{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:16:46Z","timestamp":1791083806686,"version":"4.1.0"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T00:00:00Z","timestamp":1468454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T00:00:00Z","timestamp":1468454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>H<jats:sub>2<\/jats:sub>S is an important signalling molecule involved in diverse biological processes. It mediates the formation of cysteine persulfides (R-S-SH), which affect the activity of target proteins. Like thiols, persulfides show reactivity towards electrophiles and behave similarly to other cysteine modifications in a biotin switch assay. In this manuscript, we report on qPerS-SID a mass spectrometry-based method allowing the isolation of persulfide containing peptides in the mammalian proteome. With this method, we demonstrated that H<jats:sub>2<\/jats:sub>S donors differ in their efficacy to induce persulfides in HEK293 cells. Furthermore, data analysis revealed that persulfide formation affects all subcellular compartments and various cellular processes. Negatively charged amino acids appeared more frequently adjacent to cysteines forming persulfides. We confirmed our proteomic data using pyruvate kinase M2 as a model protein and showed that several cysteine residues are prone to persulfide formation finally leading to its inactivation. Taken together, the site-specific identification of persulfides on a proteome scale can help to identify target proteins involved in H<jats:sub>2<\/jats:sub>S signalling and enlightens the biology of H<jats:sub>2<\/jats:sub>S and its releasing agents.<\/jats:p>","DOI":"10.1038\/srep29808","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T11:09:44Z","timestamp":1468494584000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Quantitative Persulfide Site Identification (qPerS-SID) Reveals Protein Targets of H2S Releasing Donors in Mammalian Cells"],"prefix":"10.1038","volume":"6","author":[{"given":"Sebastian","family":"Longen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette","family":"K\u00f6hler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka","family":"Wittig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl-Friedrich","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Pfeilschifter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,14]]},"reference":[{"key":"BFsrep29808_CR1","doi-asserted-by":"publisher","first-page":"4633","DOI":"10.1021\/cr300163e","volume":"113","author":"CE Paulsen","year":"2013","unstructured":"Paulsen, C. E. & Carroll, K. S. Cysteine-mediated redox signaling: Chemistry, biology and tools for discovery. Chem. Rev. 113, 4633\u20134679 (2013).","journal-title":"Chem. Rev."},{"key":"BFsrep29808_CR2","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1016\/j.pharep.2015.01.005","volume":"67","author":"J Be\u0142towski","year":"2015","unstructured":"Be\u0142towski, J. Hydrogen sulfide in pharmacology and medicine - An update. Pharmacol. Rep. 67, 647\u2013658 (2015).","journal-title":"Pharmacol. Rep."},{"key":"BFsrep29808_CR3","doi-asserted-by":"publisher","first-page":"16146","DOI":"10.3390\/molecules191016146","volume":"19","author":"H Kimura","year":"2014","unstructured":"Kimura, H. Hydrogen Sulfide and Polysulfides as Biological Mediators. Molecules 19, 16146\u201316157 (2014).","journal-title":"Molecules"},{"key":"BFsrep29808_CR4","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1089\/ars.2014.6082","volume":"22","author":"H Kimura","year":"2015","unstructured":"Kimura, H. Signaling of Hydrogen Sulfide and Polysulfides. Antioxid. Redox Signal. 22, 347\u2013349 (2015).","journal-title":"Antioxid. Redox Signal."