{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:03:39Z","timestamp":1790597019509,"version":"4.1.0"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T00:00:00Z","timestamp":1463011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T00:00:00Z","timestamp":1463011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gout is characterized by the monosodium urate monohydrate (MSU)-induced arthritis. Alpha kinase-1 (ALPK1) has shown to be associated with MSU-induced inflammation and gout. Here, we used bioinformatics, proteomics, cell models, and twenty <jats:italic>in vitro<\/jats:italic> human assays to clarify some of its role in the inflammatory response to MSU. We found myosin IIA to be a frequent interacting protein partner of ALPK1, binding to its N-terminal and forming a protein complex with calmodulin and F-actin, and that MSU-induced ALPK1 phosphorylated the myosin IIA. A knockdown of endogenous ALPK1 or myosin IIA significantly reduced the MSU-induced secretion of tumour necrosis factor (TNF)-\u03b1. Furthermore, all gouty patients expressed higher basal protein levels of ALPK1, myosin IIA, and plasma TNF-\u03b1, however those medicated with colchicine has shown reduced myosin IIA and TNF-\u03b1 but not ALPK1. The findings suggest ALPK1 is a kinase that participates in the regulation of Golgi-derived TNF-\u03b1 trafficking through myosin IIA phosphorylation in the inflammation of gout. This novel pathway could be blocked at the level of myosin by colchicine in gout treatment.<\/jats:p>","DOI":"10.1038\/srep25740","type":"journal-article","created":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T10:12:21Z","timestamp":1463047941000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["ALPK1 phosphorylates myosin IIA modulating TNF-\u03b1 trafficking in gout flares"],"prefix":"10.1038","volume":"6","author":[{"given":"Chi-Pin","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang-Lun","family":"Chiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert Min-Shan","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Fan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Che","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Yu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Ming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Gowth","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzer-Min","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Lin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eing-Mei","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chin","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,5,12]]},"reference":[{"key":"BFsrep25740_CR1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1086\/423429","volume":"75","author":"LS Cheng","year":"2004","unstructured":"Cheng, L. S. et al. Genomewide scan for gout in taiwanese aborigines reveals linkage to chromosome 4q25. Am J Hum Genet 75, 498\u2013503 (2004).","journal-title":"Am J Hum Genet"},{"key":"BFsrep25740_CR2","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1093\/ije\/dyt028","volume":"42","author":"AM Ko","year":"2013","unstructured":"Ko, A. M. et al. ALPK1 genetic regulation and risk in relation to gout. Int J Epidemiol 42, 466\u2013474 (2013).","journal-title":"Int J Epidemiol"},{"key":"BFsrep25740_CR3","doi-asserted-by":"publisher","first-page":"1241","DOI":"10.1007\/s00109-011-0796-5","volume":"89","author":"SJ Wang","year":"2011","unstructured":"Wang, S. J. et al. Lymphocyte alpha-kinase is a gout-susceptible gene involved in monosodium urate monohydrate-induced inflammatory responses. J Mol Med (Berl) 89, 1241\u20131251 (2011).","journal-title":"J Mol Med (Berl)"},{"key":"BFsrep25740_CR4","doi-asserted-by":"crossref","first-page":"10082","DOI":"10.1016\/S0021-9258(19)81480-1","volume":"263","author":"JP Vaillancourt","year":"1988","unstructured":"Vaillancourt, J. P., Lyons, C. & Cote, G. P. Identification of two phosphorylated threonines in the tail region of Dictyostelium myosin II. J Biol Chem 263, 10082\u201310087 (1988).","journal-title":"J Biol Chem"},{"key":"BFsrep25740_CR5","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1074\/jbc.270.2.523","volume":"270","author":"LM Futey","year":"1995","unstructured":"Futey, L. M., Medley, Q. G., Cote, G. P. & Egelhoff, T. T. Structural analysis of myosin heavy chain kinase A from Dictyostelium. Evidence for a highly divergent protein kinase domain, an amino-terminal coiled-coil domain, and a domain homologous to the beta-subunit of heterotrimeric G proteins. J Biol Chem. 270, 523\u2013529 (1995).","journal-title":"J Biol Chem."