{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:50:56Z","timestamp":1790394656442,"version":"4.1.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T00:00:00Z","timestamp":1469750400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T00:00:00Z","timestamp":1469750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parkinson\u2019s disease (PD) is a progressive, age-related, neurodegenerative disorder and oxidative stress is an important mediator in its pathogenesis. DJ-1, the product of the causative gene of a familial form of PD, plays a significant role in anti-oxidative defence to protect cells from oxidative stress. DJ-1 undergoes preferential oxidation at the cysteine residue at position 106 (Cys-106) under oxidative stress. Here, using specific antibodies against Cys-106-oxidized DJ-1 (oxDJ-1), it was found that the levels of oxDJ-1 in the erythrocytes of unmedicated PD patients (n\u2009=\u200988) were higher than in those of medicated PD patients (n\u2009=\u200962) and healthy control subjects (n\u2009=\u200933). Elevated oxDJ-1 levels were also observed in a non-human primate PD model. Biochemical analysis of oxDJ-1 in erythrocyte lysates showed that oxDJ-1 formed dimer and polymer forms and that the latter interacts with 20S proteasome. These results clearly indicate a biochemical alteration in the blood of PD patients, which could be utilized as an early diagnosis marker for PD.<\/jats:p>","DOI":"10.1038\/srep30793","type":"journal-article","created":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T09:29:55Z","timestamp":1469784595000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Oxidation and interaction of DJ-1 with 20S proteasome in the erythrocytes of early stage Parkinson\u2019s disease patients"],"prefix":"10.1038","volume":"6","author":[{"given":"Yoshiro","family":"Saito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Akazawa-Ogawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Matsumura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumasa","family":"Saigoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayoko","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Sutou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuka","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Mita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mototada","family":"Shichiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Hisahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Hara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harutoshi","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Takamatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Hagihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasukazu","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Hamakubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Kusunoki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Shimohama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,29]]},"reference":[{"key":"BFsrep30793_CR1","doi-asserted-by":"publisher","first-page":"683920","DOI":"10.1155\/2013\/683920","volume":"2013","author":"H Ariga","year":"2013","unstructured":"Ariga, H. et al. Neuroprotective function of DJ-1 in Parkinson\u2019s disease. Oxid. Med. Cell. Longevity 2013, 683920 (2013).","journal-title":"Oxid. Med. Cell. Longevity"},{"key":"BFsrep30793_CR2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/sj.embor.7400074","volume":"5","author":"T Taira","year":"2004","unstructured":"Taira, T. et al. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep. 5, 213\u2013218 (2004).","journal-title":"EMBO Rep."},{"key":"BFsrep30793_CR3","doi-asserted-by":"publisher","first-page":"S44","DOI":"10.1002\/mds.22713","volume":"25","author":"MR Cookson","year":"2010","unstructured":"Cookson, M. R. DJ-1, PINK1 and their effects on mitochondrial pathways. Movement Disord. 25 Suppl 1, S44\u2013S48 (2010).","journal-title":"Movement Disord."},{"key":"BFsrep30793_CR4","doi-asserted-by":"publisher","first-page":"138","DOI":"10.3164\/jcbn.13-108","volume":"54","author":"Y Saito","year":"2014","unstructured":"Saito, Y. Oxidized DJ-1 as a possible biomarker of Parkinson\u2019s disease. J. Clin. Biochem. Nutr. 54, 138\u2013144 (2014).","journal-title":"J. Clin. Biochem. Nutr."