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Intimate interaction with the cell membrane is invoked. We designed Neutron Reflectometry experiments to reveal the existence and extent of the interaction between \u03b2\u2013amyloid (A\u03b2) peptides and a lone customized biomimetic membrane and their dependence on the aggregation state of the peptide. The membrane, asymmetrically containing phospholipids, GM1 and cholesterol in biosimilar proportion, is a model for a raft, a putative site for amyloid-cell membrane interaction. We found that the structured-oligomer of A\u03b2(1-42), its most acknowledged membrane-active state, is embedded as such into the external leaflet of the membrane. Conversely, the A\u03b2(1-42) unstructured early-oligomers deeply penetrate the membrane, likely mimicking the interaction at neuronal cell surfaces, when the A\u03b2(1-42) is cleaved from APP protein and the membrane constitutes a template for its further structural evolution. Moreover, the smaller A\u03b2(1-6) fragment, the N-terminal portion of A\u03b2, was also used. A\u03b2 N-terminal is usually considered as involved in oligomer stabilization but not in the peptide-membrane interaction. Instead, it was seen to remove lipids from the bilayer, thus suggesting its role, once in the whole peptide, in membrane leakage, favouring peptide recruitment.<\/jats:p>","DOI":"10.1038\/srep20997","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T10:50:12Z","timestamp":1455619812000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Amyloid\u03b2 Peptides in interaction with raft-mime model membranes: a neutron reflectivity insight"],"prefix":"10.1038","volume":"6","author":[{"given":"Valeria","family":"Rondelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Brocca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Motta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Messa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Colombo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Salmona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Fragneto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Cant\u00f9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Del Favero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,16]]},"reference":[{"key":"BFsrep20997_CR1","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1111\/j.1471-4159.2006.04212.x","volume":"99","author":"DH Small","year":"2006","unstructured":"Small, D. 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