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Ectopic lipids (intramyocellular (IMCL) and intrahepatocellular lipids (IHCL) are related to insulin resistance. The effect of GHRT on ectopic lipids is unknown. It is hypothesized that exercise-induced utilization of ectopic lipids is significantly decreased in GHD patients and normalized by GHRT. GHD (4 females, 6 males) and age\/gender\/waist-matched control subjects (CS) were studied. VO<jats:sub>2max<\/jats:sub> was assessed on a treadmill and insulin sensitivity determined by a two-step hyperinsulinaemic-euglycaemic clamp. Visceral (VAT) and subcutaneous (SAT) fat were quantified by MR-imaging. IHCL and IMCL were measured before and after a 2\u2009h exercise at 50\u201360% of VO<jats:sub>2max<\/jats:sub> using MR-spectroscopy (\u2206IMCL, \u2206IHCL). Identical investigations were performed after 6 months of GHRT. VO<jats:sub>2max<\/jats:sub> was similar in GHD and CS and significantly increased after GHRT; GHRT significantly decreased SAT and VAT. 2\u2009h-exercise resulted in a decrease in IMCL (significant in CS and GHRT) and a significant increase in IHCL in CS and GHD pre and post GHRT. GHRT didn\u2019t significantly impact on \u2206IMCL and \u2206IHCL. We conclude that aerobic exercise affects ectopic lipids in patients and controls. 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