},{"key":"BFsrep30793_CR5","doi-asserted-by":"publisher","first-page":"3013","DOI":"10.1093\/hmg\/dds131","volume":"21","author":"JY Im","year":"2012","unstructured":"Im, J. Y., Lee, K. W., Woo, J. M., Junn, E. & Mouradian, M. M. DJ-1 induces thioredoxin 1 expression through the Nrf2 pathway. Hum. Mol. Genet. 21, 3013\u20133024 (2012).","journal-title":"Hum. Mol. Genet."},{"key":"BFsrep30793_CR6","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1128\/MCB.01350-12","volume":"33","author":"I Kato","year":"2013","unstructured":"Kato, I. et al. Oxidized DJ-1 inhibits p53 by sequestering p53 from promoters in a DNA-binding affinity-dependent manner. Mol. Cell. Biol. 33, 340\u2013359 (2013).","journal-title":"Mol. Cell. Biol."},{"key":"BFsrep30793_CR7","doi-asserted-by":"publisher","first-page":"43150","DOI":"10.1074\/jbc.M507124200","volume":"280","author":"W Zhou","year":"2005","unstructured":"Zhou, W. & Freed, C. R. DJ-1 up-regulates glutathione synthesis during oxidative stress and inhibits A53T alpha-synuclein toxicity. J. Biol. Chem. 280, 43150\u201343158 (2005).","journal-title":"J. Biol. Chem."},{"key":"BFsrep30793_CR8","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1038\/nature09536","volume":"468","author":"JN Guzman","year":"2010","unstructured":"Guzman, J. N. et al. Oxidant stress evoked by pacemaking in dopaminergic neurons is attenuated by DJ-1. Nature 468, 696\u2013700 (2010).","journal-title":"Nature"},{"key":"BFsrep30793_CR9","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.ccr.2005.02.010","volume":"7","author":"RH Kim","year":"2005","unstructured":"Kim, R. H. et al. DJ-1, a novel regulator of the tumor suppressor PTEN. Cancer Cell 7, 263\u2013273 (2005).","journal-title":"Cancer Cell"},{"key":"BFsrep30793_CR10","doi-asserted-by":"publisher","first-page":"14245","DOI":"10.1074\/jbc.M806902200","volume":"284","author":"J Waak","year":"2009","unstructured":"Waak, J. et al. Oxidizable residues mediating protein stability and cytoprotective interaction of DJ-1 with apoptosis signal-regulating kinase 1. J. Biol. Chem. 284, 14245\u201314257 (2009).","journal-title":"J. Biol. Chem."},{"key":"BFsrep30793_CR11","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1093\/cvr\/cvs302","volume":"97","author":"J Klawitter","year":"2013","unstructured":"Klawitter, J. et al. Association of DJ-1\/PTEN\/AKT- and ASK1\/p38-mediated cell signalling with ischaemic cardiomyopathy. Cardiovasc. Res. 97, 66\u201376 (2013).","journal-title":"Cardiovasc. Res."},{"key":"BFsrep30793_CR12","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1093\/hmg\/ddt466","volume":"23","author":"MU Sajjad","year":"2014","unstructured":"Sajjad, M. U. et al. DJ-1 modulates aggregation and pathogenesis in models of Huntington\u2019s disease. Hum. Mol. Genet. 23, 755\u2013766 (2014).","journal-title":"Hum. Mol. Genet."},{"key":"BFsrep30793_CR13","doi-asserted-by":"publisher","first-page":"6609","DOI":"10.1038\/ncomms7609","volume":"6","author":"O Moscovitz","year":"2015","unstructured":"Moscovitz, O. et al. The Parkinson\u2019s-associated protein DJ-1 regulates the 20S proteasome. Nat. Commun. 6, 6609, doi: 10.1038\/ncomms7609 (2015).","journal-title":"Nat. Commun."},{"key":"BFsrep30793_CR14","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1016\/j.bbrc.2004.03.110","volume":"317","author":"T Kinumi","year":"2004","unstructured":"Kinumi, T., Kimata, J., Taira, T., Ariga, H. & Niki, E. Cysteine-106 of DJ-1 is the most sensitive cysteine residue to hydrogen peroxide-mediated oxidation in vivo in human umbilical vein endothelial cells. Biochem. Biophys. Res. Comm. 317, 722\u2013728 (2004).","journal-title":"Biochem. Biophys. Res. Comm"},{"key":"BFsrep30793_CR15","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1089\/ars.2010.3481","volume":"15","author":"MA Wilson","year":"2011","unstructured":"Wilson, M. A. The role of cysteine oxidation in DJ-1 function and dysfunction. Antioxid. Redox Signal 15, 111\u2013122 (2011).","journal-title":"Antioxid. Redox Signal"},{"key":"BFsrep30793_CR16","doi-asserted-by":"publisher","first-page":"7430","DOI":"10.1021\/bi800282d","volume":"47","author":"AC Witt","year":"2008","unstructured":"Witt, A. C. et al. Cysteine pKa depression by a protonated glutamic acid in human DJ-1. Biochemistry 47, 7430\u20137440 (2008).","journal-title":"Biochemistry"},{"key":"BFsrep30793_CR17","doi-asserted-by":"publisher","first-page":"6476","DOI":"10.1074\/jbc.M806599200","volume":"284","author":"J Blackinton","year":"2009","unstructured":"Blackinton, J. et al. Formation of a stabilized cysteine sulfinic acid is critical for the mitochondrial function of the parkinsonism protein DJ-1. J. Biol. Chem. 284, 6476\u20136485 (2009).","journal-title":"J. Biol. Chem."