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Oxidative stress has been proposed to be involved in eye diseases, including cataract formation. We visualized the distribution of metals and other trace elements in the eye of zebrafish embryos by micro X-ray fluorescence (\u03bc-XRF) imaging. Many elements showed highest accumulation in the retinal pigment epithelium (RPE) of the zebrafish embryo. Knockdown of the zebrafish <jats:italic>brown<\/jats:italic> locus homologues <jats:italic>tyrp1a\/b<\/jats:italic> eliminated accumulation of these elements in the RPE, indicating that they are bound by mature melanosomes. Furthermore, <jats:italic>albino<\/jats:italic> (<jats:italic>slc45a2<\/jats:italic>) mutants, which completely lack melanosomes, developed abnormal lens reflections similar to the congenital cataract caused by mutation of the myosin chaperon Unc45b, and an <jats:italic>in situ<\/jats:italic> spin trapping assay revealed increased oxidative stress in the lens of <jats:italic>albino<\/jats:italic> mutants. Finally transplanting a wildtype lens into an <jats:italic>albino<\/jats:italic> mutant background resulted in cataract formation. 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