},{"key":"BFsrep29808_CR5","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1161\/CIRCULATIONAHA.107.753467","volume":"117","author":"L Li","year":"2008","unstructured":"Li, L. et al. Characterization of a novel, water-soluble hydrogen sulfide-releasing molecule (GYY4137): New insights into the biology of hydrogen sulfide. Circulation 117, 2351\u20132360 (2008).","journal-title":"Circulation"},{"key":"BFsrep29808_CR6","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/nchembio.1834","volume":"11","author":"TV Mishanina","year":"2015","unstructured":"Mishanina, T. V., Libiad, M. & Banerjee, R. Biogenesis of reactive sulfur species for signaling by hydrogen sulfide oxidation pathways. Nat. Chem. Biol. 11, 457\u2013464 (2015).","journal-title":"Nat. Chem. Biol."},{"key":"BFsrep29808_CR7","doi-asserted-by":"publisher","first-page":"2451","DOI":"10.1096\/fj.12-226415","volume":"27","author":"Y Kimura","year":"2013","unstructured":"Kimura, Y. et al. Polysulfides are possible H2S-derived signaling molecules in rat brain. FASEB J. 27, 2451\u20132457 (2013).","journal-title":"FASEB J."},{"key":"BFsrep29808_CR8","doi-asserted-by":"publisher","first-page":"14774","DOI":"10.1038\/srep14774","volume":"5","author":"Y Kimura","year":"2015","unstructured":"Kimura, Y. et al. Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. Sci. Rep. 5, 14774 (2015).","journal-title":"Sci. Rep."},{"key":"BFsrep29808_CR9","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1089\/ars.2012.5041","volume":"19","author":"R Greiner","year":"2013","unstructured":"Greiner, R. et al. Polysulfides link H2S to protein thiol oxidation. Antioxid. Redox Signal. 19, 1749\u20131765 (2013).","journal-title":"Antioxid. Redox Signal."},{"key":"BFsrep29808_CR10","doi-asserted-by":"crossref","unstructured":"Mustafa, A. K. et al. H2S signals through protein S-sulfhydration. Sci. Signal 2, ra72 (2009).","DOI":"10.1126\/scisignal.2000464"},{"key":"BFsrep29808_CR11","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.1038\/ncomms2623","volume":"4","author":"MS Vandiver","year":"2013","unstructured":"Vandiver, M. S. et al. Sulfhydration mediates neuroprotective actions of parkin. Nat. Commun. 4, 1626 (2013).","journal-title":"Nat. Commun."},{"key":"BFsrep29808_CR12","doi-asserted-by":"publisher","first-page":"1906","DOI":"10.1089\/ars.2012.4645","volume":"18","author":"G Yang","year":"2013","unstructured":"Yang, G. et al. Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2. Antioxid. Redox Signal. 18, 1906\u20131919 (2013).","journal-title":"Antioxid. Redox Signal."},{"key":"BFsrep29808_CR13","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1002\/embr.201338271","volume":"15","author":"K Zhao","year":"2014","unstructured":"Zhao, K. et al. S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO Rep. 15, 792\u2013800 (2014).","journal-title":"EMBO Rep."},{"key":"BFsrep29808_CR14","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.molcel.2011.10.021","volume":"45","author":"N Sen","year":"2012","unstructured":"Sen, N. et al. Hydrogen sulfide-linked sulfhydration of NF-\u03baB mediates its antiapoptotic actions. Mol. Cell 45, 13\u201324 (2012).","journal-title":"Mol. Cell"},{"key":"BFsrep29808_CR15","doi-asserted-by":"publisher","first-page":"ra86","DOI":"10.1126\/scisignal.2002329","volume":"4","author":"N Krishnan","year":"2011","unstructured":"Krishnan, N., Fu, C., Pappin, D. J. & Tonks, N. K. H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response. Sci Signal 4, ra86 (2011).","journal-title":"Sci Signal"},{"key":"BFsrep29808_CR16","doi-asserted-by":"publisher","first-page":"1259","DOI":"10.1161\/CIRCRESAHA.111.240242","volume":"109","author":"AK Mustafa","year":"2011","unstructured":"Mustafa, A. K. et al. Hydrogen sulfide as endothelium-derived hyperpolarizing factor sulfhydrates potassium channels. Circ. Res. 109, 1259\u20131268 (2011).","journal-title":"Circ. Res."