{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:44:05Z","timestamp":1764783845837},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T00:00:00Z","timestamp":1461628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T00:00:00Z","timestamp":1461628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polystyrene sulfonate (PSS), a strong polyelectrolyte, was used to prepare red photoluminescent PSS-penicillamine (PA) copper (Cu) nanoclusters (NC) aggregates, which displayed high selectivity and sensitivity to the detection of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S). The size of the PSS-PA-Cu NC aggregates could be readily controlled from 5.5\u2009\u03bcm to 173\u2009nm using different concentrations of PSS, which enabled better dispersity and higher sensitivity towards H<jats:sub>2<\/jats:sub>S. PSS-PA-Cu NC aggregates provided rapid H<jats:sub>2<\/jats:sub>S detection by using the strong Cu-S interaction to quench NC photoluminescence as a sensing mechanism. As a result, a detection limit of 650\u2009nM, which is lower than the maximum level permitted in drinking water by the World Health Organization, was achieved for the analysis of H<jats:sub>2<\/jats:sub>S in spring-water samples. Moreover, highly dispersed PSS-PA-Cu NC aggregates could be incorporated into a plate-format paper-based analytical device which enables ultra-low sample volumes (5\u2009\u03bcL) and feature shorter analysis times (30\u2009min) compared to conventional solution-based methods. The advantages of low reagent consumption, rapid result readout, limited equipment and long-term storage make this platform sensitive and simple enough to use without specialized training in resource constrained settings.<\/jats:p>","DOI":"10.1038\/srep24882","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T10:12:04Z","timestamp":1461665524000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Size-tunable copper nanocluster aggregates and their application in hydrogen sulfide sensing on paper-based devices"],"prefix":"10.1038","volume":"6","author":[{"given":"Po-Cheng","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Chi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Yin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Yu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien-Fu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan-Tsung","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,4,26]]},"reference":[{"key":"BFsrep24882_CR1","doi-asserted-by":"publisher","first-page":"4691","DOI":"10.1039\/c3nr00713h","volume":"5","author":"P-C Chen","year":"2013","unstructured":"Chen, P.-C., Yeh, T.-Y., Ou, C.-M., Shih, C.-C. & Chang, H.-T. Synthesis of aluminum oxide supported fluorescent gold nanodots for the detection of silver ions. Nanoscale 5, 4691\u20134695 (2013).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR2","doi-asserted-by":"publisher","first-page":"5965","DOI":"10.1021\/acsami.5b00269","volume":"7","author":"L Qin","year":"2015","unstructured":"Qin, L., He, X., Chen, L. & Zhang, Y. Turn-on fluorescent sensing of glutathione s-transferase at near-infrared region based on FRET between gold nanoclusters and gold nanorods. ACS Appl. Mater. Interfaces 7, 5965\u20135971 (2015).","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"BFsrep24882_CR3","doi-asserted-by":"publisher","first-page":"4918","DOI":"10.1021\/nl502720s","volume":"14","author":"N Enkin","year":"2014","unstructured":"Enkin, N., Sharon, E., Golub, E. & Willner, I. Ag nanocluster\/DNA hybrids: functional modules for the detection of nitroaromatic and RDX explosives. Nano Lett. 14, 4918\u20134922 (2014).","journal-title":"Nano Lett."