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We found that Drosophila Rbf1 associates with genes encoding components of the highly conserved apical\u2013basal and planar cell polarity pathways, suggesting a possible regulatory role. Here, we show that depletion of Rbf1 in <jats:italic>Drosophila<\/jats:italic> tissues is indeed associated with polarity defects in the wing and eye. Key polarity genes <jats:italic>aPKC<\/jats:italic>, <jats:italic>par6<\/jats:italic>, <jats:italic>vang<\/jats:italic>, <jats:italic>pk<\/jats:italic> and <jats:italic>fmi<\/jats:italic> are upregulated and an <jats:italic>aPKC<\/jats:italic> mutation suppresses the Rbf1-induced phenotypes. RB control of cell polarity may be an evolutionarily conserved function, with important implications in cancer metastasis.<\/jats:p>","DOI":"10.1038\/srep22879","type":"journal-article","created":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T10:25:26Z","timestamp":1457951126000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein"],"prefix":"10.1038","volume":"6","author":[{"given":"Sandhya","family":"Payankaulam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelvin","family":"Yeung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"McNeill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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