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Herein, long-range dsDNA polymers could form based on the hybridization chain reaction (HCR) of two hairpin probes (H<jats:sub>1<\/jats:sub> and H<jats:sub>2<\/jats:sub>) by using miRNA-21 as an initiator. (+)AuNPs could adsorb onto the negatively charged surface of such long-range dsDNA polymers based on the electrostatic adsorption, which directly resulted in the precipitation of (+)AuNPs and the decrease of (+)AuNPs absorption spectra. Under optimal conditions, miRNA-21 detection could be realized in the range of 20\u2009pM-10\u2009nM with a detection limit of 6.8\u2009pM. In addition, (+)AuNPs used here are much more stable than commonly used negatively charged gold nanoparticles ((\u2212)AuNPs) in mixed solution that contained salt, protein or other metal ions. 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