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In order to elucidate Nczf functions, we generated Nczf knockout (Nczf\u2212\/\u2212) mice. Nczf\u2212\/\u2212 mice died around embryonic day 8.5 (E8.5) with small body size and impairment of axial rotation. Histopathological analysis revealed that the cell number decreased and pyknotic cells were occasionally observed. We examined the expression of cell cycle related genes in Nczf\u2212\/\u2212 mice. <jats:italic>p27<\/jats:italic> expression was increased in E8.0 Nczf\u2212\/\u2212 mice compared to that of wild type mice. Nczf knockdown by siRNA resulted in increased expression of <jats:italic>p27<\/jats:italic> in mouse embryonic fibroblasts (MEFs). Furthermore, <jats:italic>p27<\/jats:italic> promoter luciferase reporter gene analysis confirmed the regulation of <jats:italic>p27<\/jats:italic> mRNA expression by Nczf. Nczf\u2212\/\u2212; p27\u2212\/\u2212 double knockout mice survived until E11.5 and the defect of axial rotation was restored. These data suggest that <jats:italic>p27<\/jats:italic> repression by Nczf is essential in the developing embryo.<\/jats:p>","DOI":"10.1038\/srep26244","type":"journal-article","created":{"date-parts":[[2016,5,20]],"date-time":"2016-05-20T21:12:21Z","timestamp":1463778741000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Transcriptional repression of p27 is essential for murine embryonic development"],"prefix":"10.1038","volume":"6","author":[{"given":"Youichi","family":"Teratake","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisa","family":"Kuga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiharu","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayasu","family":"Kitahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruko","family":"Watanabe-Takano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akemi","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Arima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Tokuhisa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Hatano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,5,19]]},"reference":[{"key":"BFsrep26244_CR1","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1146\/annurev.cellbio.13.1.261","volume":"13","author":"DO Morgan","year":"1997","unstructured":"Morgan, D. 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