{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:49:11Z","timestamp":1791434951525,"version":"4.3.3"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T00:00:00Z","timestamp":1472774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T00:00:00Z","timestamp":1472774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Acute and chronic tendinopathies remain clinically challenging and tendons are predisposed to degeneration or injury with age. Despite the high prevalence of tendon disease in the elderly, our current understanding of the mechanisms underlying the age-dependent deterioration of tendon function remains very limited. Here, we show that Secreted protein acidic and rich in cysteine (Sparc) expression significantly decreases in healthy-aged mouse Achilles tendons. Loss of Sparc results in tendon collagen fibrillogenesis defects and Sparc\u2212\/\u2212 tendons are less able to withstand force in comparison with their respective wild type counterparts. On the cellular level, Sparc-null and healthy-aged tendon-derived cells exhibited a more contracted phenotype and an altered actin cytoskeleton. Additionally, an elevated expression of the adipogenic marker genes PPAR\u03b3 and Cebp\u03b1 with a concomitant increase in lipid deposits in aged and Sparc\u2212\/\u2212 tendons was observed. In summary, we propose that Sparc levels in tendons are critical for proper collagen fibril maturation and its age-related decrease, together with a change in ECM properties favors lipid accretion in tendons.<\/jats:p>","DOI":"10.1038\/srep32635","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T05:00:04Z","timestamp":1472792404000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing"],"prefix":"10.1038","volume":"6","author":[{"given":"Renate","family":"Gehwolf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lehner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy D.","family":"Bradshaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Scharler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna A.","family":"Niestrawska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard A.","family":"Holzapfel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Christian","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Tempfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Traweger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,2]]},"reference":[{"key":"BFsrep32635_CR1","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1016\/j.injury.2008.06.021","volume":"39","author":"RA Clayton","year":"2008","unstructured":"Clayton, R. A. & Court-Brown, C. M. The epidemiology of musculoskeletal tendinous and ligamentous injuries. Injury 39, 1338\u20131344, 10.1016\/j.injury.2008.06.021 (2008).","journal-title":"Injury"},{"key":"BFsrep32635_CR2","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1177\/1938640009355191","volume":"3","author":"GW Hess","year":"2010","unstructured":"Hess, G. W. Achilles tendon rupture: a review of etiology, population, anatomy, risk factors, and injury prevention. Foot & ankle specialist 3, 29\u201332, 10.1177\/1938640009355191 (2010).","journal-title":"Foot & ankle specialist"},{"key":"BFsrep32635_CR3","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1002\/jor.22806","volume":"33","author":"S Thomopoulos","year":"2015","unstructured":"Thomopoulos, S., Parks, W. C., Rifkin, D. B. & Derwin, K. A. Mechanisms of tendon injury and repair. J Orthop Res 33, 832\u2013839, 10.1002\/jor.22806 (2015).","journal-title":"J Orthop Res"},{"key":"BFsrep32635_CR4","doi-asserted-by":"publisher","first-page":"170","DOI":"10.3109\/17453679709004002","volume":"68","author":"T Movin","year":"1997","unstructured":"Movin, T., Gad, A., Reinholt, F. P. & Rolf, C. Tendon pathology in long-standing achillodynia. Biopsy findings in 40 patients. Acta orthopaedica