},{"key":"BFsrep25740_CR6","doi-asserted-by":"publisher","first-page":"25637","DOI":"10.1074\/jbc.M502265200","volume":"280","author":"M Heine","year":"2005","unstructured":"Heine, M. et al. Alpha-kinase 1, a new component in apical protein transport. J Biol Chem. 280, 25637\u201325643 (2005).","journal-title":"J Biol Chem."},{"key":"BFsrep25740_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2202-12-1","volume":"12","author":"M Chen","year":"2011","unstructured":"Chen, M. & Xu, R. Motor coordination deficits in Alpk1 mutant mice with the inserted piggyBac transposon. BMC Neurosci 12, 1 (2011).","journal-title":"BMC Neurosci"},{"key":"BFsrep25740_CR8","doi-asserted-by":"publisher","first-page":"499","DOI":"10.7326\/0003-4819-143-7-200510040-00009","volume":"143","author":"HK Choi","year":"2005","unstructured":"Choi, H. K., Mount, D. B. & Reginato, A. M. American College of, P. & American Physiological, S. Pathogenesis of gout. Annals of internal medicine 143, 499\u2013516 (2005).","journal-title":"Annals of internal medicine"},{"key":"BFsrep25740_CR9","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1038\/nature04516","volume":"440","author":"F Martinon","year":"2006","unstructured":"Martinon, F., Petrilli, V., Mayor, A., Tardivel, A. & Tschopp, J. Gout-associated uric acid crystals activate the NALP3 inflammasome. Nature 440, 237\u2013241 (2006).","journal-title":"Nature"},{"key":"BFsrep25740_CR10","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1002\/art.1780400525","volume":"40","author":"PT Chapman","year":"1997","unstructured":"Chapman, P. T. et al. Endothelial activation in monosodium urate monohydrate crystal-induced inflammation: in vitro and in vivo studies on the roles of tumor necrosis factor alpha and interleukin-1. Arthritis Rheum. 40, 955\u2013965 (1997).","journal-title":"Arthritis Rheum."},{"key":"BFsrep25740_CR11","first-page":"218","volume":"25","author":"AC Stanley","year":"2010","unstructured":"Stanley, A. C. & Lacy, P. Pathways for cytokine secretion. Physiology (Bethesda) 25, 218\u2013229 (2010).","journal-title":"Physiology (Bethesda)"},{"key":"BFsrep25740_CR12","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/S1097-2765(01)00256-8","volume":"7","author":"H Yamaguchi","year":"2001","unstructured":"Yamaguchi, H., Matsushita, M., Nairn, A. C. & Kuriyan, J. Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity. Mol Cell 7, 1047\u20131057 (2001).","journal-title":"Mol Cell"},{"key":"BFsrep25740_CR13","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1038\/ncb1763","volume":"10","author":"CK Choi","year":"2008","unstructured":"Choi, C. K. et al. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat Cell Biol. 10, 1039\u20131050 (2008).","journal-title":"Nat Cell Biol."},{"key":"BFsrep25740_CR14","doi-asserted-by":"publisher","first-page":"2285","DOI":"10.1084\/jem.20071143","volume":"204","author":"MM Andzelm","year":"2007","unstructured":"Andzelm, M. M., Chen, X., Krzewski, K., Orange, J. S. & Strominger, J. L. Myosin IIA is required for cytolytic granule exocytosis in human NK cells. J Exp Med 204, 2285\u20132291 (2007).","journal-title":"J Exp Med"},{"key":"BFsrep25740_CR15","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1523\/JNEUROSCI.2818-08.2009","volume":"29","author":"K Berberian","year":"2009","unstructured":"Berberian, K., Torres, A. J., Fang, Q., Kisler, K. & Lindau, M. F-actin and myosin II accelerate catecholamine release from chromaffin granules. J Neurosci 29, 863\u2013870 (2009).","journal-title":"J Neurosci"},{"key":"BFsrep25740_CR16","doi-asserted-by":"publisher","first-page":"e100757","DOI":"10.1371\/journal.pone.0100757","volume":"9","author":"JA Flores","year":"2014","unstructured":"Flores, J. A. et al. A new role for myosin II in vesicle fission. PLoS One 9, e100757 (2014).","journal-title":"PLoS One"},{"key":"BFsrep25740_CR17","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1038\/ncb0710-635","volume":"12","author":"C Valente","year":"2010","unstructured":"Valente, C., Polishchuk, R. & De Matteis, M. A. Rab6 and myosin II at the cutting edge of membrane fission. Nat Cell Biol 12, 635\u2013638 (2010).","journal-title":"Nat Cell Biol"},{"key":"BFsrep25740_CR18","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1091\/mbc.e03-06-0430","volume":"15","author":"T