},{"key":"BFsrep24882_CR4","doi-asserted-by":"publisher","first-page":"15608","DOI":"10.1038\/srep15608","volume":"5","author":"A Senthamizhan","year":"2015","unstructured":"Senthamizhan, A., Celebioglu, A., Balusamy, B. & Uyar, T. Immobilization of gold nanoclusters inside porous electrospun fibers for selective detection of Cu (II): a strategic approach to shielding pristine performance. Sci. Rep. 5, 15608 (2015).","journal-title":"Sci. Rep"},{"key":"BFsrep24882_CR5","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1021\/cs300252g","volume":"2","author":"S Xie","year":"2012","unstructured":"Xie, S., Tsunoyama, H., Kurashige, W., Negishi, Y. & Tsukuda, T. Enhancement in aerobic alcohol oxidation catalysis of Au25 clusters by single Pd atom doping. ACS Catal. 2, 1519\u20131523 (2012).","journal-title":"ACS Catal"},{"key":"BFsrep24882_CR6","doi-asserted-by":"publisher","first-page":"6014","DOI":"10.1021\/nn301019f","volume":"6","author":"X Nie","year":"2012","unstructured":"Nie, X., Qian, H., Ge, Q., Xu, H. & Jin, R. CO oxidation catalyzed by oxide-supported Au25(SR)18 nanoclusters and identification of perimeter sites as active centers. ACS Nano 6, 6014\u20136022 (2012).","journal-title":"ACS Nano"},{"key":"BFsrep24882_CR7","doi-asserted-by":"publisher","first-page":"4384","DOI":"10.1038\/srep04384","volume":"4","author":"S Gao","year":"2014","unstructured":"Gao, S. et al. Near-infrared fluorescence imaging of cancer cells and tumors through specific biosynthesis of silver nanoclusters. Sci. Rep. 4, 4384 (2014).","journal-title":"Sci. Rep"},{"key":"BFsrep24882_CR8","doi-asserted-by":"publisher","first-page":"8571","DOI":"10.1039\/C4CC03949A","volume":"50","author":"Y-N Chen","year":"2014","unstructured":"Chen, Y.-N. et al. One-pot synthesis of fluorescent BSA-Ce\/Au nanoclusters as ratiometric pH probes. Chem. Commun. 50, 8571\u20138574 (2014).","journal-title":"Chem. Commun."},{"key":"BFsrep24882_CR9","doi-asserted-by":"publisher","first-page":"9718","DOI":"10.1021\/nn2032177","volume":"5","author":"YL Wang","year":"2011","unstructured":"Wang, Y. L., Chen, J. J. & Irudayaraj, J. Nuclear targeting dynamics of gold nanoclusters for enhanced therapy of HER2+ breast cancer. ACS Nano 5, 9718\u20139725 (2011).","journal-title":"ACS Nano"},{"key":"BFsrep24882_CR10","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1039\/B9NR00160C","volume":"2","author":"R Jin","year":"2010","unstructured":"Jin, R. Quantum sized, thiolate-protected gold nanoclusters. Nanoscale 2, 343\u2013362 (2010).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR11","doi-asserted-by":"publisher","first-page":"4073","DOI":"10.1039\/c2nr31192e","volume":"4","author":"J Zheng","year":"2012","unstructured":"Zheng, J., Zhou, C., Yu, M. & Liu, J. Different sized luminescent gold nanoparticles. Nanoscale 4, 4073\u20134083 (2012).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR12","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1021\/ja806804u","volume":"131","author":"J Xie","year":"2009","unstructured":"Xie, J., Zheng, Y. & Ying, J. Y. Protein-directed synthesis of highly fluorescent gold nanoclusters. J. Am. Chem. Soc. 131, 888\u2013889 (2009).","journal-title":"J. Am. Chem. Soc."},{"key":"BFsrep24882_CR13","doi-asserted-by":"publisher","first-page":"6824","DOI":"10.1002\/anie.200700803","volume":"46","author":"CC Huang","year":"2007","unstructured":"Huang, C. C., Yang, Z., Lee, K. H. & Chang, H. T. Synthesis of highly fluorescent gold nanoparticles for sensing Mercury(II). Angew. Chem., Int. Ed. 46, 6824\u20136828 (2007).","journal-title":"Angew. Chem., Int. Ed."},{"key":"BFsrep24882_CR14","doi-asserted-by":"publisher","first-page":"16662","DOI":"10.1021\/ja306199p","volume":"134","author":"Z Luo","year":"2012","unstructured":"Luo, Z. et al. From aggregation-induced emission of Au(I)\u2013thiolate complexes to ultrabright Au(0)@Au(I)\u2013thiolate core\u2013shell nanoclusters. J. Am. Chem. Soc. 134, 16662\u201316670 (2012).","journal-title":"J. Am. Chem. Soc."