{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:30:57Z","timestamp":1790483457948,"version":"4.1.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T00:00:00Z","timestamp":1456099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T00:00:00Z","timestamp":1456099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Identification of biologically active natural compounds that promote health and longevity and understanding how they act, will provide insights into aging and metabolism and strategies for developing agents that prevent chronic disease. The garlic-derived thioallyl compounds <jats:italic>S<\/jats:italic>-allylcysteine (SAC) and <jats:italic>S<\/jats:italic>-allylmercaptocysteine (SAMC) have been shown to have multiple biological activities. Here we show that SAC and SAMC increase lifespan and stress resistance in <jats:italic>Caenorhabditis elegans<\/jats:italic> and reduce accumulation of reactive oxygen species (ROS). These compounds do not appear to activate DAF-16 (FOXO orthologue) or mimic dietary restriction (DR) effects, but selectively induce SKN-1 (Nrf1\/2\/3 orthologue) targets involved in oxidative stress defense. Interestingly, their treatments do not facilitate SKN-1 nuclear accumulation, but slightly increased intracellular SKN-1 levels. Our data also indicate that thioallyl structure and the number of sulfur atoms are important for SKN-1 target induction. Our results indicate that SAC and SAMC may serve as potential agents that slow aging.<\/jats:p>","DOI":"10.1038\/srep21611","type":"journal-article","created":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T10:34:56Z","timestamp":1456137296000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Natural thioallyl compounds increase oxidative stress resistance and lifespan in Caenorhabditis elegans by modulating SKN-1\/Nrf"],"prefix":"10.1038","volume":"6","author":[{"given":"Takahiro","family":"Ogawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Kodera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dai","family":"Hirata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Keith","family":"Blackwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Mizunuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,2,22]]},"reference":[{"key":"BFsrep21611_CR1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1749-6632.1999.tb07814.x","volume":"893","author":"I Fridovich","year":"1999","unstructured":"Fridovich, I. Fundamental aspects of reactive oxygen species, or what\u2019s the matter with oxygen? Ann. N. Y. Acad. Sci. 893, 13\u201318 (1999).","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"BFsrep21611_CR2","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/0005-2760(81)90049-7","volume":"664","author":"HW Leung","year":"1981","unstructured":"Leung, H. W., Vang, M. J. & Mavis, R. D. The cooperative interaction between vitamin E and vitamin C in suppression of peroxidation of membrane phospholipids. Biochim. Biophys. Acta. 664, 266\u2013272 (1981).","journal-title":"Biochim. Biophys. Acta."},{"key":"BFsrep21611_CR3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.etp.2010.11.005","volume":"64","author":"CE Guerrero-Beltr\u00e1n","year":"2012","unstructured":"Guerrero-Beltr\u00e1n, C. E., Calder\u00f3n-Oliver, M., Pedraza-Chaverri, J. & Chirino, Y. I. Protective effect of sulforaphane against oxidative stress: recent advances. Exp. Toxicol. Pathol. 64, 503\u2013508 (2012).","journal-title":"Exp. Toxicol. Pathol."},{"key":"BFsrep21611_CR4","first-page":"133","volume":"4","author":"H Sumiyoshi","year":"1989","unstructured":"Sumiyoshi, H. & Wargovich, M. J. Garlic (Allium sativum): a review of its relationship to cancer. Asia Pacific J. Pharmacol. 4, 133\u2013140 (1989).","journal-title":"Asia Pacific J. Pharmacol."