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And this ternary composite was firstly used in construction of electrochemiluminescence (ECL) immunosensor due to its double-quenching effect of quantum dots (QDs). Based on the high electrocatalytic activity of ternary complexes for the reduction of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> which acted as the coreactant of QDs-based ECL, as a result, the ECL intensity of QDs decreased. Besides, due to the ECL resonance energy transfer (ECL-RET) strategy between the large amount of Au nanorods (Au NRs) on the ternary composite surface and the CdS:Eu QDs, the ECL intensity of QDs was further quenched. Based on the double-quenching effect, a novel ultrasensitive ECL immunoassay method for detection of carcinoembryonic antigen (CEA) which is used as a model biomarker analyte was proposed. The designed immunoassay method showed a linear range from 0.01\u2009pg mL<jats:sup>\u22121<\/jats:sup> to 1.0\u2009ng mL<jats:sup>\u22121<\/jats:sup> with a detection limit of 0.01\u2009pg mL<jats:sup>\u22121<\/jats:sup>. The method showing low detection limit, good stability and acceptable fabrication reproducibility, provided a new approach for ECL immunoassay sensing and significant prospect for practical application.<\/jats:p>","DOI":"10.1038\/srep30577","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T09:40:54Z","timestamp":1469612454000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay"],"prefix":"10.1038","volume":"6","author":[{"given":"Jing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meirong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shusheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,7,27]]},"reference":[{"key":"BFsrep30577_CR1","doi-asserted-by":"publisher","first-page":"8378","DOI":"10.1021\/ja0613106","volume":"128","author":"SI Stoeva","year":"2006","unstructured":"Stoeva, S. 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