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Despite its biological relevance, the structural nature of the membrane-bound state \u03b1S remains elusive, in part because of the intrinsically dynamic nature of the protein and also because of the difficulties in studying this state in a physiologically relevant environment. In the present study we have used solid-state NMR and restrained MD simulations to refine structure and topology of the N-terminal region of \u03b1S bound to the surface of synaptic-like membranes. This region has fundamental importance in the binding mechanism of \u03b1S as it acts as to anchor the protein to lipid bilayers. The results enabled the identification of the key elements for the biological properties of \u03b1S in its membrane-bound state.<\/jats:p>","DOI":"10.1038\/srep27125","type":"journal-article","created":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T05:50:33Z","timestamp":1465365033000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["Structural Ensembles of Membrane-bound \u03b1-Synuclein Reveal the Molecular Determinants of Synaptic Vesicle Affinity"],"prefix":"10.1038","volume":"6","author":[{"given":"Giuliana","family":"Fusco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"De Simone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Arosio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Vendruscolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluigi","family":"Veglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Dobson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"BFsrep27125_CR1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1126\/science.1227157","volume":"338","author":"KC Luk","year":"2012","unstructured":"Luk, K. 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