},{"key":"BFsrep21611_CR5","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/S0140-6736(79)91239-X","volume":"1","author":"AN Makheja","year":"1979","unstructured":"Makheja, A. N., Vanderhoek, J. Y. & Bailey, J. M. Inhibition of platelet aggregation and thromboxane synthesis by onion and garlic. Lancet 1, 781 (1979).","journal-title":"Lancet"},{"key":"BFsrep21611_CR6","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1007\/BF02535329","volume":"17","author":"VS Kamanna","year":"1982","unstructured":"Kamanna, V. S. & Chandrasekhara, N. Effect of garlic (Allium sativum linn) on serum lipoproteins and lipoprotein cholesterol levels in albino rats rendered hypercholesteremic by feeding cholesterol. Lipids 17, 483\u2013488 (1982).","journal-title":"Lipids"},{"key":"BFsrep21611_CR7","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1055\/s-2007-969111","volume":"52","author":"H Hikino","year":"1986","unstructured":"Hikino, H. et al. Antihepatotoxic actions of Allium sativum bulbs. Planta Med. 52, 163\u2013168 (1986).","journal-title":"Planta Med."},{"key":"BFsrep21611_CR8","first-page":"5084","volume":"50","author":"H Sumiyoshi","year":"1990","unstructured":"Sumiyoshi, H. & Wargovich, M. J. Chemoprevention of 1,2-dimethyl hydrazine-induced colon cancer in mice by naturally occurring organo-sulfur compounds. Cancer Res. 50, 5084\u20135087 (1990).","journal-title":"Cancer Res."},{"key":"BFsrep21611_CR9","doi-asserted-by":"crossref","first-page":"H2693","DOI":"10.1152\/ajpheart.00853.2007","volume":"293","author":"SC Chuah","year":"2007","unstructured":"Chuah, S. C., Moore, P. K. & Zhu, Y. Z. S-allylcysteine mediates cardioprotection in an acute myocardial infarction rat model via a hydrogen sulfide-mediated pathway. Am. J. Physiol. Heart Circ. Physiol. 293, H2693\u2013H2701 (2007).","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"BFsrep21611_CR10","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1023\/A:1021946210399","volume":"22","author":"T Moriguchi","year":"1997","unstructured":"Moriguchi, T. et al. Neurotrophic activity of organosulfur compounds having a thioallyl group on cultured rat hippocampal neurons. Neurochem. Res. 22, 1449\u20131452 (1997).","journal-title":"Neurochem. Res."},{"key":"BFsrep21611_CR11","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.fct.2005.08.012","volume":"44","author":"CC Hsu","year":"2006","unstructured":"Hsu, C. C., Lin, C. C., Liao, T. S. & Yin, M. C. Protective effect of s-allyl cysteine and s-propyl cysteine on acetaminophen-induced hepatotoxicity in mice. Food Chem. Toxicol. 44, 393\u2013397 (2006).","journal-title":"Food Chem. Toxicol."},{"key":"BFsrep21611_CR12","first-page":"725","volume":"61","author":"H Shirin","year":"2001","unstructured":"Shirin, H. et al. Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulfide. Cancer Res. 61, 725\u2013731 (2001).","journal-title":"Cancer Res."},{"key":"BFsrep21611_CR13","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1254\/jjp.78.199","volume":"78","author":"I Sumioka","year":"1998","unstructured":"Sumioka, I., Matsura, T., Kasuga, S., Itakura, Y. & Yamada, K. Mechanisms of protection by S-allylmercaptocysteine against acetaminophen-induced liver injury in mice. Jpn. J. Pharmacol. 78, 199\u2013207 (1998).","journal-title":"Jpn. J. Pharmacol."},{"key":"BFsrep21611_CR14","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2174\/1568009033333736","volume":"3","author":"M Thomson","year":"2003","unstructured":"Thomson, M. & Ali, M. Garlic [Allium sativum]: A review of its potential use as an anti-cancer agent. Curr. Cancer Drug Targets 3, 67\u201381 (2003).","journal-title":"Curr. Cancer Drug Targets"},{"key":"BFsrep21611_CR15","first-page":"787","volume":"2","author":"G Li","year":"1995","unstructured":"Li, G. et al. Antiproliferative effects of garlic constituents in cultured human breast-cancer cells. Oncol. Rep. 2, 787\u2013791 (1995).","journal-title":"Oncol. Rep."