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In this study, we have provided evidences for the smoking-induced hemolysis using two methods: spectra of blood components and atomic force microscopic analysis of surface morphology. A total of 62 subjects (control\u2009=\u200931; smoker\u2009=\u200931: 21 male; 10 female in each set) were considered for the study. The findings indicate that smoking leads to potholes on the surface, swelling of shape, rupturing of erythrocytes, removal of hematoporphyrin and flushing into the plasma as metabolites of the erythrocyte. The overall morphology of the erythrocytes of the smoker group appears more like a Mexican hat. The mean surface roughness was 5.5\u2009\u00b1\u20093\u2009nm for the smoker group, but 1.2\u2009\u00b1\u20090.2\u2009nm for the control group. Such damages might help the toxins, (CO, peroxidants, aldehydes etc.,) to gain easy access and get strongly absorbed by the hemoglobin, leading to enhanced rates of hemolysis as shown by the spectral features of metabolites. This indicates that the average life span of the smoker\u2019s erythrocytes is significantly less than that of the control group.<\/jats:p>","DOI":"10.1038\/srep21095","type":"journal-article","created":{"date-parts":[[2016,2,19]],"date-time":"2016-02-19T10:18:59Z","timestamp":1455877139000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Smoking Induced Hemolysis: Spectral and microscopic investigations"],"prefix":"10.1038","volume":"6","author":[{"given":"Vadivel","family":"Masilamani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid","family":"AlZahrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhanasamy","family":"Devanesan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadi","family":"AlQahtani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Saleh","family":"AlSalhi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,2,19]]},"reference":[{"key":"BFsrep21095_CR1","first-page":"116","volume":"10","author":"S Asgary","year":"2005","unstructured":"Asgary, S. 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