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The trimethylsulfonium ion (TMS) can result from successive methylation reactions of hydrogen sulfide. No report exists so far about the presence or quantities of TMS in human urine. We developed a method for determining TMS in urine using liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry (LC-ESI-QQQ) and applied the method to establish the urinary levels of TMS in a group of human volunteers. The measured urinary levels of TMS were in the nanomolar range, which is commensurate with the steady-state tissue concentrations of hydrogen sulfide previously reported in the literature. The developed method can be used in future studies for the quantification of urinary TMS as a potential biomarker for hydrogen sulfide body pools.<\/jats:p>","DOI":"10.1038\/srep27038","type":"journal-article","created":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T09:59:57Z","timestamp":1464775197000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The hydrogen sulfide metabolite trimethylsulfonium is found in human urine"],"prefix":"10.1038","volume":"6","author":[{"given":"Bassam","family":"Lajin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin A.","family":"Francesconi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,1]]},"reference":[{"key":"BFsrep27038_CR1","doi-asserted-by":"publisher","first-page":"1641S","DOI":"10.1093\/jn\/136.6.1641S","volume":"136","author":"Y Ingenbleek","year":"2006","unstructured":"Ingenbleek, Y. 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