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The aim of the study was to evaluate the association of\n                    <jats:italic>EDN1, NOS3, ACE<\/jats:italic>\n                    and\n                    <jats:italic>VEGFA<\/jats:italic>\n                    genes with PPHN. Neonates with respiratory distress were enrolled in the study, whose gestational age \u226534 weeks, age \u22643 days. They were divided into PPHN and non-PPHN group. The\n                    <jats:italic>EDN1, NOS3, ACE<\/jats:italic>\n                    and\n                    <jats:italic>VEGFA<\/jats:italic>\n                    genes were detected by next-generation sequencing and the results were validated by Sanger sequencing. Serum endothelin-1 (ET-1) levels were quantified by ELISA. A total of 112 neonates were enrolled (n\u2009=\u200955 in PPHN group; n\u2009=\u200957 in non-PPHN group). There is a significantly difference in the genotype distribution of\n                    <jats:italic>EDN1<\/jats:italic>\n                    rs2070699 between the PPHN and non-PPHN group (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090). A higher frequency of the rs2070699 T allele was observed in the PPHN group (54.5% vs 27.2%; OR\u2009=\u20093.89; 95%CI 1.96\u20137.72). The rs2070699 T allele was associated with higher ET-1 levels (3.333\u2009\u00b1\u20092.517 pg\/mL vs 1.223\u2009\u00b1\u20090.856 pg\/mL;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.002) and a longer ventilation period (5.8\u2009\u00b1\u20092.6 days vs 3.6\u2009\u00b1\u20093.3 days;\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090). The results suggest there is an association between\n                    <jats:italic>EDN1<\/jats:italic>\n                    and PPHN. The presence of the rs2070699 T allele increased the risk of PPHN in neonates with respiratory distress.\n                  <\/jats:p>","DOI":"10.1038\/srep29877","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T05:17:42Z","timestamp":1468819062000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["EDN1 Gene Variant is Associated with Neonatal Persistent Pulmonary Hypertension"],"prefix":"10.1038","volume":"6","author":[{"given":"Mei","family":"Mei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinqiao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,18]]},"reference":[{"key":"BFsrep29877_CR1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1542\/peds.105.1.14","volume":"105","author":"MC Walsh-Sukys","year":"2000","unstructured":"Walsh-Sukys, M. 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