},{"key":"BFsrep21611_CR16","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1093\/carcin\/17.5.1041","volume":"17","author":"S Hatono","year":"1996","unstructured":"Hatono, S., Jimenez, A. & Wargovich, M. J. Chemopreventive effect of S-allylcysteine and its relationship to the detoxification enzyme glutathione S-transferase. Carcinogenesis 17, 1041\u20131044 (1996).","journal-title":"Carcinogenesis"},{"key":"BFsrep21611_CR17","first-page":"3299","volume":"61","author":"M McMahon","year":"2001","unstructured":"McMahon, M. et al. The Cap\u2019n\u2019Collar basic leucine zipper transcription factor Nrf2 (NF-E2 p45-related factor 2) controls both constitutive and inducible expression of intestinal detoxification and glutathione biosynthetic enzymes. Cancer Res. 61, 3299\u20133307 (2001).","journal-title":"Cancer Res."},{"key":"BFsrep21611_CR18","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1111\/jnc.12986","volume":"133","author":"H Shi","year":"2014","unstructured":"Shi, H. et al. S-allyl cysteine activates the Nrf2-dependent antioxidant response and protects neurons against ischemic injury in vitro and in vivo. J. Neurochem. 133, 298\u2013308 (2014).","journal-title":"J. Neurochem."},{"key":"BFsrep21611_CR19","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1038\/366461a0","volume":"366","author":"C Kenyon","year":"1993","unstructured":"Kenyon, C., Chang, J., Gensch, E., Rudner, A. & Tabtiang, R. A C. elegans mutant that lives twice as long as wild type. Nature 366, 461\u2013464 (1993).","journal-title":"Nature"},{"key":"BFsrep21611_CR20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/nature14021","volume":"519","author":"CY Ewald","year":"2015","unstructured":"Ewald, C. Y., Landis, J. N., Porter Abate, J., Murphy, C. T. & Blackwell, T. K. Dauer-independent insulin\/IGF-1-signalling implicates collagen remodelling in longevity. Nature 519, 97\u2013101 (2015).","journal-title":"Nature"},{"key":"BFsrep21611_CR21","doi-asserted-by":"crossref","first-page":"re3","DOI":"10.1126\/scisignal.3112re3","volume":"3","author":"GP Sykiotis","year":"2010","unstructured":"Sykiotis, G. P. & Bohmann, D. Stress-activated cap\u2019n\u2019collar transcription factors in aging and human disease. Sci. Signal. 3, re3 (2010).","journal-title":"Sci. Signal."},{"key":"BFsrep21611_CR22","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nature01789","volume":"424","author":"CT Murphy","year":"2003","unstructured":"Murphy, C. T. et al. Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature 424, 277\u2013283 (2003).","journal-title":"Nature"},{"key":"BFsrep21611_CR23","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1126\/science.1083614","volume":"300","author":"SS Lee","year":"2003","unstructured":"Lee, S. S., Kennedy, S., Tolonen, A. C. & Ruvkun, G. DAF-16 target genes that control C. elegans life-span and metabolism. Science 300, 644\u2013647 (2003).","journal-title":"Science"},{"key":"BFsrep21611_CR24","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.cell.2008.01.030","volume":"132","author":"JMA Tullet","year":"2008","unstructured":"Tullet, J. M. A. et al. Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans. Cell 132, 1025\u20131038 (2008).","journal-title":"Cell"},{"key":"BFsrep21611_CR25","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/0092-8674(92)90078-Q","volume":"68","author":"B Bowerman","year":"1992","unstructured":"Bowerman, B., Eaton, B. A. & Priess, J. R. skn-1, a maternally expressed gene required to specify the fate of ventral blastomeres in the early C. elegans embryo. Cell 68, 1061\u20131075 (1992).","journal-title":"Cell"},{"key":"BFsrep21611_CR26","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1101\/gad.1107803","volume":"17","author":"JH An","year":"2003","unstructured":"An, J. H. & Blackwell, T. K. SKN-1 links C. elegans mesendodermal specification to a conserved oxidative stress response. Genes dev. 17, 1882\u20131893 (2003).","journal-title":"Genes dev."