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The results showed that UV-B irradiation significantly increased the anthocyanin accumulation and the expression of <jats:italic>UVR8<\/jats:italic>, and a similar trend appeared in radish sprouts subjected to cadmium, chilling and salt stresses regardless of light source. However, these responses disappeared under dark exposure. These results suggest that abiotic stress-induced anthocyanin accumulation and <jats:italic>UVR8<\/jats:italic> expression were light-dependent. Moreover, abiotic stresses all enhanced the production of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and exogenous H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> addition significantly increased the anthocyanin concentration and <jats:italic>UVR8<\/jats:italic> transcription, while these increases were severely inhibited by addition of dimethylthiourea (DMTU, a chemical trap for H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>). It seems to suggest that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> played an important role in the anthocyanin biosynthesis. Furthermore, addition of 0.5\u2009mM sodium nitroprusside (SNP, a NO-releasing compound) substantially induced the anthocyanin accumulation, and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-induced anthocyanin accumulation and <jats:italic>UVR8<\/jats:italic> expression were significantly suppressed by co-treatment with 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO, a NO scavenger), which was parallel with the expression of anthocyanin biosynthesis-related transcription factors and structural genes. All these results demonstrate that both H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and NO are involved in UV-B-induced anthocyanin accumulation, and there is a crosstalk between them as well as a classical UVR8 pathway.<\/jats:p>","DOI":"10.1038\/srep29164","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T12:48:21Z","timestamp":1468327701000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Hydrogen peroxide, nitric oxide and UV RESISTANCE LOCUS8 interact to mediate UV-B-induced anthocyanin biosynthesis in radish sprouts"],"prefix":"10.1038","volume":"6","author":[{"given":"Qi","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongchao","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,12]]},"reference":[{"key":"BFsrep29164_CR1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1104\/pp.114.253674","volume":"167","author":"K Yoshida","year":"2015","unstructured":"Yoshida, K., Ma, D. 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