{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:14:51Z","timestamp":1787159691664,"version":"build-2736575974"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nucleic acid amplification tests have become a common method for diagnosis of STIs due to their improved sensitivity over immunoassays and traditional culture-based methods. Isothermal nucleic acid amplification methods offer significant advantages over polymerase chain reaction (PCR) because they do not require sophisticated instruments needed for thermal cycling of PCR. We recently reported a novel isothermal nucleic acid amplification method, Strand Invasion-Based Amplification (SIBA), which exhibited high analytical sensitivity and specificity for amplification of DNA. However, because the reactions were detected using an intercalating dye, this method was only suitable for amplifying a single genomic target. Here, we report the development of multiplexed SIBA (mSIBA) that allows simultaneous detection of\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    (CT),\n                    <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    (NG) and an internal control in the same reaction tube. SIBA is compatible with probes, allowing the detection of multiple DNA targets in the same reaction tube. The IC was developed to assess the quality of the isolated DNA and the integrity of the enzyme system, as well as to test oligonucleotides. The mSIBA assay retained high analytical sensitivity and specificity for the detection of CT and NG. The development of mSIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.\n                  <\/jats:p>","DOI":"10.1038\/srep20487","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T06:06:38Z","timestamp":1454479598000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Multiplex Strand Invasion Based Amplification (mSIBA) assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae"],"prefix":"10.1038","volume":"6","author":[{"given":"Kevin E.","family":"Eboigbodin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Hoser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,3]]},"reference":[{"key":"BFsrep20487_CR1","unstructured":"Centers for Disease Control and Prevention. Sexually Transmitted Disease Surveillance 2013. Atlanta: U.S. Department of Health and Human Services (2014)."},{"key":"BFsrep20487_CR2","doi-asserted-by":"publisher","first-page":"e29","DOI":"10.1093\/nar\/29.5.e29","volume":"29","author":"LR Baugh","year":"2001","unstructured":"Baugh, L. R., Hill, A. A., Brown, E. L. & Hunter, C. P. Quantitative analysis of mRNA amplification by in vitro transcription. Nucleic. Acids. Res 29, e29 (2001).","journal-title":"Nucleic. Acids. Res"},{"key":"BFsrep20487_CR3","unstructured":"Papp, J. R., Schachter, J., Gaydos, C. A. & Van Der Pol, B. Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae \u2014 2014. MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports\/Centers for Disease Control63, 1\u201319 (2014)."},{"key":"BFsrep20487_CR4","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1128\/JCM.39.5.1751-1756.2001","volume":"39","author":"E Van Dyck","year":"2001","unstructured":"Van Dyck, E., Ieven, M., Pattyn, S., Van Damme, L. & Laga, M. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture and Three Nucleic Acid Amplification Tests. J. Clin. Microbiol. 39, 1751\u20131756 (2001).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep20487_CR5","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1128\/JCM.41.1.304-309.2003","volume":"41","author":"C Gaydos","year":"2003","unstructured":"Gaydos, C. et al. Performance of the APTIMA Combo 2 Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Female Urine and Endocervical Swab Specimens. J. Clin. Microbiol. 41, 304\u2013309 (2003).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep20487_CR6","doi-asserted-by":"publisher","first-page":"e112656","DOI":"10.1371\/journal.pone.0112656","volume":"9","author":"MJ Hoser","year":"2014","unstructured":"Hoser, M. J., Mansukoski, H. K., Morrical, S. W. & Eboigbodin, K. E. Strand Invasion Based Amplification (SIBA\u00ae): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte. PLoS ONE 9, e112656 (2014).","journal-title":"PLoS ONE"},{"key":"BFsrep20487_CR7","doi-asserted-by":"publisher","first-page":"2163","DOI":"10.1128\/JCM.00314-14","volume":"52","author":"T Edwards","year":"2014","unstructured":"Edwards, T., Burke, P., Smalley, H. B., Gillies, L. & Hobbs, G. Loop-Mediated Isothermal Amplification (LAMP) Test for the Detection of Neisseria gonorrhoeae in Urine Samples and the Tolerance of the Assay to the Presence of Urea. J. Clin. Microbiol. 52, 2163\u20132165 (2014).","journal-title":"J. Clin. Microbiol."},{"key":"BFsrep20487_CR8","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1136\/sti.2009.040634","volume":"86","author":"MJ Hopkins","year":"2010","unstructured":"Hopkins, M. J., Ashton, L. J., Alloba, F., Alawattegama, A. & Hart, I. J. Validation of a laboratory-developed real-time PCR protocol for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine. Sex Transm Infect 86, 207\u2013211 (2010).","journal-title":"Sex Transm Infect"},{"key":"BFsrep20487_CR9","doi-asserted-by":"publisher","first-page":"3607","DOI":"10.1016\/S0040-4020(98)00094-5","volume":"54","author":"A Koshkin","year":"1998","unstructured":"Koshkin, A. et al. LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation and unprecedented nucleic acid recognition. Tetrahedron 54, 3607\u20133630 (1998).","journal-title":"Tetrahedron"},{"key":"BFsrep20487_CR10","doi-asserted-by":"publisher","first-page":"4030","DOI":"10.1093\/nar\/gkm358","volume":"35","author":"C Yang","year":"2007","unstructured":"Yang, C. et al. Synthesis and investigation of deoxyribonucleic acid\/locked nucleic acid chimeric molecular beacons. Nucleic. Acids. Res 35, 4030\u20134041 (2007).","journal-title":"Nucleic. Acids. Res"},{"key":"BFsrep20487_CR11","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1128\/JCM.44.1.206-213.2006","volume":"44","author":"H Jalal","year":"2006","unstructured":"Jalal, H. et al. Development and Validation of a Rotor-Gene Real-Time PCR Assay for Detection, Identification and Quantification of Chlamydia trachomatis in a Single Reaction. J Clin Microbiol 44, 206\u2013213 (2006).","journal-title":"J Clin Microbiol"},{"key":"BFsrep20487_CR12","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/clinchem\/45.6.777","volume":"45","author":"M Little","year":"1999","unstructured":"Little, M. et al. Strand Displacement Amplification and Homogeneous Real-Time Detection Incorporated in a Second-Generation DNA Probe System, BDProbeTecET. Clin Chem 45, 777\u2013784 (1999).","journal-title":"Clin Chem"},{"key":"BFsrep20487_CR13","doi-asserted-by":"publisher","first-page":"1294","DOI":"10.1128\/JCM.02595-10","volume":"49","author":"A Cheng","year":"2011","unstructured":"Cheng, A., Qian, Q. & Kirby, J. E. Evaluation of the Abbott RealTime CT\/NG Assay in Comparison to the Roche Cobas Amplicor CT\/NG Assay. J. Clin. Microbiol. 49, 1294\u20131300 (2011).","journal-title":"J. Clin. 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Novel Bioluminescent Quantitative Detection of Nucleic Acid Amplification in Real-Time. PLoS ONE 5, e14155 (2010).","journal-title":"PLoS ONE"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep20487.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep20487","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep20487.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T21:53:52Z","timestamp":1672869232000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep20487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,3]]},"references-count":19,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,4,22]]}},"alternative-id":["BFsrep20487"],"URL":"https:\/\/doi.org\/10.1038\/srep20487","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,3]]},"assertion":[{"value":"30 July 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 January 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"KEE is an employee of Orion Diagnostica Oy. Geneform Technologies, UK is owned by Orion Diagnostica Oy. Orion Diagnostica Oy has a commercial interest in disclosure of the SIBA technology. All SIBA patents\/patent applications are owned by Orion Diagnostica Oy.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"20487"}}