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The precipitate was resuspended in the same volume of milk. The immunoblot analysis of the suspended proteins treated with 200\u2009ng of trypsin\/ml for 3\u2009h demonstrated that the 80.1% of the insulin in the suspension survived the proteolytic degradation compared to 0% of the hormone survived in the control. The feeding of 0.4\u2009ml (0.08 unit of insulin) of the resuspended proteins followed by 0.2\u2009ml of the same protein to alloxan induced diabetic mice maximally decreased the blood glucose level from 508\u2009\u00b1\u200910\u2009mg\/dl to 130\u2009\u00b1\u200910\u2009mg\/dl in 7\u2009h with simultaneous increase of the basal plasma concentration of insulin from 3\u2009\u00b1\u20091.1 \u03bcunits\/ml to 18\u2009\u00b1\u20091.5 \u03bcunits\/ml. In control experiment the absence of insulin in the identical milk suspension produced no hypoglycemic effect suggesting milk was not responsible for the hypoglycemic effect of milk-insulin complex. Coming out of insulin-casein complex from the intestinal gut to the circulation was spontaneous and facilitated diffusion transportation which was found from Gibbs free energy reaction.<\/jats:p>","DOI":"10.1038\/srep26789","type":"journal-article","created":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T09:08:42Z","timestamp":1464253722000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The control of hyperglycemia by a novel trypsin resistant oral insulin preparation in alloxan induced type I diabetic mice"],"prefix":"10.1038","volume":"6","author":[{"given":"Sarbashri","family":"Bank","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjun","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suman","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smarajit","family":"Maiti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gausal A.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asru K","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,5,26]]},"reference":[{"key":"BFsrep26789_CR1","first-page":"251","volume":"7","author":"FG Banting","year":"1922","unstructured":"Banting, F. 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