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Taking advantage of recent developments in the genetic transformation in <jats:italic>C. trachomatis<\/jats:italic>, we constructed a versatile green fluorescent protein (GFP) reporter system to study the development-dependent function of <jats:italic>C. trachomatis<\/jats:italic> promoters in an attempt to elucidate the mechanism that controls <jats:italic>C. trachomatis<\/jats:italic> adaptability. We validated the use of the GFP reporter system by visualizing the activity of an early <jats:italic>euo<\/jats:italic> gene promoter. Additionally, we uncovered a new <jats:italic>ompA<\/jats:italic> promoter, which we named P3, utilizing the GFP reporter system combined with 5\u2032 rapid amplification of cDNA ends (RACE), <jats:italic>in vitro<\/jats:italic> transcription assays, real-time quantitative RT-PCR (RT-qPCR) and flow cytometry. Mutagenesis of the P3 region verifies that P3 is a new class of <jats:italic>C. trachomatis<\/jats:italic> \u03c3<jats:sup>66<\/jats:sup>-dependent promoter, which requires an extended \u221210 TGn motif for transcription. These results corroborate complex developmentally controlled <jats:italic>ompA<\/jats:italic> expression in <jats:italic>C. trachomatis.<\/jats:italic> The exploitation of genetically labeled <jats:italic>C. trachomatis<\/jats:italic> organisms with P3-driven GFP allows for the observation of changes in <jats:italic>ompA<\/jats:italic> expression in response to developmental signals. The results of this study could be used to complement previous findings and to advance understanding of <jats:italic>C. trachomatis<\/jats:italic> genetic expression.<\/jats:p>","DOI":"10.1038\/srep27244","type":"journal-article","created":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T05:46:14Z","timestamp":1465191974000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis"],"prefix":"10.1038","volume":"6","author":[{"given":"Yanguang","family":"Cong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leiqiong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Xian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyu","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiba","family":"Elaasar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,6,6]]},"reference":[{"key":"BFsrep27244_CR1","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1093\/infdis\/jis644","volume":"207","author":"ML Rekart","year":"2013","unstructured":"Rekart, M. 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