Scandinavica 68, 170\u2013175 (1997).","journal-title":"Acta orthopaedica Scandinavica"},{"key":"BFsrep32635_CR5","doi-asserted-by":"publisher","first-page":"746","DOI":"10.1136\/annrheumdis-2011-200391","volume":"71","author":"AN Corps","year":"2012","unstructured":"Corps, A. N. et al. Changes in matrix protein biochemistry and the expression of mRNA encoding matrix proteins and metalloproteinases in posterior tibialis tendinopathy. Annals of the rheumatic diseases 71, 746\u2013752, 10.1136\/annrheumdis-2011-200391 (2012).","journal-title":"Annals of the rheumatic diseases"},{"key":"BFsrep32635_CR6","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1093\/rheumatology\/40.2.229","volume":"40","author":"GP Riley","year":"2001","unstructured":"Riley, G. P., Goddard, M. J. & Hazleman, B. L. Histopathological assessment and pathological significance of matrix degeneration in supraspinatus tendons. Rheumatology 40, 229\u2013230 (2001).","journal-title":"Rheumatology"},{"key":"BFsrep32635_CR7","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1177\/039463200702001s12","volume":"20","author":"V Perrotti","year":"2007","unstructured":"Perrotti, V. et al. Vascular endothelial growth factor expression (VEGF) in salivary glands of diabetic rats. International journal of immunopathology and pharmacology 20, 55\u201360 (2007).","journal-title":"International journal of immunopathology and pharmacology"},{"key":"BFsrep32635_CR8","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1038\/nm.3651","volume":"20","author":"J Oh","year":"2014","unstructured":"Oh, J., Lee, Y. D. & Wagers, A. J. Stem cell aging: mechanisms, regulators and therapeutic opportunities. Nature medicine 20, 870\u2013880, 10.1038\/nm.3651 (2014).","journal-title":"Nature medicine"},{"key":"BFsrep32635_CR9","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1038\/nm1630","volume":"13","author":"Y Bi","year":"2007","unstructured":"Bi, Y. et al. Identification of tendon stem\/progenitor cells and the role of the extracellular matrix in their niche. Nature medicine 13, 1219\u20131227, 10.1038\/nm1630 (2007).","journal-title":"Nature medicine"},{"key":"BFsrep32635_CR10","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1089\/ten.TEA.2009.0529","volume":"16","author":"YF Rui","year":"2010","unstructured":"Rui, Y. F. et al. Isolation and characterization of multipotent rat tendon-derived stem cells. Tissue engineering. Part A 16, 1549\u20131558, 10.1089\/ten.TEA.2009.0529 (2010).","journal-title":"Tissue engineering. Part A"},{"key":"BFsrep32635_CR11","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/S0014-4827(03)00107-1","volume":"287","author":"R Salingcarnboriboon","year":"2003","unstructured":"Salingcarnboriboon, R. et al. Establishment of tendon-derived cell lines exhibiting pluripotent mesenchymal stem cell-like property. Exp Cell Res 287, 289\u2013300 (2003).","journal-title":"Exp Cell Res"},{"key":"BFsrep32635_CR12","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1111\/j.1582-4934.2009.00878.x","volume":"14","author":"F Deschaseaux","year":"2010","unstructured":"Deschaseaux, F., Pontikoglou, C. & Sensebe, L. Bone regeneration: the stem\/progenitor cells point of view. J Cell Mol Med 14, 103\u2013115, 10.1111\/j.1582-4934.2009.00878.x (2010).","journal-title":"J Cell Mol Med"},{"key":"BFsrep32635_CR13","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1111\/acel.12124","volume":"12","author":"J Kohler","year":"2013","unstructured":"Kohler, J. et al. Uncovering the cellular and molecular changes in tendon stem\/progenitor cells attributed to tendon aging and degeneration. Aging cell 12, 988\u2013999, 10.1111\/acel.12124 (2013).","journal-title":"Aging cell"},{"key":"BFsrep32635_CR14","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1111\/j.1474-9726.2010.00598.x","volume":"9","author":"Z