{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:07:34Z","timestamp":1790510854467,"version":"4.1.0"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T00:00:00Z","timestamp":1470182400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T00:00:00Z","timestamp":1470182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tight regulation of kinesin activity is crucial and malfunction is linked to neurological diseases. Point mutations in the <jats:italic>KIF21A<\/jats:italic> gene cause congenital fibrosis of the extraocular muscles type 1 (CFEOM1) by disrupting the autoinhibitory interaction between the motor domain and a regulatory region in the stalk. However, the molecular mechanism underlying the misregulation of KIF21A activity in CFEOM1 is not understood. Here, we show that the KIF21A regulatory domain containing all disease-associated substitutions in the stalk forms an intramolecular antiparallel coiled coil that inhibits the kinesin. CFEOM1 mutations lead to KIF21A hyperactivation by affecting either the structural integrity of the antiparallel coiled coil or the autoinhibitory binding interface, thereby reducing its affinity for the motor domain. Interaction of the KIF21A regulatory domain with the KIF21B motor domain and sequence similarities to KIF7 and KIF27 strongly suggest a conservation of this regulatory mechanism in other kinesin-4 family members.<\/jats:p>","DOI":"10.1038\/srep30668","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T09:31:28Z","timestamp":1470216688000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations"],"prefix":"10.1038","volume":"6","author":[{"given":"Sarah","family":"Bianchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelmina E.","family":"van Riel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian H. W.","family":"Kraatz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natacha","family":"Olieric","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Frey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene A.","family":"Katrukha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Jaussi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Missimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya","family":"Grigoriev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Olieric","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger M.","family":"Benoit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel O.","family":"Steinmetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Akhmanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Kammerer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,8,3]]},"reference":[{"key":"BFsrep30668_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1083\/jcb.200408113","volume":"167","author":"CJ Lawrence","year":"2004","unstructured":"Lawrence, C. J. et al. A standardized kinesin nomenclature. J Cell Biol 167, 19\u201322, doi: 10.1083\/jcb.200408113 (2004).","journal-title":"J Cell Biol"},{"key":"BFsrep30668_CR2","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1111\/cas.12138","volume":"104","author":"X Liu","year":"2013","unstructured":"Liu, X., Gong, H. & Huang, K. Oncogenic role of kinesin proteins and targeting kinesin therapy. Cancer Sci 104, 651\u2013656, doi: 10.1111\/cas.12138 (2013).","journal-title":"Cancer Sci"},{"key":"BFsrep30668_CR3","doi-asserted-by":"publisher","first-page":"12776","DOI":"10.1523\/JNEUROSCI.3463-09.2009","volume":"29","author":"GA Morfini","year":"2009","unstructured":"Morfini, G. A. et al. Axonal transport defects in neurodegenerative diseases. J Neurosci 29, 12776\u201312786, doi: 10.1523\/JNEUROSCI.3463-09.2009 (2009).","journal-title":"J Neurosci"},{"key":"BFsrep30668_CR4","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/j.ceb.2010.04.008","volume":"22","author":"A Akhmanova","year":"2010","unstructured":"Akhmanova, A. & Hammer, J. A. 3rd . Linking molecular motors to membrane cargo. Curr Opin Cell Biol 22, 479\u2013487, doi: 10.1016\/j.ceb.2010.04.008 (2010).","journal-title":"Curr Opin Cell Biol"},{"key":"BFsrep30668_CR5","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1038\/nrm3227","volume":"12","author":"C Janke","year":"2011","unstructured":"Janke, C. & Bulinski, J. C. Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions. Nat Rev Mol Cell Biol 12, 773\u2013786, doi: 10.1038\/nrm3227 (2011).","journal-title":"Nat Rev Mol Cell Biol"},{"key":"BFsrep30668_CR6","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/1756-6606-2-25","volume":"2","author":"MA Schlager","year":"2009","unstructured":"Schlager, M. A. & Hoogenraad, C. C. Basic mechanisms for recognition and transport of synaptic cargos. Mol Brain 2, 25, doi: 10.1186\/1756-6606-2-25 (2009).","journal-title":"Mol Brain"},{"key":"BFsrep30668_CR7","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1038\/nrm2782","volume":"10","author":"KJ Verhey","year":"2009","unstructured":"Verhey, K. J. & Hammond, J. W. Traffic control: regulation of kinesin motors. Nat Rev Mol Cell Biol 10, 765\u2013777, doi: 10.1038\/nrm2782 (2009).","journal-title":"Nat Rev Mol Cell Biol"},{"key":"BFsrep30668_CR8","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1126\/science.1204824","volume":"333","author":"HY Kaan","year":"2011","unstructured":"Kaan, H. Y., Hackney, D. D. & Kozielski, F. The structure of the kinesin-1 motor-tail complex reveals the mechanism of autoinhibition. Science 333, 883\u2013885, doi: 10.1126\/science.1204824 (2011).","journal-title":"Science"},{"key":"BFsrep30668_CR9","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1083\/jcb.143.4.1053","volume":"143","author":"KJ Verhey","year":"1998","unstructured":"Verhey, K. J. et al. Light chain-dependent regulation of Kinesin\u2019s interaction with microtubules. J Cell Biol 143, 1053\u20131066 (1998).","journal-title":"J Cell Biol"},{"key":"BFsrep30668_CR10","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1083\/jcb.201001057","volume":"189","author":"JW Hammond","year":"2010","unstructured":"Hammond, J. W., Blasius, T. L., Soppina, V., Cai, D. & Verhey, K. J. Autoinhibition of the kinesin-2 motor KIF17 via dual intramolecular mechanisms. J Cell Biol 189, 1013\u20131025, doi: 10.1083\/jcb.201001057 (2010).","journal-title":"J Cell Biol"},{"key":"BFsrep30668_CR11","doi-asserted-by":"publisher","first-page":"1550","DOI":"10.1016\/j.str.2012.07.002","volume":"20","author":"L Huo","year":"2012","unstructured":"Huo, L. et al. The CC1-FHA tandem as a central hub for controlling the dimerization and activation of kinesin-3 KIF1A. Structure 20, 1550\u20131561, doi: 10.1016\/j.str.2012.07.002 (2012).","journal-title":"Structure"},{"key":"BFsrep30668_CR12","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.neuron.2014.02.038","volume":"82","author":"L Cheng","year":"2014","unstructured":"Cheng, L. et al. Human CFEOM1 mutations attenuate KIF21A autoinhibition and cause oculomotor axon stalling. Neuron 82, 334\u2013349, doi: 10.1016\/j.neuron.2014.02.038 (2014).","journal-title":"Neuron"},{"key":"BFsrep30668_CR13","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.devcel.2013.09.010","volume":"27","author":"B van der Vaart","year":"2013","unstructured":"van der Vaart, B. et al. CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor. Dev Cell 27, 145\u2013160, doi: 10.1016\/j.devcel.2013.09.010 (2013).","journal-title":"Dev Cell"},{"key":"BFsrep30668_CR14","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1083\/jcb.145.3.469","volume":"145","author":"JR Marszalek","year":"1999","unstructured":"Marszalek, J. R., Weiner, J. A., Farlow, S. J., Chun, J. & Goldstein, L. S. Novel dendritic kinesin sorting identified by different process targeting of two related kinesins: KIF21A and KIF21B. J Cell Biol 145, 469\u2013479 (1999).","journal-title":"J Cell Biol"},{"key":"BFsrep30668_CR15","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/ng1261","volume":"35","author":"K Yamada","year":"2003","unstructured":"Yamada, K. et al. Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1). Nat Genet 35, 318\u2013321, doi: 10.1038\/ng1261 (2003).","journal-title":"Nat Genet"},{"key":"BFsrep30668_CR16","unstructured":"Andrews, C. V., Hunter, D. G. & Engle, E. C. In GeneReviews(R) (eds R. A. Pagon et al.) (University of Washington, Seattle, 1993)."},{"key":"BFsrep30668_CR17","doi-asserted-by":"publisher","first-page":"973","DOI":"10.3892\/ijmm.2011.759","volume":"28","author":"P Wang","year":"2011","unstructured":"Wang, P., Li, S., Xiao, X., Guo, X. & Zhang, Q. KIF21A novel deletion and recurrent mutation in patients with congenital fibrosis of the extraocular muscles-1. Int J Mol Med 28, 973\u2013975, doi: 10.3892\/ijmm.2011.759 (2011).","journal-title":"Int J Mol Med"},{"key":"BFsrep30668_CR18","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1126\/science.2237415","volume":"250","author":"KT O\u2019Neil","year":"1990","unstructured":"O\u2019Neil, K. T. & DeGrado, W. F. A thermodynamic scale for the helix-forming tendencies of the commonly occurring amino acids. Science 250, 646\u2013651 (1990).","journal-title":"Science"},{"key":"BFsrep30668_CR19","doi-asserted-by":"publisher","first-page":"2544","DOI":"10.1021\/bi962391t","volume":"36","author":"MG Oakley","year":"1997","unstructured":"Oakley, M. G. & Kim, P. S. Protein dissection of the antiparallel coiled coil from Escherichia coli seryl tRNA synthetase. Biochemistry 36, 2544\u20132549, doi: 10.1021\/bi962391t (1997).","journal-title":"Biochemistry"},{"key":"BFsrep30668_CR20","doi-asserted-by":"publisher","first-page":"e74448","DOI":"10.1371\/journal.pone.0074448","volume":"8","author":"I Sen","year":"2013","unstructured":"Sen, I., Veprintsev, D., Akhmanova, A. & Steinmetz, M. O. End binding proteins are obligatory dimers. PLoS One 8, e74448, doi: 10.1371\/journal.pone.0074448 (2013).","journal-title":"PLoS One"},{"key":"BFsrep30668_CR21","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1242\/jcs.113.12.2285","volume":"113","author":"CC Hoogenraad","year":"2000","unstructured":"Hoogenraad, C. C., Akhmanova, A., Grosveld, F., De Zeeuw, C. I. & Galjart, N. Functional analysis of CLIP-115 and its binding to microtubules. J Cell Sci 113 (Pt 12), 2285\u20132297 (2000).","journal-title":"J Cell Sci"},{"key":"BFsrep30668_CR22","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1126\/science.2911757","volume":"243","author":"EK O\u2019Shea","year":"1989","unstructured":"O\u2019Shea, E. K., Rutkowski, R. & Kim, P. S. Evidence that the leucine zipper is a coiled coil. Science 243, 538\u2013542 (1989).","journal-title":"Science"},{"key":"BFsrep30668_CR23","doi-asserted-by":"publisher","first-page":"8938","DOI":"10.1073\/pnas.0803575105","volume":"105","author":"KA Dietrich","year":"2008","unstructured":"Dietrich, K. A. et al. The kinesin-1 motor protein is regulated by a direct interaction of its head and tail. Proc Natl Acad Sci USA 105, 8938\u20138943, doi: 10.1073\/pnas.0803575105 (2008).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep30668_CR24","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1001\/archopht.123.9.1254","volume":"123","author":"K Yamada","year":"2005","unstructured":"Yamada, K., Hunter, D. G., Andrews, C. & Engle, E. C. A novel KIF21A mutation in a patient with congenital fibrosis of the extraocular muscles and Marcus Gunn jaw-winking phenomenon. Arch Ophthalmol 123, 1254\u20131259, doi: 10.1001\/archopht.123.9.1254 (2005).","journal-title":"Arch Ophthalmol"},{"key":"BFsrep30668_CR25","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/1471-2156-8-26","volume":"8","author":"WM Chan","year":"2007","unstructured":"Chan, W. M. et al. Three novel mutations in KIF21A highlight the importance of the third coiled-coil stalk domain in the etiology of CFEOM1. BMC Genet 8, 26, doi: 10.1186\/1471-2156-8-26 (2007).","journal-title":"BMC Genet"},{"key":"BFsrep30668_CR26","first-page":"368","volume":"20","author":"Z Ali","year":"2014","unstructured":"Ali, Z. et al. A novel de novo KIF21A mutation in a patient with congenital fibrosis of the extraocular muscles and Mobius syndrome. Mol Vis 20, 368\u2013375 (2014).","journal-title":"Mol Vis"},{"key":"BFsrep30668_CR27","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1038\/380550a0","volume":"380","author":"FJ Kull","year":"1996","unstructured":"Kull, F. J., Sablin, E. P., Lau, R., Fletterick, R. J. & Vale, R. D. Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380, 550\u2013555, doi: 10.1038\/380550a0 (1996).","journal-title":"Nature"},{"key":"BFsrep30668_CR28","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1038\/380555a0","volume":"380","author":"EP Sablin","year":"1996","unstructured":"Sablin, E. P., Kull, F. J., Cooke, R., Vale, R. D. & Fletterick, R. J. Crystal structure of the motor domain of the kinesin-related motor ncd. Nature 380, 555\u2013559, doi: 10.1038\/380555a0 (1996).","journal-title":"Nature"},{"key":"BFsrep30668_CR29","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1021\/ja026939x","volume":"125","author":"C Dominguez","year":"2003","unstructured":"Dominguez, C., Boelens, R. & Bonvin, A. M. HADDOCK: a protein-protein docking approach based on biochemical or biophysical information. J Am Chem Soc 125, 1731\u20131737, doi: 10.1021\/ja026939x (2003).","journal-title":"J Am Chem Soc"},{"key":"BFsrep30668_CR30","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1038\/nsmb.2624","volume":"20","author":"B Gigant","year":"2013","unstructured":"Gigant, B. et al. Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol 20, 1001\u20131007, doi: 10.1038\/nsmb.2624 (2013).","journal-title":"Nat Struct Mol Biol"},{"key":"BFsrep30668_CR31","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1126\/science.1094838","volume":"303","author":"H Bringmann","year":"2004","unstructured":"Bringmann, H. et al. A kinesin-like motor inhibits microtubule dynamic instability. Science 303, 1519\u20131522, doi: 10.1126\/science.1094838 (2004).","journal-title":"Science"},{"key":"BFsrep30668_CR32","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1038\/ncb2988","volume":"16","author":"M He","year":"2014","unstructured":"He, M. et al. The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment. Nat Cell Biol 16, 663\u2013672, doi: 10.1038\/ncb2988 (2014).","journal-title":"Nat Cell Biol"},{"key":"BFsrep30668_CR33","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1038\/ng.826","volume":"43","author":"A Putoux","year":"2011","unstructured":"Putoux, A. et al. KIF7 mutations cause fetal hydrolethalus and acrocallosal syndromes. Nat Genet 43, 601\u2013606, doi: 10.1038\/ng.826 (2011).","journal-title":"Nat Genet"},{"key":"BFsrep30668_CR34","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1750-1172-7-27","volume":"7","author":"BR Ali","year":"2012","unstructured":"Ali, B. R., Silhavy, J. L., Akawi, N. A., Gleeson, J. G. & Al-Gazali, L. A mutation in KIF7 is responsible for the autosomal recessive syndrome of macrocephaly, multiple epiphyseal dysplasia and distinctive facial appearance. Orphanet J Rare Dis 7, 27, doi: 10.1186\/1750-1172-7-27 (2012).","journal-title":"Orphanet J Rare Dis"},{"key":"BFsrep30668_CR35","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.molcel.2008.01.004","volume":"29","author":"J Espeut","year":"2008","unstructured":"Espeut, J. et al. Phosphorylation relieves autoinhibition of the kinetochore motor Cenp-E. Mol Cell 29, 637\u2013643, doi: 10.1016\/j.molcel.2008.01.004 (2008).","journal-title":"Mol Cell"},{"key":"BFsrep30668_CR36","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1083\/jcb.200605099","volume":"176","author":"TL Blasius","year":"2007","unstructured":"Blasius, T. L., Cai, D., Jih, G. T., Toret, C. P. & Verhey, K. J. Two binding partners cooperate to activate the molecular motor Kinesin-1. J Cell Biol 176, 11\u201317, doi: 10.1083\/jcb.200605099 (2007).","journal-title":"J Cell Biol"},{"key":"BFsrep30668_CR37","doi-asserted-by":"publisher","first-page":"18788","DOI":"10.1073\/pnas.0810104105","volume":"105","author":"X Shen","year":"2008","unstructured":"Shen, X. et al. Interaction of brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and kinesin motor protein KIF21A. Proc Natl Acad Sci USA 105, 18788\u201318793, doi: 10.1073\/pnas.0810104105 (2008).