},{"key":"BFsrep21611_CR27","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1111\/j.1474-9726.2009.00501.x","volume":"8","author":"RP Oliveira","year":"2009","unstructured":"Oliveira, R. P. et al. Condition-adapted stress and longevity gene regulation by Caenorhabditis elegans SKN-1\/Nrf. Aging Cell 8, 524\u2013541 (2009).","journal-title":"Aging Cell"},{"key":"BFsrep21611_CR28","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.cmet.2012.04.007","volume":"15","author":"S Robida-Stubbs","year":"2012","unstructured":"Robida-Stubbs, S. et al. TOR signaling and rapamycin influence longevity by regulating SKN-1\/Nrf and DAF-16\/FoxO. Cell Metab. 15, 713\u2013724 (2012).","journal-title":"Cell Metab."},{"key":"BFsrep21611_CR29","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1111\/acel.12248","volume":"13","author":"M Mizunuma","year":"2014","unstructured":"Mizunuma, M., Neumann-Haefelin, E., Moroz, N., Li, Y. & Blackwell, T. K. mTORC2-SGK-1 acts in two environmentally responsive pathways with opposing effects on longevity. Aging Cell 13, 869\u2013878 (2014).","journal-title":"Aging Cell"},{"key":"BFsrep21611_CR30","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1101\/gad.1324805","volume":"19","author":"H Inoue","year":"2005","unstructured":"Inoue, H. et al. The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response. Genes dev. 19, 2278\u20132283 (2005).","journal-title":"Genes dev."},{"key":"BFsrep21611_CR31","doi-asserted-by":"crossref","first-page":"e1001048","DOI":"10.1371\/journal.pgen.1001048","volume":"6","author":"J Wang","year":"2010","unstructured":"Wang, J. et al. RNAi screening implicates a SKN-1-dependent transcriptional response in stress resistance and longevity deriving from translation inhibition. PLoS Genet. 6, e1001048 (2010).","journal-title":"PLoS Genet."},{"key":"BFsrep21611_CR32","doi-asserted-by":"crossref","first-page":"e1002119","DOI":"10.1371\/journal.pgen.1002119","volume":"7","author":"X Li","year":"2011","unstructured":"Li, X. et al. Specific SKN-1\/Nrf stress responses to perturbations in translation elongation and proteasome activity. PLoS Genet. 7, e1002119 (2011).","journal-title":"PLoS Genet."},{"key":"BFsrep21611_CR33","doi-asserted-by":"crossref","first-page":"e1003701","DOI":"10.1371\/journal.pgen.1003701","volume":"9","author":"KM Glover-Cutter","year":"2013","unstructured":"Glover-Cutter, K. M., Lin, S. & Blackwell, T. K. Integration of the unfolded protein and oxidative stress responses through SKN-1\/Nrf. PLoS Genet. 9, e1003701 (2013).","journal-title":"PLoS Genet."},{"key":"BFsrep21611_CR34","doi-asserted-by":"crossref","first-page":"7540","DOI":"10.1073\/pnas.92.16.7540","volume":"92","author":"GJ Lithgow","year":"1995","unstructured":"Lithgow, G. J., White, T. M., Melov, S. & Johnson, T. E. Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc. Natl. Acad. Sci. USA. 92, 7540\u20137544 (1995).","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"BFsrep21611_CR35","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1093\/genetics\/163.1.171","volume":"163","author":"MJ Mu\u00f1oz","year":"2003","unstructured":"Mu\u00f1oz, M. J. & Riddle, D. L. Positive selection of Caenorhabditis elegans mutants with increased stress resistance and longevity. Genetics 163, 171\u2013180 (2003).","journal-title":"Genetics"},{"key":"BFsrep21611_CR36","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1042\/BJ20070521","volume":"409","author":"NW Kahn","year":"2008","unstructured":"Kahn, N. W., Rea, S. L., Moyle, S., Kell, A. & Johnson, T. E. Proteasomal dysfunction activates the transcription factor SKN-1 and produces a selective oxidative-stress response in Caenorhabditis elegans. Biochem. J. 409, 205\u2013213 (2008).","journal-title":"Biochem. J."