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The Au-Fe nanoagglomerates were directly injected into PM droplets (PM dissolved in dichloromethane) in a collison atomizer and subsequently heat-treated to liberate the solvent from the droplets, resulting in the formation of PM-capped Au-Fe nanoagglomerates. Measured <jats:italic>in vitro<\/jats:italic>, the cytotoxicities of the nanoagglomerates (&gt;98.5% cell viability) showed no significant differences compared with PM particles alone (&gt;98.8%), thus implying that the nanoagglomerates are suitable for further testing of biofunctionalities. Measurements of gene delivery performance revealed that the incorporation of the Au-Fe nanoagglomerates enhanced the gene delivery performance (3.2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> RLU mg<jats:sup>\u22121<\/jats:sup>) of the PM particles alone (2.1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> RLU mg<jats:sup>\u22121<\/jats:sup>), which may have been caused by the PM structural change from a spherical to a hairy structure (i.e., the change followed the agglomerated backbone). Combining the X-ray-absorbing ability of Au and the magnetic property of Fe led to magnetic resonance (MR)-computed tomography (CT) contrast ability in a phantom; and the signal intensities [which reached 64 s<jats:sup>\u22121<\/jats:sup><jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>-relaxation in MR and 194 Hounsfield units (HUs) in CT at 6.0\u2009mg mL<jats:sup>\u22121<\/jats:sup>] depended on particle concentration (0.5\u20136.0\u2009mg mL<jats:sup>\u22121<\/jats:sup>).<\/jats:p>","DOI":"10.1038\/srep31329","type":"journal-article","created":{"date-parts":[[2016,8,10]],"date-time":"2016-08-10T10:58:06Z","timestamp":1470826686000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multifunctional metal-polymer nanoagglomerates from single-pass aerosol self-assembly"],"prefix":"10.1038","volume":"6","author":[{"given":"Jeong Hoon","family":"Byeon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,8,10]]},"reference":[{"key":"BFsrep31329_CR1","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nmat2442","volume":"8","author":"AE Nel","year":"2009","unstructured":"Nel, A. 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