},{"key":"BFsrep24882_CR15","doi-asserted-by":"publisher","first-page":"3873","DOI":"10.1002\/smll.201300896","volume":"9","author":"X Jia","year":"2013","unstructured":"Jia, X., Li, J. & Wang, E. Cu nanoclusters with aggregation induced emission enhancement. Small 9, 3873\u20133879 (2013).","journal-title":"Small"},{"key":"BFsrep24882_CR16","doi-asserted-by":"publisher","first-page":"4190","DOI":"10.1039\/c2nr30253e","volume":"4","author":"EB Guidez","year":"2012","unstructured":"Guidez, E. B. & Aikens, C. M. Theoretical analysis of the optical excitation spectra of silver and gold nanowires. Nanoscale 4, 4190\u20134198 (2012).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR17","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1161\/CIRCRESAHA.114.300505","volume":"114","author":"DJ Polhemus","year":"2014","unstructured":"Polhemus, D. J. & Lefer, D. J. Emergence of hydrogen sulfide as an endogenous gaseous signaling molecule in cardiovascular disease. Circ. Res. 114, 730\u2013737 (2014).","journal-title":"Circ. Res."},{"key":"BFsrep24882_CR18","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1039\/b003192p","volume":"11","author":"W Cheng","year":"2001","unstructured":"Cheng, W., Baudrin, E., Dunn, B. & Zink, J. I. Synthesis and electrochromic properties of mesoporous tungsten oxideBasis of a presentation given at Materials Discussion No. 3, 26\u201329 September, 2000, University of Cambridge, UK. J. Mater. Chem. 11, 92\u201397 (2001).","journal-title":"J. Mater. Chem"},{"key":"BFsrep24882_CR19","doi-asserted-by":"publisher","first-page":"8719","DOI":"10.1038\/srep08719","volume":"5","author":"H Shafiee","year":"2015","unstructured":"Shafiee, H. et al. Paper and flexible substrates as materials for biosensing platforms to detect multiple biotargets. Sci. Rep. 5, 8719 (2015).","journal-title":"Sci. Rep"},{"key":"BFsrep24882_CR20","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1021\/ac503968p","volume":"87","author":"DM Cate","year":"2014","unstructured":"Cate, D. M., Adkins, J. A., Mettakoonpitak, J. & Henry, C. S. Recent developments in paper-based microfluidic devices. Anal. Chem. 87, 19\u201341 (2014).","journal-title":"Anal. Chem."},{"key":"BFsrep24882_CR21","doi-asserted-by":"publisher","first-page":"7091","DOI":"10.1021\/ac901071p","volume":"81","author":"E Carrilho","year":"2009","unstructured":"Carrilho, E., Martinez, A. W. & Whitesides, G. M. Understanding wax printing: a simple micropatterning process for paper-based microfluidics. Anal. Chem. 81, 7091\u20137095 (2009).","journal-title":"Anal. Chem."},{"key":"BFsrep24882_CR22","doi-asserted-by":"publisher","first-page":"15023","DOI":"10.1021\/acsami.5b04104","volume":"7","author":"P Liang","year":"2015","unstructured":"Liang, P., Yu, H., Guntupalli, B. & Xiao, Y. Paper-based device for rapid visualization of NADH based on sissolution of gold nanoparticles. ACS Appl. Mater. Interfaces 7, 15023\u201315030 (2015).","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"BFsrep24882_CR23","doi-asserted-by":"publisher","first-page":"6843","DOI":"10.1021\/ac5008688","volume":"86","author":"G-H Chen","year":"2014","unstructured":"Chen, G.-H. et al. Detection of mercury (II) ions using colorimetric gold nanoparticles on paper-based analytical devices. Anal. Chem. 86, 6843\u20136849 (2014).","journal-title":"Anal. Chem."},{"key":"BFsrep24882_CR24","doi-asserted-by":"publisher","first-page":"195502","DOI":"10.1088\/0957-4484\/25\/19\/195502","volume":"25","author":"J-Y Ma","year":"2014","unstructured":"Ma, J.-Y., Chen, P.-C. & Chang, H.-T. Detection of hydrogen sulfide through photoluminescence quenching of penicillamine-copper nanocluster aggregates. Nanotechnology 25, 195502 (2014).","journal-title":"Nanotechnology"},{"key":"BFsrep24882_CR25","doi-asserted-by":"publisher","first-page":"6162","DOI":"10.1021\/nn402037x","volume":"7","author":"H Ahn","year":"2013","unstructured":"Ahn, H. et al. Blue-emitting self-assembled polymer electrolytes for fast, sensitive, label-free detection of Cu(II) ions in aqueous media. ACS Nano 7, 6162\u20136169 (2013).","journal-title":"ACS Nano"},{"key":"BFsrep24882_CR26","doi-asserted-by":"publisher","first-page":"8847","DOI":"10.1364\/OE.19.008847","volume":"19","author":"C-C Chiang","year":"2011","unstructured":"Chiang, C.