Zhou","year":"2010","unstructured":"Zhou, Z. et al. Tendon-derived stem\/progenitor cell aging: defective self-renewal and altered fate. Aging cell 9, 911\u2013915, 10.1111\/j.1474-9726.2010.00598.x (2010).","journal-title":"Aging cell"},{"key":"BFsrep32635_CR15","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1007\/s12079-009-0062-6","volume":"3","author":"AD Bradshaw","year":"2009","unstructured":"Bradshaw, A. D. The role of SPARC in extracellular matrix assembly. J Cell Commun Signal 3, 239\u2013246, 10.1007\/s12079-009-0062-6 (2009).","journal-title":"J Cell Commun Signal"},{"key":"BFsrep32635_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.matbio.2014.07.005","volume":"37","author":"JE Murphy-Ullrich","year":"2014","unstructured":"Murphy-Ullrich, J. E. & Sage, E. H. Revisiting the matricellular concept. Matrix biology: journal of the International Society for Matrix Biology 37, 1\u201314, 10.1016\/j.matbio.2014.07.005 (2014).","journal-title":"Matrix biology: journal of the International Society for Matrix Biology"},{"key":"BFsrep32635_CR17","doi-asserted-by":"publisher","first-page":"454","DOI":"10.4161\/cbt.7.3.5455","volume":"7","author":"A Han","year":"2008","unstructured":"Han, A., Tong, C., Hu, D., Bi, X. & Yang, W. A direct protein-protein interaction is involved in the suppression of beta-catenin transcription by retinoid X receptor alpha in colorectal cancer cells. Cancer biology & therapy 7, 454\u2013459 (2008).","journal-title":"Cancer biology & therapy"},{"key":"BFsrep32635_CR18","unstructured":"Kannus, P., Paavola, M. & J\u00f3zsa, L. Aging and degeneration of tendons (Springer, 2005)."},{"key":"BFsrep32635_CR19","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","volume":"4","author":"W Huang da","year":"2009","unstructured":"Huang da, W., Sherman, B. T. & Lempicki, R. A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature protocols 4, 44\u201357, 10.1038\/nprot.2008.211 (2009).","journal-title":"Nature protocols"},{"key":"BFsrep32635_CR20","doi-asserted-by":"publisher","first-page":"10538","DOI":"10.1073\/pnas.1000525107","volume":"107","author":"Y Ito","year":"2010","unstructured":"Ito, Y. et al. The Mohawk homeobox gene is a critical regulator of tendon differentiation. Proc Natl Acad Sci USA 107, 10538\u201310542, 10.1073\/pnas.1000525107 (2010).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep32635_CR21","doi-asserted-by":"publisher","first-page":"17665","DOI":"10.1074\/jbc.M610113200","volume":"282","author":"V Lejard","year":"2007","unstructured":"Lejard, V. et al. Scleraxis and NFATc regulate the expression of the pro-alpha1(I) collagen gene in tendon fibroblasts. The Journal of biological chemistry 282, 17665\u201317675, 10.1074\/jbc.M610113200 (2007).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep32635_CR22","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/s13075-015-0544-2","volume":"17","author":"MJ Peffers","year":"2015","unstructured":"Peffers, M. J. et al. Transcriptome analysis of ageing in uninjured human Achilles tendon. Arthritis research & therapy 17, 33, 10.1186\/s13075-015-0544-2 (2015).","journal-title":"Arthritis research & therapy"},{"key":"BFsrep32635_CR23","doi-asserted-by":"publisher","first-page":"2203","DOI":"10.1007\/s11357-013-9514-2","volume":"35","author":"TY Kostrominova","year":"2013","unstructured":"Kostrominova, T. Y. & Brooks, S. V. Age-related changes in structure and extracellular matrix protein expression levels in rat tendons. Age (Dordr) 35, 2203\u20132214, 10.1007\/s11357-013-9514-2 (2013).","journal-title":"Age (Dordr)"},{"key":"BFsrep32635_CR24","doi-asserted-by":"publisher","first-page":"6045","DOI":"10.1073\/pnas.1030790100","volume":"100","author":"AD Bradshaw","year":"2003","unstructured":"Bradshaw, A. D., Graves, D. C., Motamed, K. & Sage, E. H. SPARC-null mice exhibit increased adiposity without significant differences in overall body weight. Proc Natl Acad Sci USA 100, 6045\u20136050, 10.1073\/pnas.1030790100 (2003).