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Due to the spread of multidrug-resistant strains, developing an effective vaccine is the most promising method for combating <jats:italic>S. aureus<\/jats:italic> infection. In this study, based on the immune-dominant areas of the iron surface determinant B (IsdB) and clumping factor A (ClfA), we designed the novel chimeric vaccine IsdB<jats:sub>151-277<\/jats:sub>ClfA<jats:sub>33-213<\/jats:sub> (IC). IC formulated with the AlPO<jats:sub>4<\/jats:sub> adjuvant induced higher protection in an <jats:italic>S. aureus<\/jats:italic> sepsis model compared with the single components alone and showed broad immune protection against several clinical <jats:italic>S. aureus<\/jats:italic> isolates. Immunisation with IC induced strong antibody responses. The protective effect of antibodies was demonstrated through the opsonophagocytic assay (OPA) and passive immunisation experiment. Moreover, this new chimeric vaccine induced Th1\/Th17-skewed cellular immune responses based on cytokine profiles and CD4<jats:sup>+<\/jats:sup> T cell stimulation tests. Neutralisation of IL-17A alone (but not IFN-\u03b3) resulted in a significant decrease in vaccine immune protection. Finally, we found that IC showed protective efficacy in a pneumonia model. Taken together, these data provide evidence that IC is a potentially promising vaccine candidate for combating <jats:italic>S. aureus<\/jats:italic> sepsis and pneumonia.<\/jats:p>","DOI":"10.1038\/srep20929","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T11:18:10Z","timestamp":1455189490000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Protective efficacy of the chimeric Staphylococcus aureus vaccine candidate IC in sepsis and pneumonia models"],"prefix":"10.1038","volume":"6","author":[{"given":"Liuyang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changzhi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianfei","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijie","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiming","family":"Jing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanming","family":"Zou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,11]]},"reference":[{"key":"BFsrep20929_CR1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1056\/NEJM199808203390806","volume":"339","author":"FD Lowy","year":"1998","unstructured":"Lowy, F. 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