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Using a <jats:italic>Borrelia burgdorferi<\/jats:italic>-based heterologous system and gain-in-function approach, we show for the first time that a highly immunogenic lipoprotein TP0435 can be differentially processed into multiple isoforms with one variant stochastically displayed on the spirochete surface. TP0435 was previously believed to be exclusively located in <jats:italic>T. pallidum<\/jats:italic> periplasm. Furthermore, non-adherent <jats:italic>B. burgdorferi<\/jats:italic> strain expressing TP0435 acquires the ability to bind to a variety of host cells including placental cells and exhibits slow opsonophagocytosis <jats:italic>in vitro<\/jats:italic> similar to poor <jats:italic>ex vivo<\/jats:italic> phagocytosis of <jats:italic>T. pallidum<\/jats:italic> by host macrophages reported previously. This phenomenon of production of both surface and periplasmic immunogenic lipoprotein isoforms has possible implications in immune evasion of the obligate pathogen <jats:italic>T. pallidum<\/jats:italic> during infection.<\/jats:p>","DOI":"10.1038\/srep25593","type":"journal-article","created":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T09:43:13Z","timestamp":1462873393000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Treponema pallidum Lipoprotein TP0435 Expressed in Borrelia burgdorferi Produces Multiple Surface\/Periplasmic Isoforms and mediates Adherence"],"prefix":"10.1038","volume":"6","author":[{"given":"Kamfai","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thayer","family":"Nasereddin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Alter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Centurion-Lara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Giacani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhat","family":"Parveen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,5,10]]},"reference":[{"key":"BFsrep25593_CR1","first-page":"S12","volume":"74","author":"AC Gerbase","year":"1998","unstructured":"Gerbase, A. 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