},{"key":"BFsrep29808_CR17","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.stem.2014.03.005","volume":"15","author":"Y Liu","year":"2014","unstructured":"Liu, Y. et al. Hydrogen sulfide maintains mesenchymal stem cell function and bone homeostasis via regulation of Ca2+ channel sulfhydration. Cell Stem Cell 15, 66\u201378 (2014).","journal-title":"Cell Stem Cell"},{"key":"BFsrep29808_CR18","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1002\/anie.201305876","volume":"53","author":"D Zhang","year":"2014","unstructured":"Zhang, D. et al. Detection of protein S-sulfhydration by a tag-switch technique. Angew. Chemie\u2013Int. Ed. 53, 575\u2013581 (2014).","journal-title":"Angew. Chemie\u2013Int. Ed."},{"key":"BFsrep29808_CR19","doi-asserted-by":"publisher","first-page":"7606","DOI":"10.1073\/pnas.1321232111","volume":"111","author":"T Ida","year":"2014","unstructured":"Ida, T. et al. Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling. Proc. Natl. Acad. Sci. 111, 7606\u20137611 (2014).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"BFsrep29808_CR20","doi-asserted-by":"publisher","first-page":"e10067","DOI":"10.7554\/eLife.10067","volume":"4","author":"X-H Gao","year":"2015","unstructured":"Gao, X.-H. et al. Quantitative H2S-mediated protein sulfhydration reveals metabolic reprogramming during the Integrated Stress Response. Elife 4, e10067 (2015).","journal-title":"Elife"},{"key":"BFsrep29808_CR21","doi-asserted-by":"publisher","first-page":"e1500968","DOI":"10.1126\/sciadv.1500968","volume":"2","author":"\u00c9 D\u00f3ka","year":"2016","unstructured":"D\u00f3ka, \u00c9. et al. A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems. Sci. Adv. 2(1), e1500968 (2016)","journal-title":"Sci. Adv."},{"key":"BFsrep29808_CR22","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.1093\/oxfordjournals.molbev.a026406","volume":"17","author":"A Miseta","year":"2000","unstructured":"Miseta, A. & Csutora, P. Relationship between the occurrence of cysteine in proteins and the complexity of organisms. Mol. Biol. Evol. 17, 1232\u20131239 (2000).","journal-title":"Mol. Biol. Evol."},{"key":"BFsrep29808_CR23","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1073\/pnas.0812149106","volume":"106","author":"RE Hansen","year":"2009","unstructured":"Hansen, R. E., Roth, D. & Winther, J. R. Quantifying the global cellular thiol-disulfide status. Proc. Natl. Acad. Sci. USA 106, 422\u2013427 (2009).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep29808_CR24","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1021\/cb4001052","volume":"8","author":"J Pan","year":"2013","unstructured":"Pan, J. & Carroll, K. S. Persulfide reactivity in the detection of protein S-sulfhydration. ACS Chem. Biol. 8, 1110\u20131116 (2013).","journal-title":"ACS Chem. Biol."},{"key":"BFsrep29808_CR25","doi-asserted-by":"publisher","first-page":"2337","DOI":"10.1002\/rcm.1196","volume":"17","author":"B Ma","year":"2003","unstructured":"Ma, B. et al. PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry. Rapid Commun. Mass Spectrom. 17, 2337\u20132342 (2003).","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"BFsrep29808_CR26","doi-asserted-by":"publisher","first-page":"3576","DOI":"10.1021\/ac0103423","volume":"73","author":"ES Boja","year":"2001","unstructured":"Boja, E. S. & Fales, H. M. Overalkylation of a protein digest with iodoacetamide. Anal. Chem. 73, 3576\u20133582 (2001).","journal-title":"Anal. Chem."},{"key":"BFsrep29808_CR27","doi-asserted-by":"publisher","first-page":"6150","DOI":"10.1111\/febs.12535","volume":"280","author":"Ja Reisz","year":"2013","unstructured":"Reisz, J. a., Bechtold, E., King, S. B., Poole, L. B. & Furdui, C. M. Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J. 280, 6150\u20136161 (2013).","journal-title":"FEBS J."},{"key":"BFsrep29808_CR28","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nbt.1511","volume":"26","author":"J Cox","year":"2008","unstructured":"Cox, J. & Mann, M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat. Biotechnol. 26, 1367\u20131372 (2008).","journal-title":"Nat. Biotechnol."