Togo","year":"2004","unstructured":"Togo, T. & Steinhardt, R. A. Nonmuscle myosin IIA and IIB have distinct functions in the exocytosis-dependent process of cell membrane repair. Mol Biol Cell 15, 688\u2013695 (2004).","journal-title":"Mol Biol Cell"},{"key":"BFsrep25740_CR19","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1091\/mbc.e08-10-1048","volume":"20","author":"P Bhat","year":"2009","unstructured":"Bhat, P. & Thorn, P. Myosin 2 maintains an open exocytic fusion pore in secretory epithelial cells. Mol Biol Cell 20, 1795\u20131803 (2009).","journal-title":"Mol Biol Cell"},{"key":"BFsrep25740_CR20","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1042\/BST0391115","volume":"39","author":"LM Bond","year":"2011","unstructured":"Bond, L. M., Brandstaetter, H., Sellers, J. R., Kendrick-Jones, J. & Buss, F. Myosin motor proteins are involved in the final stages of the secretory pathways. Biochem Soc Trans 39, 1115\u20131119 (2011).","journal-title":"Biochem Soc Trans"},{"key":"BFsrep25740_CR21","doi-asserted-by":"publisher","first-page":"27450","DOI":"10.1074\/jbc.M311462200","volume":"279","author":"P Neco","year":"2004","unstructured":"Neco, P. et al. New roles of myosin II during vesicle transport and fusion in chromaffin cells. J Biol Chem 279, 27450\u201327457 (2004).","journal-title":"J Biol Chem"},{"key":"BFsrep25740_CR22","doi-asserted-by":"publisher","first-page":"2956","DOI":"10.1091\/mbc.e14-04-0894","volume":"25","author":"H Yashiro","year":"2014","unstructured":"Yashiro, H., Loza, A. J., Skeath, J. B. & Longmore, G. D. Rho1 Regulates Adherens Junction Remodeling by Promoting Recycling Endosome Formation through Activation of Myosin II. Mol Biol Cell 25, 2956\u20132969 (2014).","journal-title":"Mol Biol Cell"},{"key":"BFsrep25740_CR23","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1182\/blood-2013-06-508168","volume":"123","author":"F Pertuy","year":"2014","unstructured":"Pertuy, F. et al. Myosin IIA is critical for organelle distribution and F-actin organization in megakaryocytes and platelets. Blood 123, 1261\u20131269 (2014).","journal-title":"Blood"},{"key":"BFsrep25740_CR24","doi-asserted-by":"publisher","first-page":"e40814","DOI":"10.1371\/journal.pone.0040814","volume":"7","author":"M Shutova","year":"2012","unstructured":"Shutova, M., Yang, C., Vasiliev, J. M. & Svitkina, T. Functions of nonmuscle myosin II in assembly of the cellular contractile system. PLoS One 7, e40814 (2012).","journal-title":"PLoS One"},{"key":"BFsrep25740_CR25","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1038\/emm.2006.22","volume":"38","author":"YO Choi","year":"2006","unstructured":"Choi, Y. O. et al. Implication of phosphorylation of the myosin II regulatory light chain in insulin-stimulated GLUT4 translocation in 3T3-F442A adipocytes. Exp Mol Med 38, 180\u2013189 (2006).","journal-title":"Exp Mol Med"},{"key":"BFsrep25740_CR26","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1189\/jlb.0711335","volume":"91","author":"JL Maravillas-Montero","year":"2012","unstructured":"Maravillas-Montero, J. L. & Santos-Argumedo, L. The myosin family: unconventional roles of actin-dependent molecular motors in immune cells. Journal of leukocyte biology 91, 35\u201346 (2012).","journal-title":"Journal of leukocyte biology"},{"key":"BFsrep25740_CR27","doi-asserted-by":"publisher","first-page":"2790","DOI":"10.1007\/s00018-008-8350-5","volume":"65","author":"S Loubery","year":"2008","unstructured":"Loubery, S. & Coudrier, E. Myosins in the secretory pathway: tethers or transporters? Cell Mol Life Sci 65, 2790\u20132800 (2008).","journal-title":"Cell Mol Life Sci"},{"key":"BFsrep25740_CR28","doi-asserted-by":"publisher","first-page":"153","DOI":"10.3389\/fendo.2013.00153","volume":"4","author":"A Papadopulos","year":"2013","unstructured":"Papadopulos, A., Tomatis, V. M., Kasula, R. & Meunier, F. A. The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane. Front Endocrinol (Lausanne) 4, 153 (2013).","journal-title":"Front Endocrinol (Lausanne)"},{"key":"BFsrep25740_CR29","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1007\/s00018-009-0215-z","volume":"67","author":"J Middelbeek","year":"2010","unstructured":"Middelbeek, J., Clark, K., Venselaar, H., Huynen, M. A. & van Leeuwen, F. N. The alpha-kinase family: an exceptional branch on the protein kinase tree. Cell Mol Life Sci 67, 875\u2013890 (2010).","journal-title":"Cell Mol