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NO is an important regulator of renal function, particularly, in the postnatal period. We hypothesized that the onset of renal dysfunction and hypertension in individuals with a SFK is associated with NO deficiency early in life. In this study, renal and cardiovascular responses to L-NAME infusion (N<jats:sup>w<\/jats:sup>-nitro-L-arginine methyl ester) were examined in 6-month old lambs born with a SFK, induced by fetal unilateral nephrectomy (uni-x). Renal responses to L-NAME were attenuated in uni-x sheep with the fall in glomerular filtration rate (GFR) and urinary sodium excretion (U<jats:sub>Na<\/jats:sub>V) being less in the uni-x compared to sham lambs (%\u0394GFR; \u221241\u2009\u00b1\u20093\u2009vs \u221254\u2009\u00b1\u20094: P\u2009=\u20090.03, %\u0394U<jats:sub>Na<\/jats:sub>V; \u221248\u2009\u00b1\u20095\u2009vs \u221276\u2009\u00b1\u20093, P\u2009=\u20090.0008). 24\u2009hour-basal urinary nitrate and nitrite (NOx) excretion was less in the uni-x animals compared to the sham (NOx excretion \u03bcM\/min\/kg; sham: 57\u2009\u00b1\u20097; uni-x: 38\u2009\u00b1\u20094, P\u2009=\u20090.02). L-NAME treatment reduced urinary NOx to undetectable levels in both groups. A reduction in NO bioavailability in early life may contribute to the initiation of glomerular and tubular dysfunction that promotes development and progression of hypertension in offspring with a congenital nephron deficit, including those with a SFK.<\/jats:p>","DOI":"10.1038\/srep26777","type":"journal-article","created":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T09:21:02Z","timestamp":1464254462000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Renal Nitric Oxide Deficiency and Chronic Kidney Disease in Young Sheep Born with a Solitary Functioning Kidney"],"prefix":"10.1038","volume":"6","author":[{"given":"Reetu R.","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence K.","family":"Easton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsea C.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus P.","family":"Schlaich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey A.","family":"Head","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen M.","family":"Moritz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate M.","family":"Denton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,5,26]]},"reference":[{"key":"BFsrep26777_CR1","doi-asserted-by":"publisher","first-page":"e478","DOI":"10.1542\/peds.2012-2088","volume":"131","author":"R Westland","year":"2013","unstructured":"Westland, R., Kurvers, R. 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