-C. et al. Optical tweezers based active microrheology of sodium polystyrene sulfonate (NaPSS). Opt. Express 19, 8847\u20138854 (2011).","journal-title":"Opt. Express"},{"key":"BFsrep24882_CR27","doi-asserted-by":"publisher","first-page":"5261","DOI":"10.1021\/ja042218h","volume":"127","author":"Y Negishi","year":"2005","unstructured":"Negishi, Y., Nobusada, K. & Tsukuda, T. Glutathione-protected gold clusters revisited: Bridging the gap between gold(I)-thiolate complexes and thiolate-protected gold nanocrystals. J. Am. Chem. Soc. 127, 5261\u20135270 (2005).","journal-title":"J. Am. Chem. Soc."},{"key":"BFsrep24882_CR28","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1039\/C3CC47771A","volume":"50","author":"X Jia","year":"2014","unstructured":"Jia, X., Yang, X., Li, J., Li, D. & Wang, E. Stable Cu nanoclusters: from an aggregation-induced emission mechanism to biosensing and catalytic applications. Chem. Comm. 50, 237\u2013239 (2014).","journal-title":"Chem. Comm"},{"key":"BFsrep24882_CR29","doi-asserted-by":"publisher","first-page":"2060","DOI":"10.1021\/ja109303z","volume":"133","author":"W Wei","year":"2011","unstructured":"Wei, W., Lu, Y., Chen, W. & Chen, S. One-pot synthesis, photoluminescence and electrocatalytic properties of subnanometer-sized copper clusters. J. Am. Chem. Soc. 133, 2060\u20132063 (2011).","journal-title":"J. Am. Chem. Soc."},{"key":"BFsrep24882_CR30","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1039\/c0nr00947d","volume":"3","author":"L Shang","year":"2011","unstructured":"Shang, L. et al. Facile preparation of water-soluble fluorescent gold nanoclusters for cellular imaging applications. Nanoscale 3, 2009\u20132014 (2011).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR31","doi-asserted-by":"publisher","first-page":"035701","DOI":"10.1088\/0957-4484\/19\/03\/035701","volume":"19","author":"DL Nida","year":"2008","unstructured":"Nida, D. L., Nitin, N., Yu, W. W., Colvin, V. L. & Richards-Kortum, R. Photostability of quantum dots with amphiphilic polymer-based passivation strategies. Nanotechnology 19, 035701 (2008).","journal-title":"Nanotechnology"},{"key":"BFsrep24882_CR32","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S0021-9673(98)00882-6","volume":"830","author":"PR B\u00e9rub\u00e9","year":"1999","unstructured":"B\u00e9rub\u00e9, P. R., Parkinson, P. D. & Hall, E. R. Measurement of reduced sulphur compounds contained in aqueous matrices by direct injection into a gas chromatograph with a flame photometric detector. J. Chromatogr. A 830, 485\u2013489 (1999).","journal-title":"J. Chromatogr. A"},{"key":"BFsrep24882_CR33","doi-asserted-by":"crossref","unstructured":"Choi, M. G., Cha, S., Lee, H., Jeon, H. L. & Chang, S.-K. Sulfide-selective chemosignaling by a Cu2+ complex of dipicolylamine appended fluorescein. Chem. Commun., 7390\u20137392 (2009).","DOI":"10.1039\/b916476f"},{"key":"BFsrep24882_CR34","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1039\/a801702f","volume":"123","author":"M Choi","year":"1998","unstructured":"Choi, M. Fluorimetric optode membrane for sulfide detection. Analyst 123, 1631\u20131634 (1998).","journal-title":"Analyst"},{"key":"BFsrep24882_CR35","doi-asserted-by":"publisher","first-page":"10834","DOI":"10.1021\/ac502941h","volume":"86","author":"E Bitziou","year":"2014","unstructured":"Bitziou, E. et al. In situ optimization of pH for parts-per-billion electrochemical detection of dissolved hydrogen sulfide using boron doped diamond flow electrodes. Anal. Chem. 86, 10834\u201310840 (2014).","journal-title":"Anal. Chem."},{"key":"BFsrep24882_CR36","doi-asserted-by":"publisher","first-page":"2604","DOI":"10.1021\/nl1013184","volume":"10","author":"L Mai","year":"2010","unstructured":"Mai, L. et al. Single \u03b2-AgVO3 nanowire H2S sensor. Nano Lett. 10, 2604\u20132608 (2010).","journal-title":"Nano Lett."