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However, advancing age exerts detrimental effects upon gametes which can have serious consequences upon embryo viability. While such effects are well documented for the oocyte, relatively little is known with regard to the sperm. One fundamental role of sperm is to activate the oocyte at fertilisation, a process initiated by phospholipase C zeta (PLC\u03b6), a sperm-specific protein. While PLC\u03b6 deficiency can lead to oocyte activation deficiency and infertility, it is currently unknown whether the expression or function of PLC\u03b6 is compromised by advancing male age. Here, we evaluate sperm motility and the proportion of sperm expressing PLC\u03b6 in 71 males (22\u201354 years; 44 fertile controls and 27 infertile patients), along with total levels and localisation patterns of PLC\u03b6 within the sperm head. Three different statistical approaches were deployed with male age considered both as a categorical and a continuous factor. While progressive motility was negatively correlated with male age, all three statistical models concurred that no PLC\u03b6\u2013related parameter was associated with male age, suggesting that advancing male age is unlikely to cause problems in terms of the sperm\u2019s fundamental ability to activate an oocyte.<\/jats:p>","DOI":"10.1038\/srep27543","type":"journal-article","created":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T09:42:19Z","timestamp":1465378939000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Does advancing male age influence the expression levels and localisation patterns of phospholipase C zeta (PLC\u03b6) in human sperm?"],"prefix":"10.1038","volume":"6","author":[{"given":"Marc","family":"Yeste","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celine","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siti Nornadhirah","family":"Amdani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suseela","family":"Yelumalai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ginny","family":"Mounce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah J. Martins","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Child","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Coward","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"BFsrep27543_CR1","doi-asserted-by":"crossref","unstructured":"Practice Committee of the American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss: a committee opinion. Fertil. Steril. 99, 63 (2013).","DOI":"10.1016\/j.fertnstert.2012.09.023"},{"key":"BFsrep27543_CR2","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1016\/j.fertnstert.2009.09.009","volume":"92","author":"F Zegers-Hochschild","year":"2009","unstructured":"Zegers-Hochschild, F. et al. International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil. Steril. 92, 1520\u20131524 (2009).","journal-title":"Fertil. Steril."},{"key":"BFsrep27543_CR3","doi-asserted-by":"crossref","unstructured":"Yeste, M., Jones, C., Amdani, S. N., Patel, S. & Coward, K. Oocyte activation deficiency: a role for an oocyte contribution? Hum. Reprod. Update, 10.1093\/humupd\/dmv040 (2015).","DOI":"10.1093\/humupd\/dmv040"},{"key":"BFsrep27543_CR4","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1038\/nm.f.1895","volume":"14","author":"MM Matzuk","year":"2008","unstructured":"Matzuk, M. M. & Lamb, D. J. The biology of infertility: research advances and clinical challenges. Nat. Med. 14, 1197\u20131213 (2008).","journal-title":"Nat. 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