},{"key":"BFsrep30793_CR18","doi-asserted-by":"publisher","first-page":"1354","DOI":"10.1016\/j.freeradbiomed.2009.08.003","volume":"47","author":"PJ Kahle","year":"2009","unstructured":"Kahle, P. J., Waak, J. & Gasser, T. DJ-1 and prevention of oxidative stress in Parkinson\u2019s disease and other age-related disorders. Free Rad. Biol. Med. 47, 1354\u20131361 (2009).","journal-title":"Free Rad. Biol. Med"},{"key":"BFsrep30793_CR19","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1136\/jnnp.55.3.181","volume":"55","author":"AJ Hughes","year":"1992","unstructured":"Hughes, A. J., Daniel, S. E., Kilford, L. & Lees, A. J. Accuracy of clinical diagnosis of idiopathic Parkinson\u2019s disease: a clinico-pathological study of 100 cases. J. Neurol. Neurosur. Psych. 55, 181\u2013184 (1992).","journal-title":"J. Neurol. Neurosur. Psych"},{"key":"BFsrep30793_CR20","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1097\/00005072-199603000-00001","volume":"55","author":"LS Forno","year":"1996","unstructured":"Forno, L. S. Neuropathology of Parkinson\u2019s disease. J. Neuropath. Exp. Neurol. 55, 259\u2013272 (1996).","journal-title":"J. Neuropath. Exp. Neurol."},{"key":"BFsrep30793_CR21","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s00441-004-0956-9","volume":"318","author":"H Braak","year":"2004","unstructured":"Braak, H., Ghebremedhin, E., Rub, U., Bratzke, H. & Del Tredici, K. Stages in the development of Parkinson\u2019s disease-related pathology. Cell Tissue Res. 318, 121\u2013134 (2004).","journal-title":"Cell Tissue Res."},{"key":"BFsrep30793_CR22","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1021\/tx700210j","volume":"21","author":"LM Sayre","year":"2008","unstructured":"Sayre, L. M., Perry, G. & Smith, M. A. Oxidative stress and neurotoxicity. Chem. Res. Toxicol. 21, 172\u2013188 (2008).","journal-title":"Chem. Res. Toxicol."},{"key":"BFsrep30793_CR23","doi-asserted-by":"crossref","unstructured":"Dias, V., Junn, E. & Mouradian, M. M. The Role of Oxidative Stress in Parkinson\u2019s Disease. J. Parkinson\u2019s Dis. 130230 (2013).","DOI":"10.3233\/JPD-130230"},{"key":"BFsrep30793_CR24","doi-asserted-by":"publisher","first-page":"S94","DOI":"10.1002\/ana.410320716","volume":"32","author":"DT Dexter","year":"1992","unstructured":"Dexter, D. T., Jenner, P., Schapira, A. H. & Marsden, C. D. Alterations in levels of iron, ferritin and other trace metals in neurodegenerative diseases affecting the basal ganglia. The Royal Kings and Queens Parkinson\u2019s Disease Research Group. Ann. Neurol. 32 Suppl, S94\u2013100 (1992).","journal-title":"Ann. Neurol."},{"key":"BFsrep30793_CR25","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/0304-3940(82)90390-1","volume":"33","author":"TL Perry","year":"1982","unstructured":"Perry, T. L., Godin, D. V. & Hansen, S. Parkinson\u2019s disease: a disorder due to nigral glutathione deficiency? Neurosci. Lett. 33, 305\u2013310 (1982).","journal-title":"Neurosci. Lett."},{"key":"BFsrep30793_CR26","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/1750-1326-6-8","volume":"6","author":"FP Bellinger","year":"2011","unstructured":"Bellinger, F. P. et al. Glutathione Peroxidase 4 is associated with Neuromelanin in Substantia Nigra and Dystrophic Axons in Putamen of Parkinson\u2019s brain. Mol. Neurodegener. 6, 8, doi: 10.1186\/1750-1326-6-8 (2011).","journal-title":"Mol. Neurodegener"},{"key":"BFsrep30793_CR27","doi-asserted-by":"publisher","first-page":"S11","DOI":"10.1038\/466S11a","volume":"466","author":"R Jones","year":"2010","unstructured":"Jones, R. Biomarkers: casting the net wide. Nature 466, S11\u2013S12 (2010).","journal-title":"Nature"},{"key":"BFsrep30793_CR28","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1007\/BF01728311","volume":"24","author":"J Booij","year":"1997","unstructured":"Booij, J. et al. Practical benefit of [123I]FP-CIT SPET in the demonstration of the dopaminergic deficit in Parkinson\u2019s disease. Eur. J. Nucl. Med. 24, 68\u201371 (1997).","journal-title":"Eur. J. Nucl. Med."