},{"key":"BFsrep24882_CR37","doi-asserted-by":"publisher","first-page":"14975","DOI":"10.1021\/am502671s","volume":"6","author":"S Zhang","year":"2014","unstructured":"Zhang, S. et al. Facile fabrication of a well-ordered porous Cu-doped SnO2 Thin Film for H2S Sensing. ACS Appl. Mater. Interfaces 6, 14975\u201314980 (2014).","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"BFsrep24882_CR38","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.trac.2011.08.008","volume":"32","author":"SK Pandey","year":"2012","unstructured":"Pandey, S. K., Kim, K.-H. & Tang, K.-T. A review of sensor-based methods for monitoring hydrogen sulfide. Trends Anal. Chem. 32, 87\u201399 (2012).","journal-title":"Trends Anal. Chem."},{"key":"BFsrep24882_CR39","doi-asserted-by":"publisher","first-page":"1732","DOI":"10.1002\/ange.201207701","volume":"125","author":"Y Chen","year":"2013","unstructured":"Chen, Y. et al. A ratiometric fluorescent probe for rapid detection of hydrogen sulfide in mitochondria. Angew. Chem., Int. Ed. 125, 1732\u20131735 (2013).","journal-title":"Angew. Chem., Int. Ed."},{"key":"BFsrep24882_CR40","doi-asserted-by":"publisher","first-page":"2818","DOI":"10.1021\/ja411067a","volume":"136","author":"J Liu","year":"2014","unstructured":"Liu, J. et al. Selective Ag (I) binding, H2S sensing and white-light emission from an easy-to-make porous conjugated polymer. J. Am. Chem. Soc. 136, 2818\u20132824 (2014).","journal-title":"J. Am. Chem. Soc."},{"key":"BFsrep24882_CR41","doi-asserted-by":"publisher","first-page":"4683","DOI":"10.1039\/c2nr33202g","volume":"5","author":"Z Yuan","year":"2013","unstructured":"Yuan, Z. et al. Disassembly mediated fluorescence recovery of gold nanodots for selective sulfide sensing. Nanoscale 5, 4683\u20134686 (2013).","journal-title":"Nanoscale"},{"key":"BFsrep24882_CR42","doi-asserted-by":"publisher","first-page":"9","DOI":"10.3891\/acta.chem.scand.11-0731","volume":"11","author":"G Biederman","year":"1957","unstructured":"Biederman, G. & Schindler, P. On the solubility product of precipitated iron hydroxide. Acta Chem. Scand. 11, 9\u201315 (1957).","journal-title":"Acta Chem. Scand."},{"key":"BFsrep24882_CR43","doi-asserted-by":"publisher","first-page":"9139","DOI":"10.1021\/acsami.5b01192","volume":"7","author":"RH Lahr","year":"2015","unstructured":"Lahr, R. H., Wallace, G. C. & Vikesland, P. J. Raman characterization of nanoparticle transport in microfluidic paper-based analytical devices (\u03bcPADs). ACS Appl. Mater. Interfaces 7, 9139\u20139146 (2015).","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"BFsrep24882_CR44","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1039\/C4LC01239A","volume":"15","author":"AK Badu-Tawiah","year":"2015","unstructured":"Badu-Tawiah, A. K. et al. Polymerization-based signal amplification for paper-based immunoassays. Lab Chip 15, 655\u2013659 (2015).","journal-title":"Lab Chip"},{"key":"BFsrep24882_CR45","unstructured":"Eaton, A., Clesceri, L. S., Rice, E. W., Greenberg, A. E. & Franson, M. APHA: Standard methods for the xxamination of water and wastewater. Centennial Edition., APHA, AWWA, WEF, Washington, DC (2005)."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep24882","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep24882.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep24882.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T14:12:40Z","timestamp":1672841560000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep24882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,26]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,7,6]]}},"alternative-id":["BFsrep24882"],"URL":"https:\/\/doi.org\/10.1038\/srep24882","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,26]]},"assertion":[{"value":"11 January 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 April 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"24882"}}