of the panel was such that a full profile was obtainable even with 125\u2009pg of human DNA with intra-locus balance above 70%. Specificity testing was demonstrated by the lack of cross-reactivity with a variety of commonly encountered animal species and microorganisms. For the stability testing in cases of PCR inhibition, full profiles have been obtained with hematin (\u22641000\u2009\u03bcM) and humic acid (\u2264150\u2009ng\/\u03bcL). For the forensic investigation of the 18 X-InDels in the HAN population of China, no locus deviated from the Hardy\u2013Weinberg equilibrium and linkage disequilibrium. Since they are independent from each other, the CDP<jats:sub>female<\/jats:sub> was 0.999999726 and CDP<jats:sub>male<\/jats:sub> was 0.999934223. The forensic parameters suggested that this X-Indel panel is polymorphic and informative, which provides valuable X-linked information for deficient relationship cases where autosomal markers are uninformative.<\/jats:p>","DOI":"10.1038\/srep18336","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T10:35:50Z","timestamp":1450089350000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Developmental validation of an X-Insertion\/Deletion polymorphism panel and application in HAN population of China"],"prefix":"10.1038","volume":"5","author":[{"given":"Suhua","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingnan","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoneng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengtao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,14]]},"reference":[{"key":"BFsrep18336_CR1","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1002\/(SICI)1522-2683(19990101)20:8<1682::AID-ELPS1682>3.0.CO;2-Z","volume":"20","author":"R Chakraborty","year":"1999","unstructured":"Chakraborty, R., Stivers, D. 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