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Mlp37, the closest homolog of the previously identified amino acid chemoreceptor Mlp24, was found to mediate taxis to taurine as well as <jats:sc>L<\/jats:sc>-serine, <jats:sc>L<\/jats:sc>-alanine, <jats:sc>L<\/jats:sc>-arginine, and other amino acids. Methylation of Mlp37 was enhanced upon the addition of taurine and amino acids. Isothermal titration calorimetry demonstrated that a purified periplasmic fragment of Mlp37 binds directly to taurine, <jats:sc>L<\/jats:sc>-serine, <jats:sc>L<\/jats:sc>-alanine and <jats:sc>L<\/jats:sc>-arginine. Crystal structures of the periplamic domain of Mlp37 revealed that <jats:sc>L<\/jats:sc>-serine and taurine bind to the membrane-distal PAS domain in essentially in the same way. The structural information was supported by characterising the <jats:italic>in vivo<\/jats:italic> properties of alanine-substituted mutant forms of Mlp37. The fact that the ligand-binding domain of the <jats:sc>L<\/jats:sc>-serine complex had a small opening, which would accommodate a larger R group, accounts for the broad ligand specificity of Mlp37 and allowed us to visualise ligand binding to Mlp37 with fluorescently labelled <jats:sc>L<\/jats:sc>-serine. Taken together, we conclude that Mlp37 serves as the major chemoreceptor for taurine and various amino acids.<\/jats:p>","DOI":"10.1038\/srep20866","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T10:46:22Z","timestamp":1455619582000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants"],"prefix":"10.1038","volume":"6","author":[{"given":"So-ichiro","family":"Nishiyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuaki","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumasa","family":"Sumita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michio","family":"Homma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Imada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuro","family":"Kawagishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,16]]},"reference":[{"key":"BFsrep20866_CR1","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.femsre.2004.09.003","volume":"29","author":"M Begley","year":"2005","unstructured":"Begley, M., Gahan, C. 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