},{"key":"BFsrep30793_CR29","doi-asserted-by":"publisher","first-page":"1820","DOI":"10.1212\/01.wnl.0000262033.01945.9a","volume":"68","author":"AE Oakley","year":"2007","unstructured":"Oakley, A. E. et al. Individual dopaminergic neurons show raised iron levels in Parkinson disease. Neurology 68, 1820\u20131825 (2007).","journal-title":"Neurology"},{"key":"BFsrep30793_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.neulet.2009.08.074","volume":"465","author":"Y Saito","year":"2009","unstructured":"Saito, Y. et al. Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. Neurosci. Lett. 465, 1\u20135 (2009).","journal-title":"Neurosci. Lett."},{"key":"BFsrep30793_CR31","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1097\/NEN.0000000000000087","volume":"73","author":"Y Saito","year":"2014","unstructured":"Saito, Y. et al. Immunostaining of oxidized DJ-1 in human and mouse brains. J. Neuropath. Exp. Neurol. 73, 714\u2013728 (2014).","journal-title":"J. Neuropath. Exp. Neurol."},{"key":"BFsrep30793_CR32","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.bcmd.2010.07.014","volume":"45","author":"X Xu","year":"2010","unstructured":"Xu, X., Martin, F. & Friedman, J. S. The familial Parkinson\u2019s disease gene DJ-1 (PARK7) is expressed in red cells and plays a role in protection against oxidative damage. Blood Cells Mol. Dis. 45, 227\u2013232 (2010).","journal-title":"Blood Cells Mol. Dis."},{"key":"BFsrep30793_CR33","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1038\/srep00954","volume":"2","author":"X Lin","year":"2012","unstructured":"Lin, X. et al. DJ-1 isoforms in whole blood as potential biomarkers of Parkinson disease. Sci. Rep. 2, 954, doi: 10.1038\/srep00954 (2012).","journal-title":"Sci. Rep."},{"key":"BFsrep30793_CR34","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.neulet.2015.05.030","volume":"599","author":"X Wang","year":"2015","unstructured":"Wang, X., Yu, S., Li, F. & Feng, T. Detection of alpha-synuclein oligomers in red blood cells as a potential biomarker of Parkinson\u2019s disease. Neurosci. Lett. 599, 115\u2013119 (2015).","journal-title":"Neurosci. Lett."},{"key":"BFsrep30793_CR35","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.jns.2011.11.001","volume":"314","author":"DO Cristalli","year":"2012","unstructured":"Cristalli, D. O., Arnal, N., Marra, F. A., de Alaniz, M. J. & Marra, C. A. Peripheral markers in neurodegenerative patients and their first-degree relatives. J. Neurol. Sci. 314, 48\u201356 (2012).","journal-title":"J. Neurol. Sci."},{"key":"BFsrep30793_CR36","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/j.nbd.2008.11.011","volume":"33","author":"CM Chen","year":"2009","unstructured":"Chen, C. M. et al. Increased oxidative damage in peripheral blood correlates with severity of Parkinson\u2019s disease. Neurobiol. Dis. 33, 429\u2013435 (2009).","journal-title":"Neurobiol. Dis."},{"key":"BFsrep30793_CR37","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.neulet.2010.08.007","volume":"483","author":"YO Akazawa","year":"2010","unstructured":"Akazawa, Y. O. et al. Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals. Neurosci. Lett. 483, 201\u2013205 (2010).","journal-title":"Neurosci. Lett."},{"key":"BFsrep30793_CR38","doi-asserted-by":"publisher","first-page":"S125","DOI":"10.1002\/ana.410320721","volume":"32","author":"DB Calne","year":"1992","unstructured":"Calne, D. B., Snow, B. J. & Lee, C. Criteria for diagnosing Parkinson\u2019s disease. Ann. Neurol. 32 Suppl, S125\u2013S127 (1992).","journal-title":"Ann. Neurol."},{"key":"BFsrep30793_CR39","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1212\/WNL.17.5.427","volume":"17","author":"MM Hoehn","year":"1967","unstructured":"Hoehn, M. M. & Yahr, M. D. Parkinsonism: onset, progression and mortality. Neurology 17, 427\u2013442 (1967).","journal-title":"Neurology"},{"key":"BFsrep30793_CR40","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/S0006-8993(03)03382-1","volume":"990","author":"JS Schneider","year":"2003","unstructured":"Schneider, J. S., Gonczi, H. & Decamp, E. Development of levodopa-induced dyskinesias in parkinsonian monkeys may depend upon rate of symptom onset and\/or duration of symptoms. Brain Res. 990, 38\u201344 (2003).","journal-title":"Brain Res."