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep32635_CR25","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1002\/term.508","volume":"7","author":"NJ Turner","year":"2013","unstructured":"Turner, N. J., Pezzone, M. A., Brown, B. N. & Badylak, S. F. Quantitative multispectral imaging of Herovici\u2019s polychrome for the assessment of collagen content and tissue remodelling. Journal of tissue engineering and regenerative medicine 7, 139\u2013148, 10.1002\/term.508 (2013).","journal-title":"Journal of tissue engineering and regenerative medicine"},{"key":"BFsrep32635_CR26","first-page":"1183","volume":"35","author":"M Levame","year":"1987","unstructured":"Levame, M. & Meyer, F. [Herovici\u2019s picropolychromium. Application to the identification of type I and III collagens]. Pathologie-biologie 35, 1183\u20131188 (1987).","journal-title":"Pathologie-biologie"},{"key":"BFsrep32635_CR27","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1002\/term.13","volume":"1","author":"X Yang","year":"2007","unstructured":"Yang, X. et al. Multilineage potential of STRO-1+ rat dental pulp cells in vitro. Journal of tissue engineering and regenerative medicine 1, 128\u2013135, 10.1002\/term.13 (2007).","journal-title":"Journal of tissue engineering and regenerative medicine"},{"key":"BFsrep32635_CR28","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.1091\/mbc.10.5.1569","volume":"10","author":"AD Bradshaw","year":"1999","unstructured":"Bradshaw, A. D., Francki, A., Motamed, K., Howe, C. & Sage, E. H. Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation. Mol Biol Cell 10, 1569\u20131579 (1999).","journal-title":"Mol Biol Cell"},{"key":"BFsrep32635_CR29","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1164\/rccm.201306-1043OC","volume":"189","author":"ML Bustos","year":"2014","unstructured":"Bustos, M. L. et al. Aging Mesenchymal Stem Cells Fail to Protect Because of Impaired Migration and Antiinflammatory Response. American Journal of Respiratory and Critical Care Medicine 189, 787\u2013798, 10.1164\/rccm.201306-1043OC (2014).","journal-title":"American Journal of Respiratory and Critical Care Medicine"},{"key":"BFsrep32635_CR30","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1074\/jbc.M808285200","volume":"284","author":"J Nie","year":"2009","unstructured":"Nie, J. & Sage, E. H. SPARC inhibits adipogenesis by its enhancement of beta-catenin signaling. The Journal of biological chemistry 284, 1279\u20131290, 10.1074\/jbc.M808285200 (2009).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep32635_CR31","doi-asserted-by":"publisher","first-page":"3893","DOI":"10.1242\/jcs.00699","volume":"116","author":"N Kawaguchi","year":"2003","unstructured":"Kawaguchi, N. et al. ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating beta1 integrin function. J Cell Sci 116, 3893\u20133904, 10.1242\/jcs.00699 (2003).","journal-title":"J Cell Sci"},{"key":"BFsrep32635_CR32","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/S0074-7696(08)61539-9","volume":"150","author":"M Opas","year":"1994","unstructured":"Opas, M. Substratum mechanics and cell differentiation. Int Rev Cytol 150, 119\u2013137 (1994).","journal-title":"Int Rev Cytol"},{"key":"BFsrep32635_CR33","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1016\/0092-8674(83)90098-3","volume":"35","author":"BM Spiegelman","year":"1983","unstructured":"Spiegelman, B. M. & Ginty, C. A. Fibronectin modulation of cell shape and lipogenic gene expression in 3T3-adipocytes. Cell 35, 657\u2013666 (1983).","journal-title":"Cell"},{"key":"BFsrep32635_CR34","doi-asserted-by":"publisher","first-page":"45020","DOI":"10.1074\/jbc.M407050200","volume":"279","author":"J Liu","year":"2004","unstructured":"Liu, J. & Farmer, S. R. Regulating the balance between peroxisome proliferator-activated receptor gamma and beta-catenin signaling during adipogenesis. A glycogen synthase kinase 3beta phosphorylation-defective mutant of beta-catenin inhibits expression of a subset of adipogenic genes. The Journal of biological chemistry 279, 45020\u201345027, 10.1074\/jbc.M407050200 (2004).