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In the present study, we investigated the relationship between cell death and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-\/CCl<jats:sub>4<\/jats:sub>-induced LMP in hepatocytes <jats:italic>in vitro<\/jats:italic> and following acute liver injury <jats:italic>in vivo<\/jats:italic>. The key finding was that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> triggered LMP by oxidative stress, as evidenced by a suppression of LAMP1 expression, a reduction in LysoTracker Green and AO staining and the leakage of proton and cathepsin B\/D from the lysosome to the cytoplasm, resulting in cell death. CCl<jats:sub>4<\/jats:sub> also triggered hepatocyte death by decreasing lysosome LAMP1 expression and by inducing the accumulation of products of peroxidative lipids and oxidized proteins. Furthermore, a novel compound 5,8-dimethoxy-6-methyl-7-hydroxy-3-3(2-hydroxy-4-methoxybenzyl) chroman-4-one (58-F) was extracted from <jats:italic>Ophiopogon japonicus<\/jats:italic> and served as a potential therapeutic drug. <jats:italic>In vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> results showed that 58-F effectively rescued hepatocytes by decreasing LMP and by inducing lysosomal enzyme translocation to the cytosol.<\/jats:p>","DOI":"10.1038\/srep27875","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T09:48:27Z","timestamp":1466070507000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["58-F, a flavanone from Ophiopogon japonicus, prevents hepatocyte death by decreasing lysosomal membrane permeability"],"prefix":"10.1038","volume":"6","author":[{"given":"Xiaofeng","family":"Yan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingjie","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,16]]},"reference":[{"key":"BFsrep27875_CR1","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.bbapap.2011.10.002","volume":"1824","author":"V Turk","year":"2012","unstructured":"Turk, V. et al. 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