},{"key":"BFsrep29808_CR29","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1038\/nmeth.2646","volume":"10","author":"JP O\u2019Shea","year":"2013","unstructured":"O\u2019Shea, J. P. et al. pLogo: a probabilistic approach to visualizing sequence motifs. Nat. Methods 10, 1211\u20131212 (2013).","journal-title":"Nat. Methods"},{"key":"BFsrep29808_CR30","doi-asserted-by":"publisher","first-page":"4776","DOI":"10.1038\/ncomms5776","volume":"5","author":"J Yang","year":"2014","unstructured":"Yang, J., Gupta, V., Carroll, K. S. & Liebler, D. C. Site-specific mapping and quantification of protein S-sulphenylation in cells. Nat. Commun. 5, 4776 (2014).","journal-title":"Nat. Commun."},{"key":"BFsrep29808_CR31","doi-asserted-by":"crossref","unstructured":"Doulias, P.-T., Tenopoulou, M., Greene, J. L., Raju, K. & Ischiropoulos, H. Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation. Sci. Signal 6, rs1 (2013).","DOI":"10.1126\/scisignal.2003252"},{"key":"BFsrep29808_CR32","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1021\/tx2005234","volume":"25","author":"JM Fukuto","year":"2012","unstructured":"Fukuto, J. M. et al. Small molecule signaling agents: The integrated chemistry and biochemistry of nitrogen oxides, oxides of carbon, dioxygen, hydrogen sulfide and their derived species. Chem. Res. Toxicol. 25, 769\u2013793 (2012).","journal-title":"Chem. Res. Toxicol."},{"key":"BFsrep29808_CR33","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1110\/ps.03576904","volume":"13","author":"MD de Beus","year":"2004","unstructured":"de Beus, M. D., Chung, J. & Col\u00f3n, W. Modification of cysteine 111 in Cu\/Zn superoxide dismutase results in altered spectroscopic and biophysical properties. Protein Sci. 13, 1347\u20131355 (2004).","journal-title":"Protein Sci."},{"key":"BFsrep29808_CR34","doi-asserted-by":"publisher","first-page":"1851","DOI":"10.1126\/science.281.5384.1851","volume":"281","author":"LI Bruijn","year":"1998","unstructured":"Bruijn, L. I. et al. Aggregation and motor neuron toxicity of an ALS-linked SOD1 mutant independent from wild-type SOD1. Science 281, 1851\u20131854 (1998).","journal-title":"Science"},{"key":"BFsrep29808_CR35","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1016\/j.freeradbiomed.2015.09.007","volume":"89","author":"AP Jarosz","year":"2015","unstructured":"Jarosz, A. P. et al. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is inactivated by S-sulfuration in vitro. Free Radic. Biol. Med. 89, 512\u2013521 (2015).","journal-title":"Free Radic. Biol. Med."},{"key":"BFsrep29808_CR36","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/nature06734","volume":"452","author":"HR Christofk","year":"2008","unstructured":"Christofk, H. R. et al. The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature 452, 230\u2013233 (2008).","journal-title":"Nature"},{"key":"BFsrep29808_CR37","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.1126\/science.1211485","volume":"334","author":"D Anastasiou","year":"2011","unstructured":"Anastasiou, D. et al. Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Science 334, 1278\u20131283 (2011).","journal-title":"Science"},{"key":"BFsrep29808_CR38","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1042\/bj1310223","volume":"131","author":"S Ainsworth","year":"1973","unstructured":"Ainsworth, S. & MacFarlane, N. A kinetic study of rabbit muscle pyruvate kinase. Biochem. J. 131, 223\u2013236 (1973).","journal-title":"Biochem. J."},{"key":"BFsrep29808_CR39","doi-asserted-by":"publisher","first-page":"013441","DOI":"10.1074\/mcp.M111.013441","volume":"11","author":"CI Murray","year":"2012","unstructured":"Murray, C. I., Uhrigshardt, H., O\u2019Meally, R. N., Cole, R. N. & Van Eyk, J. E. Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay. Mol. Cell. Proteomics 11, M111.013441 (2012).","journal-title":"Mol. Cell. Proteomics"},{"key":"BFsrep29808_CR40","doi-asserted-by":"crossref","unstructured":"Jaffrey, S. R. & Snyder, S. H. The biotin switch method for the detection of S-nitrosylated proteins. Sci. STKE 2001, pl1 (2001).","DOI":"10.1126\/scisignal.862001pl1"},{"key":"BFsrep29808_CR41","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/S0076-6879(10)74017-9","volume":"474","author":"SW Aesif","year":"2010","unstructured":"Aesif, S. W., Janssen-Heininger, Y. M. W. & Reynaert, N. L. Protocols for the detection of s-glutathionylated and s-nitrosylated proteins in situ. Methods Enzymol. 474, 289\u2013296 (2010).","journal-title":"Methods Enzymol."},{"key":"BFsrep29808_CR42","doi-asserted-by":"crossref","unstructured":"Lee, Z. W. et al. Utilizing hydrogen sulfide as a novel anti-cancer agent by targeting cancer glycolysis and pH imbalance. Br. J. Pharmacol. doi: 10.1111\/bph.12773 (2014).","DOI":"10.1111\/bph.12773"},{"key":"BFsrep29808_CR43","doi-asserted-by":"publisher","first-page":"H1215","DOI":"10.1152\/ajpheart.00796.2012","volume":"304","author":"BF Peake","year":"2013","unstructured":"Peake, B. F. et al. Hydrogen sulfide preconditions the db\/db diabetic mouse heart against ischemia-reperfusion injury by activating Nrf2 signaling in an Erk-dependent manner. Am. J. Physiol. Heart Circ. Physiol. 304, H1215\u2013H1224 (2013).","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"BFsrep29808_CR44","doi-asserted-by":"publisher","first-page":"30667","DOI":"10.1074\/jbc.M705929200","volume":"282","author":"ZT Kelleher","year":"2007","unstructured":"Kelleher, Z. T., Matsumoto, A., Stamler, J. S. & Marshall, H. E. NOS2 regulation of NF-kappaB by S-nitrosylation of p65. J Biol Chem. 282, 30667\u201330672 (2007).","journal-title":"J Biol Chem."},{"key":"BFsrep29808_CR45","doi-asserted-by":"publisher","first-page":"2236","DOI":"10.1093\/nar\/24.12.2236","volume":"24","author":"JR Matthews","year":"1996","unstructured":"Matthews, J. R., Botting, C. H., Panico, M., Morris, H. R. & Hay, R. T. Inhibition of NF-kappaB DNA binding by nitric oxide. Nucleic Acids Res. 24, 2236\u20132242 (1996).","journal-title":"Nucleic Acids Res."},{"key":"BFsrep29808_CR46","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/nchembio720","volume":"1","author":"Da Mitchell","year":"2005","unstructured":"Mitchell, D. a. & Marletta, M. a. Thioredoxin catalyzes the S-nitrosation of the caspase-3 active site cysteine. Nat. Chem. Biol. 1, 154\u2013158 (2005).","journal-title":"Nat. Chem. Biol."},{"key":"BFsrep29808_CR47","doi-asserted-by":"publisher","first-page":"11609","DOI":"10.1073\/pnas.0704898104","volume":"104","author":"Da Mitchell","year":"2007","unstructured":"Mitchell, D. a., Morton, S. U., Fernhoff, N. B. & Marletta, M. a. Thioredoxin is required for S-nitrosation of procaspase-3 and the inhibition of apoptosis in Jurkat cells. Proc. Natl. Acad. Sci. USA 104, 11609\u201311614 (2007).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep29808_CR48","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.ejcts.2008.03.017","volume":"34","author":"M Bliks\u00f8en","year":"2008","unstructured":"Bliks\u00f8en, M., Kaljusto, M.-L., Vaage, J. & Stensl\u00f8kken, K.-O. Effects of hydrogen sulphide on ischaemia-reperfusion injury and ischaemic preconditioning in the isolated, perfused rat heart. Eur. J. Cardiothorac. Surg. 34, 344\u2013349 (2008).","journal-title":"Eur. J. Cardiothorac. Surg."},{"key":"BFsrep29808_CR49","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.ejphar.2009.01.041","volume":"606","author":"P Tripatara","year":"2009","unstructured":"Tripatara, P. et al. Characterisation of cystathionine gamma-lyase\/hydrogen sulphide pathway in ischaemia\/reperfusion injury of the mouse kidney: an in vivo study. Eur. J. Pharmacol. 606, 205\u2013209 (2009).","journal-title":"Eur. J. Pharmacol."},{"key":"BFsrep29808_CR50","doi-asserted-by":"publisher","first-page":"15560","DOI":"10.1073\/pnas.0705891104","volume":"104","author":"JW Elrod","year":"2007","unstructured":"Elrod, J. W. et al. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function. Proc. Natl. Acad. Sci. USA 104, 15560\u201315565 (2007).