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep32635_CR35","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.fcl.2005.01.013","volume":"10","author":"TA Jarvinen","year":"2005","unstructured":"Jarvinen, T. A., Kannus, P., Maffulli, N. & Khan, K. M. Achilles tendon disorders: etiology and epidemiology. Foot and ankle clinics 10, 255\u2013266, 10.1016\/j.fcl.2005.01.013 (2005).","journal-title":"Foot and ankle clinics"},{"key":"BFsrep32635_CR36","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1161\/CIRCRESAHA.107.158147","volume":"102","author":"S Ryzhov","year":"2008","unstructured":"Ryzhov, S. et al. Adenosine receptor-mediated adhesion of endothelial progenitors to cardiac microvascular endothelial cells. Circulation research 102, 356\u2013363, 10.1161\/CIRCRESAHA.107.158147 (2008).","journal-title":"Circulation research"},{"key":"BFsrep32635_CR37","doi-asserted-by":"publisher","first-page":"12967","DOI":"10.1523\/JNEUROSCI.4061-07.2007","volume":"27","author":"DR Benavides","year":"2007","unstructured":"Benavides, D. R. et al. Cdk5 modulates cocaine reward, motivation, and striatal neuron excitability. The Journal of neuroscience: the official journal of the Society for Neuroscience 27, 12967\u201312976, 10.1523\/JNEUROSCI.4061-07.2007 (2007).","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"key":"BFsrep32635_CR38","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/S1058-2746(99)90148-9","volume":"8","author":"S Tempelhof","year":"1999","unstructured":"Tempelhof, S., Rupp, S. & Seil, R. Age-related prevalence of rotator cuff tears in asymptomatic shoulders. Journal of shoulder and elbow surgery\/American Shoulder and Elbow Surgeons \u2026 [et al.] 8, 296\u2013299 (1999).","journal-title":"Journal of shoulder and elbow surgery\/American Shoulder and Elbow Surgeons \u2026 [et al.]"},{"key":"BFsrep32635_CR39","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1038\/sj.cdd.4402275","volume":"15","author":"R Tufi","year":"2008","unstructured":"Tufi, R. et al. Reduction of endoplasmic reticulum Ca2+ levels favors plasma membrane surface exposure of calreticulin. Cell death and differentiation 15, 274\u2013282, 10.1038\/sj.cdd.4402275 (2008).","journal-title":"Cell death and differentiation"},{"key":"BFsrep32635_CR40","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.2106\/00004623-199173100-00009","volume":"73","author":"P Kannus","year":"1991","unstructured":"Kannus, P. & Jozsa, L. Histopathological changes preceding spontaneous rupture of a tendon. A controlled study of 891 patients. J Bone Joint Surg Am 73, 1507\u20131525 (1991).","journal-title":"J Bone Joint Surg Am"},{"key":"BFsrep32635_CR41","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1152\/japplphysiol.00460.2011","volume":"111","author":"LK Wood","year":"2011","unstructured":"Wood, L. K., Arruda, E. M. & Brooks, S. V. Regional stiffening with aging in tibialis anterior tendons of mice occurs independent of changes in collagen fibril morphology. Journal of applied physiology 111, 999\u20131006, 10.1152\/japplphysiol.00460.2011 (2011).","journal-title":"Journal of applied physiology"},{"key":"BFsrep32635_CR42","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.ijbiomac.2007.10.012","volume":"42","author":"A Coi","year":"2008","unstructured":"Coi, A. et al. Structural perturbation of alphaB-crystallin by zinc and temperature related to its chaperone-like activity. International journal of biological macromolecules 