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It is transported from the cell body into dendrites where it is involved in the fine-tuning translational control. Due to its compactness and established secondary structure, BC1 RNA is an ideal model for investigating the motifs necessary for dendritic localization. Previously, microinjection of <jats:italic>in vitro<\/jats:italic> transcribed BC1 RNA mutants into the soma of cultured primary neurons suggested the importance of RNA motifs for dendritic targeting. These <jats:italic>ex vivo<\/jats:italic> experiments identified a single bulged nucleotide (U<jats:sub>22<\/jats:sub>) and a putative K-turn (GA motif) structure required for dendritic localization or distal transport, respectively. We generated six transgenic mouse lines (three founders each) containing neuronally expressing BC1 RNA variants on a BC1 RNA knockout mouse background. In contrast to <jats:italic>ex vivo<\/jats:italic> data, we did not find indications of reduction or abolition of dendritic BC1 RNA localization in the mutants devoid of the GA motif or the bulged nucleotide. We confirmed the <jats:italic>ex vivo<\/jats:italic> data, which showed that the triloop terminal sequence had no consequence on dendritic transport. Interestingly, changing the triloop supporting structure completely abolished dendritic localization of BC1 RNA. We propose a novel RNA motif important for dendritic transport <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/srep28300","type":"journal-article","created":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T09:19:11Z","timestamp":1467105551000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["BC1 RNA motifs required for dendritic transport in vivo"],"prefix":"10.1038","volume":"6","author":[{"given":"Thomas","family":"Robeck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris V.","family":"Skryabin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timofey S.","family":"Rozhdestvensky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasiya B.","family":"Skryabin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Brosius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,6,28]]},"reference":[{"key":"BFsrep28300_CR1","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1080\/15476286.2015.1128064","volume":"13","author":"J Brosius","year":"2016","unstructured":"Brosius, J. & Raabe, C. 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