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In this study, 25 IncHI2 plasmids harboring <jats:italic>oqxAB<\/jats:italic> (20\/25)<jats:italic>\/bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> (18\/25) were found with sizes ranging from \u223c260 to \u223c350\u2009kb and 22 belonged to the ST3-IncHI2 group. In addition to <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> and <jats:italic>oqxAB<\/jats:italic>, <jats:italic>pcoA<\/jats:italic>-<jats:italic>E<\/jats:italic> (5\/25) and <jats:italic>silE<\/jats:italic>-<jats:italic>P<\/jats:italic> (5\/25), as well as <jats:italic>aac<\/jats:italic>(<jats:italic>6<\/jats:italic>\u2032)<jats:italic>-Ib-cr<\/jats:italic> (18\/25), <jats:italic>floR<\/jats:italic> (16\/25), <jats:italic>rmtB<\/jats:italic> (6\/25), <jats:italic>qnrS1<\/jats:italic>(3\/25) and <jats:italic>fosA3<\/jats:italic> (2\/25), were also identified on these IncHI2 plasmids. The plasmids carried <jats:italic>pco<\/jats:italic> and <jats:italic>sil<\/jats:italic> contributed to increasing in the MICs of CuSO<jats:sub>4<\/jats:sub> and AgNO<jats:sub>3<\/jats:sub>. The genetic context surrounding the two operons was well conserved except some variations within the <jats:italic>pco<\/jats:italic> operon. The ~32\u2009kb region containing the two operons identified in the IncHI2 plasmids was also found in chromosomes of different Enterobacteriaceae species. Further, phylogenetic analysis of this structure showed that Tn7-like transposon might play an important role in cross-genus transfer of the <jats:italic>sil<\/jats:italic> and <jats:italic>pco<\/jats:italic> operons among Enterobacteriaceae. In conclusion, co-existence of the <jats:italic>pco<\/jats:italic> and <jats:italic>sil<\/jats:italic> operons, and <jats:italic>oqxAB\/bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> as well as other antibiotic resistance genes on IncHI2 plasmids may promote the development of multidrug-resistant bacteria.<\/jats:p>","DOI":"10.1038\/srep25312","type":"journal-article","created":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T09:23:23Z","timestamp":1462353803000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":148,"title":["Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals"],"prefix":"10.1038","volume":"6","author":[{"given":"Liangxing","family":"Fang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,5,4]]},"reference":[{"key":"BFsrep25312_CR1","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1093\/jac\/dks114","volume":"67","author":"L Villa","year":"2012","unstructured":"Villa, L., Poirel, L., Nordmann, P., Carta, C. & Carattoli, A. 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