},{"key":"BFsrep21611_CR37","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1128\/MCB.01811-08","volume":"29","author":"KP Choe","year":"2009","unstructured":"Choe, K. P., Przybysz, A. J. & Strange, K. The WD40 repeat protein WDR-23 functions with the CUL4\/DDB1 ubiquitin ligase to regulate nuclear abundance and activity of SKN-1 in Caenorhabditis elegans. Mol. Cell Biol. 29, 2704\u20132715 (2009).","journal-title":"Mol. Cell Biol."},{"key":"BFsrep21611_CR38","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature09873","volume":"472","author":"S Alavez","year":"2011","unstructured":"Alavez, S., Vantipalli, M. C., Zucker, D. J., Klang, I. M. & Lithgow, G. J. Amyloid-binding compounds maintain protein homeostasis during ageing and extend lifespan. Nature 472, 226\u2013229 (2011).","journal-title":"Nature"},{"key":"BFsrep21611_CR39","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nature05904","volume":"447","author":"NA Bishop","year":"2007","unstructured":"Bishop, N. A. & Guarente, L. Two neurons mediate diet-restriction-induced longevity in C. elegans. Nature 447, 545\u2013549 (2007).","journal-title":"Nature"},{"key":"BFsrep21611_CR40","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.niox.2011.02.007","volume":"25","author":"A Uruno","year":"2011","unstructured":"Uruno, A. & Motohashi, H. The Keap1-Nrf2 system as an in vivo sensor for electrophiles. Nitric Oxide 25, 153\u2013160 (2011).","journal-title":"Nitric Oxide"},{"key":"BFsrep21611_CR41","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3109\/03602532.2012.684799","volume":"44","author":"KP Choe","year":"2012","unstructured":"Choe, K. P., Leung, C. K. & Miyamoto, M. M. Unique structure and regulation of the nematode detoxification gene regulator, SKN-1:implications to understanding and controlling drug resistance. Drug Metab. Rev. 44, 209\u2013223 (2012).","journal-title":"Drug Metab. Rev."},{"key":"BFsrep21611_CR42","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.mad.2005.03.014","volume":"126","author":"G Walker","year":"2005","unstructured":"Walker, G., Houthoofd, K., Vanfleteren, J. R. & Gems, D. Dietary restriction in C. elegans: From rate-of-living effects to nutrient sensing pathways. Mech. Ageing Dev. 126, 929\u2013937 (2005).","journal-title":"Mech. Ageing Dev."},{"key":"BFsrep21611_CR43","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1096\/fj.00-0242fje","volume":"14","author":"J Jiang","year":"2000","unstructured":"Jiang, J., Jaruga, E., Repnevskaya, M. & Jazwinski, S. An intervention resembling caloric restriction prolongs life span and retards aging in yeast. FASEB J. 14, 2135\u20132137 (2000).","journal-title":"FASEB J."},{"key":"BFsrep21611_CR44","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1093\/jn\/116.4.641","volume":"116","author":"R Weindruch","year":"1986","unstructured":"Weindruch, R., Walford, R., Fligiel, S. & Guthrie, D. The retardation of aging in mice by dietary restriction: longevity, cancer, immunity and lifetime energy intake. J. Nutr. 116, 641\u2013654 (1986).","journal-title":"J. Nutr."},{"key":"BFsrep21611_CR45","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/0022-1910(87)90060-6","volume":"33","author":"L Partridge","year":"1987","unstructured":"Partridge, L., Green, A. & Fowler, K. Effects of egg-production and of exposure to males on female survival in Drosophila melanogaster. J. Insect Physiol. 