Life Sci"},{"key":"BFsrep25740_CR30","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/S0014-5793(02)02299-8","volume":"514","author":"AG Ryazanov","year":"2002","unstructured":"Ryazanov, A. G. Elongation factor-2 kinase and its newly discovered relatives. FEBS Lett 514, 26\u201329 (2002).","journal-title":"FEBS Lett"},{"key":"BFsrep25740_CR31","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1023\/A:1024467426244","volume":"23","author":"MA De la Roche","year":"2002","unstructured":"De la Roche, M. A., Smith, J. L., Betapudi, V., Egelhoff, T. T. & Cote, G. P. Signaling pathways regulating Dictyostelium myosin II. J Muscle Res Cell Motil 23, 703\u2013718 (2002).","journal-title":"J Muscle Res Cell Motil"},{"key":"BFsrep25740_CR32","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1016\/j.jmb.2008.02.057","volume":"378","author":"K Clark","year":"2008","unstructured":"Clark, K. et al. TRPM7 regulates myosin IIA filament stability and protein localization by heavy chain phosphorylation. J Mol Biol 378, 790\u2013803 (2008).","journal-title":"J Mol Biol"},{"key":"BFsrep25740_CR33","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.tibs.2010.09.006","volume":"36","author":"SS Taylor","year":"2011","unstructured":"Taylor, S. S. & Kornev, A. P. Protein kinases: evolution of dynamic regulatory proteins. Trends Biochem Sci 36, 65\u201377 (2011).","journal-title":"Trends Biochem Sci"},{"key":"BFsrep25740_CR34","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1093\/eurheartj\/ehi879","volume":"27","author":"C Ruggiero","year":"2006","unstructured":"Ruggiero, C. et al. Uric acid and inflammatory markers. Eur Heart J 27, 1174\u20131181 (2006).","journal-title":"Eur Heart J"},{"key":"BFsrep25740_CR35","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1111\/j.1365-2249.2004.02563.x","volume":"137","author":"T McNearney","year":"2004","unstructured":"McNearney, T. et al. Excitatory amino acids, TNF-alpha, and chemokine levels in synovial fluids of patients with active arthropathies. Clin Exp Immunol 137, 621\u2013627 (2004).","journal-title":"Clin Exp Immunol"},{"key":"BFsrep25740_CR36","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1172\/JCI115142","volume":"87","author":"FS di Giovine","year":"1991","unstructured":"di Giovine, F. S., Malawista, S. E., Thornton, E. & Duff, G. W. Urate crystals stimulate production of tumor necrosis factor alpha from human blood monocytes and synovial cells. Cytokine mRNA and protein kinetics, and cellular distribution. J Clin Invest 87, 1375\u20131381 (1991).","journal-title":"J Clin Invest"},{"key":"BFsrep25740_CR37","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1136\/ard.2003.015743","volume":"63","author":"AK Tausche","year":"2004","unstructured":"Tausche, A. K. et al. Severe gouty arthritis refractory to anti-inflammatory drugs: treatment with anti-tumour necrosis factor alpha as a new therapeutic option. Annals of the rheumatic diseases 63, 1351\u20131352 (2004).","journal-title":"Annals of the rheumatic diseases"},{"key":"BFsrep25740_CR38","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1056\/NEJM199602153340707","volume":"334","author":"BT Emmerson","year":"1996","unstructured":"Emmerson, B. T. The management of gout. N Engl J Med. 334, 445\u2013451 (1996).","journal-title":"N Engl J Med."},{"key":"BFsrep25740_CR39","doi-asserted-by":"publisher","first-page":"407","DOI":"10.3109\/15563650.2010.495348","volume":"48","author":"Y Finkelstein","year":"2010","unstructured":"Finkelstein, Y. et al. Colchicine poisoning: the dark side of an ancient drug. Clin Toxicol (Phila) 48, 407\u2013414 (2010).","journal-title":"Clin Toxicol (Phila)"},{"key":"BFsrep25740_CR40","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1091\/mbc.e03-06-0440","volume":"15","author":"A Royou","year":"2004","unstructured":"Royou, A., Field, C., Sisson, J. C., Sullivan, W. & Karess, R. Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. Mol Biol Cell 15, 838\u2013850 (2004).","journal-title":"Mol Biol Cell"},{"key":"BFsrep25740_CR41","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1093\/bioinformatics\/16.4.404","volume":"16","author":"LJ McGuffin","year":"2000","unstructured":"McGuffin, L. J., Bryson, K. & Jones, D. T. The PSIPRED protein structure prediction server. Bioinformatics 16, 404\u2013405 (2000).","journal-title":"Bioinformatics"},{"key":"BFsrep25740_CR42","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/S0006-3495(67)86579-2","volume":"7","author":"M Schiffer","year":"1967","unstructured":"Schiffer, M. & Edmundson, A. B. Use of helical wheels to represent the structures of proteins and to identify segments with helical potential. Biophys J 7, 121\u2013135 (1967).","journal-title":"Biophys J"},{"key":"BFsrep25740_CR43","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1016\/j.str.2011.04.006","volume":"19","author":"S Mukherjee","year":"2011","unstructured":"Mukherjee, S. & Zhang, Y. Protein-protein complex structure predictions by multimeric threading and template recombination. Structure 19, 955\u2013966 (2011).","journal-title":"Structure"},{"key":"BFsrep25740_CR44","first-page":"755","volume":"15","author":"TO Crisan","year":"2015","unstructured":"Crisan, T. O. et al. Soluble uric acid primes TLR-induced proinflammatory cytokine production by human primary cells via inhibition of IL-1Ra. Annals of the rheumatic diseases 15, 755\u2013762 (2015).","journal-title":"Annals of the rheumatic diseases"},{"key":"BFsrep25740_CR45","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1124\/dmd.106.012187","volume":"35","author":"Z Yu","year":"2007","unstructured":"Yu, Z., Fong, W. P. & Cheng, C. H. Morin (3, 5, 7, 2\u2032, 4\u2032-pentahydroxyflavone) exhibits potent inhibitory actions on urate transport by the human urate anion transporter (hURAT1) expressed in human embryonic kidney cells. Drug metabolism and disposition: the biological fate of chemicals 35, 981\u2013986 (2007).","journal-title":"Drug metabolism and disposition: the biological fate of chemicals"},{"key":"BFsrep25740_CR46","doi-asserted-by":"crossref","first-page":"5631","DOI":"10.4049\/jimmunol.151.10.5631","volume":"151","author":"C Hwang","year":"1993","unstructured":"Hwang, C., Gatanaga, M., Granger, G. A. & Gatanaga, T. Mechanism of release of soluble forms of tumor necrosis factor\/lymphotoxin receptors by phorbol myristate acetate-stimulated human THP-1 cells in vitro . Journal of immunology 151, 5631\u20135638 (1993).","journal-title":"Journal of immunology"},{"key":"BFsrep25740_CR47","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1124\/jpet.109.152702","volume":"331","author":"T Takagi","year":"2009","unstructured":"Takagi, T. et al. Lansoprazole, a proton pump inhibitor, mediates anti-inflammatory effect in gastric mucosal cells through the induction of heme oxygenase-1 via activation of NF-E2-related factor 2 and oxidation of kelch-like ECH-associating protein 1. J Pharmacol Exp Ther 331, 255\u2013264 (2009).","journal-title":"J Pharmacol Exp Ther"},{"key":"BFsrep25740_CR48","doi-asserted-by":"publisher","first-page":"12564","DOI":"10.1073\/pnas.0914191107","volume":"107","author":"A Burgess","year":"2010","unstructured":"Burgess, A. et al. Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2\/PP2A balance. Proceedings of the National Academy of Sciences of the United States of America 107, 12564\u201312569 (2010).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"BFsrep25740_CR49","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1002\/art.1780200320","volume":"20","author":"SL Wallace","year":"1977","unstructured":"Wallace, S. L. et al. Preliminary criteria for the classification of the acute arthritis of primary gout. Arthritis Rheum 20, 895\u2013900 (1977).","journal-title":"Arthritis Rheum"}],"updated-by":[{"DOI":"10.1038\/srep27323","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000}}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep25740.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep25740","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep25740.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T06:20:32Z","timestamp":1672899632000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep25740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,12]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,5,27]]}},"alternative-id":["BFsrep25740"],"URL":"https:\/\/doi.org\/10.1038\/srep25740","relation":{"correction":[{"id-type":"doi","id":"10.1038\/srep27323","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,12]]},"assertion":[{"value":"10 August 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 April 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 May 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2016","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Update","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"25740"}}