},{"key":"BFsrep30793_CR41","doi-asserted-by":"publisher","first-page":"4388","DOI":"10.1021\/pr200225c","volume":"10","author":"CB Knobbe","year":"2011","unstructured":"Knobbe, C. B. et al. Choice of biological source material supersedes oxidative stress in its influence on DJ-1 in vivo interactions with Hsp90. J. Proteome Res. 10, 4388\u20134404 (2011).","journal-title":"J. Proteome Res."},{"key":"BFsrep30793_CR42","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1080\/10715760100300831","volume":"35","author":"A Mitsumoto","year":"2001","unstructured":"Mitsumoto, A. et al. Oxidized forms of peroxiredoxins and DJ-1 on two-dimensional gels increased in response to sublethal levels of paraquat. Free Radic. Res. 35, 301\u2013310 (2001).","journal-title":"Free Radic. Res."},{"key":"BFsrep30793_CR43","doi-asserted-by":"publisher","first-page":"e27883","DOI":"10.1371\/journal.pone.0027883","volume":"6","author":"A Miyama","year":"2011","unstructured":"Miyama, A. et al. Oxidation of DJ-1 induced by 6-hydroxydopamine decreasing intracellular glutathione. PloS One 6, e27883 (2011).","journal-title":"PloS One"},{"key":"BFsrep30793_CR44","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1007\/s00401-008-0345-7","volume":"115","author":"DW Dickson","year":"2008","unstructured":"Dickson, D. W. et al. Evidence that incidental Lewy body disease is pre-symptomatic Parkinson\u2019s disease. Acta Neuropathol. 115, 437\u2013444 (2008).","journal-title":"Acta Neuropathol."},{"key":"BFsrep30793_CR45","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1111\/j.1750-3639.2006.00032.x","volume":"17","author":"S Orimo","year":"2007","unstructured":"Orimo, S. et al. Degeneration of cardiac sympathetic nerve begins in the early disease process of Parkinson\u2019s disease. Brain Pathol. 17, 24\u201330 (2007).","journal-title":"Brain Pathol."},{"key":"BFsrep30793_CR46","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1016\/j.parkreldis.2012.01.009","volume":"18","author":"S Orimo","year":"2012","unstructured":"Orimo, S., Suzuki, M., Inaba, A. & Mizusawa, H. 123I-MIBG myocardial scintigraphy for differentiating Parkinson\u2019s disease from other neurodegenerative parkinsonism: a systematic review and meta-analysis. Parkinsonism Relat. Disord. 18, 494\u2013500 (2012).","journal-title":"Parkinsonism Relat. Disord."},{"key":"BFsrep30793_CR47","first-page":"471","volume":"66","author":"I Ogawa","year":"2014","unstructured":"Ogawa, I. et al. The significance of oxidized DJ-1 protein (oxDJ-1) as a biomarker for Parkinson\u2019s disease. Brain Nerve 66, 471\u2013477 (2014).","journal-title":"Brain Nerve"},{"key":"BFsrep30793_CR48","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1093\/jnen\/63.7.742","volume":"63","author":"Y Saito","year":"2004","unstructured":"Saito, Y. et al. Lewy body-related alpha-synucleinopathy in aging. J. Neuropathol. Exp. Neurol. 63, 742\u2013749 (2004).","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"BFsrep30793_CR49","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.1177\/22.12.1084","volume":"22","author":"PK Nakane","year":"1974","unstructured":"Nakane, P. K. & Kawaoi, A. Peroxidase-labeled antibody. A new method of conjugation. J. Histochem. Cytochem. 22, 1084\u20131091 (1974).","journal-title":"J. Histochem. Cytochem."},{"key":"BFsrep30793_CR50","first-page":"3328","volume":"7","author":"F Le Naour","year":"2001","unstructured":"Le Naour, F. et al. Proteomics-based identification of RS\/DJ-1 as a novel circulating tumor antigen in breast cancer. Clin. Cancer Res. 7, 3328\u20133335 (2001).","journal-title":"Clin. Cancer Res."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep30793","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep30793.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep30793.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T21:36:38Z","timestamp":1672868198000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep30793"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,29]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,11,14]]}},"alternative-id":["BFsrep30793"],"URL":"https:\/\/doi.org\/10.1038\/srep30793","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,29]]},"assertion":[{"value":"29 February 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 July 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"30793"}}