33, 745\u2013749 (1987).","journal-title":"J. Insect Physiol."},{"key":"BFsrep21611_CR46","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF02664770","volume":"35","author":"L Liu","year":"2000","unstructured":"Liu, L. & Yeh, Y. Y. Inhibition of cholesterol biosynthesis by organosulfur compounds derived from garlic. Lipids 35, 197\u2013203 (2000).","journal-title":"Lipids"},{"key":"BFsrep21611_CR47","doi-asserted-by":"crossref","first-page":"11226","DOI":"10.1021\/jf102423w","volume":"58","author":"R Arg\u00fcello-Garc\u00eda","year":"2010","unstructured":"Arg\u00fcello-Garc\u00eda, R., Medina-Campos, O. N., P\u00e9rez-Hern\u00e1ndez, N., Pedraza-Chaverr\u00ed, J. & Ortega-Pierres, G. Hypochlorous acid scavenging activities of thioallyl compounds from garlic. J. Agric. Food Chem. 58, 11226\u201311233 (2010).","journal-title":"J. Agric. Food Chem."},{"key":"BFsrep21611_CR48","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s11745-001-0734-4","volume":"36","author":"L Liu","year":"2001","unstructured":"Liu, L. & Yeh, Y. Y. Water-soluble organosulfur compounds of garlic inhibit fatty acid and triglyceride syntheses in cultured rat hepatocytes. Lipids 36, 395\u2013400 (2001).","journal-title":"Lipids"},{"key":"BFsrep21611_CR49","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.exger.2011.01.005","volume":"46","author":"AA Powolny","year":"2011","unstructured":"Powolny, A. A., Singh, S. V., Melov, S., Hubbard, A. & Fisher, A. L. The garlic constituent diallyl trisulfide increases the lifespan of C. elegans via skn-1 activation. Exp. Gerontol. 46, 441\u2013452 (2011).","journal-title":"Exp. Gerontol."},{"key":"BFsrep21611_CR50","doi-asserted-by":"crossref","unstructured":"Hatono, S. & Wargovich, M. J. Role of garlic in disease prevention \u2013 preclinical models, in Nutraceuticals: Designer food III Garlic, Soy and Licorice ; Lachance P. A., Ed.; Food and Nutrition Press: Trumbull, CT, 139\u2013151 (1997).","DOI":"10.1002\/9780470385043.ch15"},{"key":"BFsrep21611_CR51","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1055\/s-2006-959461","volume":"60","author":"S Nagae","year":"1994","unstructured":"Nagae, S. et al. Pharmacokinetics of the garlic compound S-allylcysteine. Planta Med. 60, 214\u2013217 (1994).","journal-title":"Planta Med."},{"key":"BFsrep21611_CR52","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","volume":"77","author":"S Brenner","year":"1974","unstructured":"Brenner, S. The genetics of Caenorhabditis elegans. Genetics 77, 71\u201394 (1974).","journal-title":"Genetics"},{"key":"BFsrep21611_CR53","doi-asserted-by":"crossref","unstructured":"Solis, G. M. & Petrascheck, M. Measuring Caenorhabditis elegans life span in 96 well microtiter plates. J. Vis. Exp. 49, pii: 2496. 10.3791\/2496 (2011).","DOI":"10.3791\/2496"},{"key":"BFsrep21611_CR54","doi-asserted-by":"crossref","unstructured":"Smith, E. D. et al. Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans. BMC Dev. Biol. 8, 10.1186\/1471-213X-8-49 (2008).","DOI":"10.1186\/1471-213X-8-49"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep21611","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep21611.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep21611.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T13:55:01Z","timestamp":1672840501000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep21611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,22]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,4,20]]}},"alternative-id":["BFsrep21611"],"URL":"https:\/\/doi.org\/10.1038\/srep21611","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,22]]},"assertion":[{"value":"11 August 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 February 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"21611"}}