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ASGP-Rs are well known to specifically express in the mammalian liver. Here, we demonstrated ASGP-R expression decreased in carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)-induced mouse model. ASGP-R expression correlated with liver fibrosis progression. ASGP-R could be a useful marker in the stage of liver fibrosis. Liver uptake value (LUV) derived by SPECT imaging was used to assess liver fibrosis in the CCl<jats:sub>4<\/jats:sub>-induced mouse model. LUV\u2009=\u2009[radioactivity (liver uptake)\/radioactivity (injected)]\u2009\u00d7\u2009100\/liver volume. The LUV decreased along with the disease progression. The relationships between LUV and liver hydroxyproline (i.e. collagen), as well as Sirius Red were established and verified. A strong negative linear correlation was found between LUV and hydroxyproline levels (r\u2009=\u2009\u22120.83) as well as LUV and Sirius Red quantification (r\u2009=\u2009\u22120.83). 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