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However, yfdX protein along with other proteins involved in acid tolerance response is reported to be up regulated by the multidrug response regulatory system in <jats:italic>E. coli<\/jats:italic>. Hence, molecular and functional characterization of this protein is important for understanding of key cellular processes in bacterial cells. Here we study STY3178, a yfdX protein from a MDR strain of typhoid fever causing <jats:italic>Salmonella<\/jats:italic> Typhi. Our experimental results indicate that STY3178 is a helical protein existing in a trimeric oligomerization state in solution. We also observe many small antibiotics, like ciprofloxacin, rifampin and ampicillin viably interact with this protein. The dissociation constants from the quenching of steady state fluorescence and isothermal titration calorimetry show that ciprofloxacin binding is stronger than rifampin followed by ampicillin.<\/jats:p>","DOI":"10.1038\/srep21305","type":"journal-article","created":{"date-parts":[[2016,2,19]],"date-time":"2016-02-19T10:06:45Z","timestamp":1455876405000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi"],"prefix":"10.1038","volume":"6","author":[{"given":"Paramita","family":"Saha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camelia","family":"Manna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santasabuj","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahua","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,2,19]]},"reference":[{"key":"BFsrep21305_CR1","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1107\/S1744309110001685","volume":"66","author":"A Bateman","year":"2010","unstructured":"Bateman A., Coggill P. & Finn R. 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