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep30668_CR38","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/j.bbrc.2009.06.109","volume":"386","author":"N Kakinuma","year":"2009","unstructured":"Kakinuma, N. & Kiyama, R. A major mutation of KIF21A associated with congenital fibrosis of the extraocular muscles type 1 (CFEOM1) enhances translocation of Kank1 to the membrane. Biochem Biophys Res Commun 386, 639\u2013644, doi: 10.1016\/j.bbrc.2009.06.109 (2009).","journal-title":"Biochem Biophys Res Commun"},{"key":"BFsrep30668_CR39","doi-asserted-by":"publisher","first-page":"4102","DOI":"10.1523\/jneurosci.6331-11.2012","volume":"32","author":"KH Lee","year":"2012","unstructured":"Lee, K. H. et al. KIF21A-mediated axonal transport and selective endocytosis underlie the polarized targeting of NCKX2. J Neurosci 32, 4102\u20134117, doi: 10.1523\/jneurosci.6331-11.2012 (2012).","journal-title":"J Neurosci"},{"key":"BFsrep30668_CR40","doi-asserted-by":"publisher","first-page":"e75603","DOI":"10.1371\/journal.pone.0075603","volume":"8","author":"D Labonte","year":"2013","unstructured":"Labonte, D. et al. TRIM3 regulates the motility of the kinesin motor protein KIF21B. PLoS One 8, e75603, doi: 10.1371\/journal.pone.0075603 (2013).","journal-title":"PLoS One"},{"key":"BFsrep30668_CR41","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1186\/s40478-014-0144-4","volume":"2","author":"KL Kreft","year":"2014","unstructured":"Kreft, K. L. et al. Abundant kif21b is associated with accelerated progression in neurodegenerative diseases. Acta Neuropathol Commun 2, 144, doi: 10.1186\/s40478-014-0144-4 (2014).","journal-title":"Acta Neuropathol Commun"},{"key":"BFsrep30668_CR42","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/1472-6750-7-31","volume":"7","author":"D Frey","year":"2007","unstructured":"Frey, D., Kambach, C., Steinmetz, M. O. & Jaussi, R. Production of in vitro amplified DNA pseudolibraries and high-throughput cDNA target amplification. BMC Biotechnol 7, 31, doi: 10.1186\/1472-6750-7-31 (2007).","journal-title":"BMC Biotechnol"},{"key":"BFsrep30668_CR43","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1186\/1472-6750-10-56","volume":"10","author":"N Olieric","year":"2010","unstructured":"Olieric, N. et al. Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection. BMC Biotechnol 10, 56, doi: 10.1186\/1472-6750-10-56 (2010).","journal-title":"BMC Biotechnol"},{"key":"BFsrep30668_CR44","doi-asserted-by":"publisher","first-page":"10602","DOI":"10.1074\/jbc.273.17.10602","volume":"273","author":"RA Kammerer","year":"1998","unstructured":"Kammerer, R. A. et al. Tenascin-C hexabrachion assembly is a sequential two-step process initiated by coiled-coil alpha-helices. J Biol Chem 273, 10602\u201310608 (1998).","journal-title":"J Biol Chem"},{"key":"BFsrep30668_CR45","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1107\/S0907444909047337","volume":"66","author":"WXds Kabsch","year":"2010","unstructured":"Kabsch, W. Xds. Acta Crystallogr D Biol Crystallogr 66, 125\u2013132, doi: 10.1107\/S0907444909047337 (2010).","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFsrep30668_CR46","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1002\/prot.22540","volume":"77","author":"S Raman","year":"2009","unstructured":"Raman, S. et al. Structure prediction for CASP8 with all-atom refinement using Rosetta. Proteins 77 Suppl 9, 89\u201399, doi: 10.1002\/prot.22540 (2009).","journal-title":"Proteins"},{"key":"BFsrep30668_CR47","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.1107\/S0907444912039194","volume":"68","author":"J Bibby","year":"2012","unstructured":"Bibby, J., Keegan, R. M., Mayans, O., Winn, M. D. & Rigden, D. J. AMPLE: a cluster-and-truncate approach to solve the crystal structures of small proteins using rapidly computed ab initio models. Acta Crystallogr D Biol Crystallogr 68, 1622\u20131631, doi: 10.1107\/S0907444912039194 (2012).","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFsrep30668_CR48","doi-asserted-by":"publisher","first-page":"W526","DOI":"10.1093\/nar\/gkh468","volume":"32","author":"DE Kim","year":"2004","unstructured":"Kim, D. E., Chivian, D. & Baker, D. Protein structure prediction and analysis using the Robetta server. Nucleic Acids Res 32, W526\u2013W531, doi: 10.1093\/nar\/gkh468 (2004).","journal-title":"Nucleic Acids Res"},{"key":"BFsrep30668_CR49","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy, A. J. et al. Phaser crystallographic software. J Appl Crystallogr 40, 658\u2013674, doi: 10.1107\/S0021889807021206 (2007).","journal-title":"J Appl Crystallogr"},{"key":"BFsrep30668_CR50","first-page":"43","volume":"7","author":"PH Zwart","year":"2005","unstructured":"Zwart, P. H. G.-K., R.W. ; Adams, P.D. Xtriage and Fest: automatic assessment of X-ray data and substructure structure factor estimation. CCP4 Newsletter Contribution 7, 43 (2005).","journal-title":"CCP4 Newsletter"},{"key":"BFsrep30668_CR51","doi-asserted-by":"publisher","first-page":"1002","DOI":"10.1107\/S0907444906022116","volume":"62","author":"K Cowtan","year":"2006","unstructured":"Cowtan, K. The Buccaneer software for automated model building. 1. Tracing protein chains. Acta Crystallogr D Biol Crystallogr 62, 1002\u20131011, doi: 10.1107\/S0907444906022116 (2006).","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFsrep30668_CR52","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr 66, 213\u2013221, doi: 10.1107\/S0907444909052925 (2010).","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFsrep30668_CR53","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66, 486\u2013501, doi: 10.1107\/S0907444910007493 (2010).","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"BFsrep30668_CR54","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/S0907444909042073","volume":"66","author":"VB Chen","year":"2010","unstructured":"Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallographica Section D 66, 12\u201321, doi: 10.1107\/S0907444909042073 (2010).","journal-title":"Acta Crystallographica Section D"},{"key":"BFsrep30668_CR55","doi-asserted-by":"publisher","first-page":"2980","DOI":"10.1096\/fj.11-185397","volume":"25","author":"K Kisko","year":"2011","unstructured":"Kisko, K. et al. Structural analysis of vascular endothelial growth factor receptor-2\/ligand complexes by small-angle X-ray solution scattering. FASEB J 25, 2980\u20132986, doi: 10.1096\/fj.11-185397 (2011).","journal-title":"FASEB J"},{"key":"BFsrep30668_CR56","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1107\/s0021889812007662","volume":"45","author":"MV Petoukhov","year":"2012","unstructured":"Petoukhov, M. V. et al. New developments in the program package for small-angle scattering data analysis. J Appl Crystallogr 45, 342\u2013350, doi: 10.1107\/s0021889812007662 (2012).","journal-title":"J Appl Crystallogr"},{"key":"BFsrep30668_CR57","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1107\/S0021889803012779","volume":"36","author":"PV Konarev","year":"2003","unstructured":"Konarev, P. V., Volkov, V. V., Sokolova, A. V., Koch, M. H. J. & Svergun, D. I. PRIMUS: a Windows PC-based system for small-angle scattering data analysis. Journal of Applied Crystallography 36, 1277\u20131282, doi: 10.1107\/S0021889803012779 (2003).","journal-title":"Journal of Applied Crystallography"},{"key":"BFsrep30668_CR58","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1107\/S0021889892001663","volume":"25","author":"D Svergun","year":"1992","unstructured":"Svergun, D. Determination of the regularization parameter in indirect-transform methods using perceptual criteria. Journal of Applied Crystallography 25, 495\u2013503, doi: 10.1107\/S0021889892001663 (1992).","journal-title":"Journal of Applied Crystallography"},{"key":"BFsrep30668_CR59","doi-asserted-by":"publisher","first-page":"2879","DOI":"10.1016\/S0006-3495(99)77443-6","volume":"76","author":"DI Svergun","year":"1999","unstructured":"Svergun, D. I. Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys J 76, 2879\u20132886 (1999).","journal-title":"Biophys J"},{"key":"BFsrep30668_CR60","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1107\/S0021889809000338","volume":"42","author":"D Franke","year":"2009","unstructured":"Franke, D. & Svergun, D. I. DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering. Journal of Applied Crystallography 42, 342\u2013346, doi: 10.1107\/S0021889809000338 (2009).","journal-title":"Journal of Applied Crystallography"},{"key":"BFsrep30668_CR61","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1107\/S0021889803000268","volume":"36","author":"VV Volkov","year":"2003","unstructured":"Volkov, V. V. & Svergun, D. I. Uniqueness of ab initio shape determination in small-angle scattering. Journal of Applied Crystallography 36, 860\u2013864, doi: 10.1107\/S0021889803000268 (2003).","journal-title":"Journal of Applied Crystallography"},{"key":"BFsrep30668_CR62","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1107\/S0021889895007047","volume":"28","author":"D Svergun","year":"1995","unstructured":"Svergun, D., Barberato, C. & Koch, M. H. J. CRYSOL-a Program to Evaluate X-ray Solution Scattering of Biological Macromolecules from Atomic Coordinates. Journal of Applied Crystallography 28, 768\u2013773, doi: 10.1107\/S0021889895007047 (1995).","journal-title":"Journal of Applied Crystallography"},{"key":"BFsrep30668_CR63","doi-asserted-by":"publisher","first-page":"5066","DOI":"10.1021\/ac3007522","volume":"84","author":"S Keller","year":"2012","unstructured":"Keller, S. et al. High-precision isothermal titration calorimetry with automated peak-shape analysis. Anal Chem 84, 5066\u20135073, doi: 10.1021\/ac3007522 (2012).","journal-title":"Anal Chem"},{"key":"BFsrep30668_CR64","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1110\/ps.062558507","volume":"16","author":"JC Houtman","year":"2007","unstructured":"Houtman, J. C. et al. Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: application to adaptor protein complexes in cell signaling. Protein Sci 16, 30\u201342, doi: 10.1110\/ps.062558507 (2007).","journal-title":"Protein Sci"},{"key":"BFsrep30668_CR65","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1016\/j.neuron.2014.04.019","volume":"82","author":"KW Yau","year":"2014","unstructured":"Yau, K. W. et al. Microtubule minus-end binding protein CAMSAP2 controls axon specification and dendrite development. Neuron 82, 1058\u20131073, doi: 10.1016\/j.neuron.2014.04.019 (2014).","journal-title":"Neuron"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep30668","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep30668.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep30668.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T21:49:04Z","timestamp":1672868944000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep30668"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,3]]},"references-count":65,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,11,14]]}},"alternative-id":["BFsrep30668"],"URL":"https:\/\/doi.org\/10.1038\/srep30668","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,3]]},"assertion":[{"value":"17 November 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 July 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 August 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"30668"}}