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The pyrimidine glycosides vicine and convicine are precursors of the aglycones divicine and isouramil, the main factors of favism, a genetic condition which may lead to severe hemolysis after faba bean ingestion. The reduction of vicine and convicine has been targeted in several studies but little is known about their degradation. In this study, the hydrolysis kinetics of vicine and convicine and their derivatives during fermentation with <jats:italic>L. plantarum<\/jats:italic> DPPMAB24W was investigated. In particular, a specific HPLC method coupled to ESI-MS and MS\/MS analysis, including the evaluation procedure of the results, was set up as the analytical approach to monitor the compounds. The degradation of the pyrimidine glycosides in the fermented flour was complete after 48\u2009h of incubation and the aglycone derivatives could not be detected in any of the samples. The toxicity of the fermented faba bean was established through <jats:italic>ex-vivo<\/jats:italic> assays on human blood, confirming the experimental findings. Results indicate that mild and cost effective bioprocessing techniques can be applied to detoxify faba bean also for industrial applications.<\/jats:p>","DOI":"10.1038\/srep32452","type":"journal-article","created":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T16:01:46Z","timestamp":1472659306000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":107,"title":["Degradation of vicine, convicine and their aglycones during fermentation of faba bean flour"],"prefix":"10.1038","volume":"6","author":[{"given":"Carlo Giuseppe","family":"Rizzello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilario","family":"Losito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Facchini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kati","family":"Katina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Palmisano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Gobbetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana","family":"Coda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,8,31]]},"reference":[{"key":"BFsrep32452_CR1","doi-asserted-by":"publisher","first-page":"6634","DOI":"10.5897\/AJAR12.1003","volume":"8","author":"AK Singh","year":"2013","unstructured":"Singh, A. 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