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep29808_CR51","doi-asserted-by":"publisher","first-page":"12474","DOI":"10.1073\/pnas.1306241110","volume":"110","author":"C Szabo","year":"2013","unstructured":"Szabo, C. et al. Tumor-derived hydrogen sulfide, produced by cystathionine-\u03b2-synthase, stimulates bioenergetics, cell proliferation and angiogenesis in colon cancer. Proc. Natl. Acad. Sci. USA 110, 12474\u201312479 (2013).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep29808_CR52","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1089\/ars.2014.5933","volume":"22","author":"MR Hellmich","year":"2014","unstructured":"Hellmich, M. R., Coletta, C., Chao, C. & Szabo, C. The Therapeutic Potential of Cystathionine \u03b2-Synthetase\/Hydrogen Sulfide Inhibition in Cancer. Antioxid. Redox Signal. 22, 424\u2013448 (2014).","journal-title":"Antioxid. Redox Signal."},{"key":"BFsrep29808_CR53","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1111\/j.1474-9726.2009.00543.x","volume":"9","author":"L-F Hu","year":"2010","unstructured":"Hu, L.-F. et al. Neuroprotective effects of hydrogen sulfide on Parkinson\u2019s disease rat models. Aging Cell 9, 135\u2013146 (2010).","journal-title":"Aging Cell"},{"key":"BFsrep29808_CR54","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1089\/ars.2010.3671","volume":"15","author":"K Kida","year":"2011","unstructured":"Kida, K. et al. Inhaled hydrogen sulfide prevents neurodegeneration and movement disorder in a mouse model of Parkinson\u2019s disease. Antioxid. Redox Signal. 15, 343\u2013352 (2011).","journal-title":"Antioxid. Redox Signal."},{"key":"BFsrep29808_CR55","doi-asserted-by":"crossref","unstructured":"Cuevasanta, E. et al. Reaction of hydrogen sulfide with disulfide and sulfenic acid to form the strongly nucleophilic persulfide. J. Biol. Chem. 10.1074\/jbc.M115.672816 (2015).","DOI":"10.1074\/jbc.M115.672816"},{"key":"BFsrep29808_CR56","doi-asserted-by":"publisher","first-page":"20002","DOI":"10.1074\/jbc.M113.466177","volume":"288","author":"PK Yadav","year":"2013","unstructured":"Yadav, P. K., Yamada, K., Chiku, T., Koutmos, M. & Banerjee, R. Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase. J. Biol. Chem. 288, 20002\u201320013 (2013).","journal-title":"J. Biol. Chem."},{"key":"BFsrep29808_CR57","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","volume":"6","author":"JR Wi\u015bniewski","year":"2009","unstructured":"Wi\u015bniewski, J. R., Zougman, A., Nagaraj, N. & Mann, M. Universal sample preparation method for proteome analysis. Nat. Methods 6, 359\u2013362 (2009).","journal-title":"Nat. Methods"},{"key":"BFsrep29808_CR58","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1038\/nprot.2007.261","volume":"2","author":"J Rappsilber","year":"2007","unstructured":"Rappsilber, J., Mann, M. & Ishihama, Y. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat. Protoc. 2, 1896\u20131906 (2007).","journal-title":"Nat. Protoc."},{"key":"BFsrep29808_CR59","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","volume":"4","author":"DW Huang","year":"2009","unstructured":"Huang, D. W., Lempicki, R. A. & Sherman, B. T. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 4, 44\u201357 (2009).","journal-title":"Nat. Protoc."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep29808","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep29808.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep29808.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T19:36:00Z","timestamp":1672860960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep29808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,14]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,9,30]]}},"alternative-id":["BFsrep29808"],"URL":"https:\/\/doi.org\/10.1038\/srep29808","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,14]]},"assertion":[{"value":"7 March 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29808"}}