42, 229\u2013234, 10.1016\/j.ijbiomac.2007.10.012 (2008).","journal-title":"International journal of biological macromolecules"},{"key":"BFsrep32635_CR43","doi-asserted-by":"publisher","first-page":"021011","DOI":"10.1115\/1.2898732","volume":"130","author":"KL Goh","year":"2008","unstructured":"Goh, K. L. et al. Ageing changes in the tensile properties of tendons: influence of collagen fibril volume fraction. Journal of biomechanical engineering 130, 021011, 10.1115\/1.2898732 (2008).","journal-title":"Journal of biomechanical engineering"},{"key":"BFsrep32635_CR44","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.matbio.2012.11.005","volume":"32","author":"AA Dunkman","year":"2013","unstructured":"Dunkman, A. A. et al. Decorin expression is important for age-related changes in tendon structure and mechanical properties. Matrix biology: journal of the International Society for Matrix Biology 32, 3\u201313, 10.1016\/j.matbio.2012.11.005 (2013).","journal-title":"Matrix biology: journal of the International Society for Matrix Biology"},{"key":"BFsrep32635_CR45","doi-asserted-by":"crossref","unstructured":"Kalson, N. S. et al. A structure-based extracellular matrix expansion mechanism of fibrous tissue growth. eLife 4, 10.7554\/eLife.05958 (2015).","DOI":"10.7554\/eLife.05958"},{"key":"BFsrep32635_CR46","doi-asserted-by":"crossref","first-page":"22265","DOI":"10.1016\/S0021-9258(18)41664-X","volume":"267","author":"AM Romanic","year":"1992","unstructured":"Romanic, A. M., Adachi, E., Hojima, Y., Engel, J. & Prockop, D. J. Polymerization of pNcollagen I and copolymerization of pNcollagen I with collagen I. A kinetic, thermodynamic, and morphologic study. The Journal of biological chemistry 267, 22265\u201322271 (1992).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep32635_CR47","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1016\/j.jbiomech.2003.11.005","volume":"37","author":"TW Lin","year":"2004","unstructured":"Lin, T. W., Cardenas, L. & Soslowsky, L. J. Biomechanics of tendon injury and repair. Journal of biomechanics 37, 865\u2013877, 10.1016\/j.jbiomech.2003.11.005 (2004).","journal-title":"Journal of biomechanics"},{"key":"BFsrep32635_CR48","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1097\/FJC.0b013e31814b91cb","volume":"50","author":"S Straino","year":"2007","unstructured":"Straino, S. et al. Protective effects of parecoxib, a cyclo-oxygenase-2 inhibitor, in postinfarction remodeling in the rat. Journal of cardiovascular pharmacology 50, 571\u2013577, 10.1097\/FJC.0b013e31814b91cb (2007).","journal-title":"Journal of cardiovascular pharmacology"},{"key":"BFsrep32635_CR49","doi-asserted-by":"publisher","first-page":"1860","DOI":"10.1093\/emboj\/17.7.1860","volume":"17","author":"DT Gilmour","year":"1998","unstructured":"Gilmour, D. T. et al. Mice deficient for the secreted glycoprotein SPARC\/osteonectin\/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens. The EMBO journal 17, 1860\u20131870, 10.1093\/emboj\/17.7.1860 (1998).","journal-title":"The EMBO journal"},{"key":"BFsrep32635_CR50","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1172\/jci7039","volume":"105","author":"AM Delany","year":"2000","unstructured":"Delany, A. M. et al. Osteopenia and decreased bone formation in osteonectin-deficient mice. The Journal of clinical investigation 105, 915\u2013923, 10.1172\/jci7039 (2000).","journal-title":"The Journal of clinical investigation"},{"key":"BFsrep32635_CR51","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.jse.2013.04.011","volume":"23","author":"JP Gumucio","year":"2014","unstructured":"Gumucio, J. P. et al. Aging-associated exacerbation in fatty degeneration and infiltration after rotator cuff tear. Journal of shoulder and elbow surgery\/American Shoulder and Elbow Surgeons \u2026 [et al.] 23, 99\u2013108, 10.1016\/j.jse.2013.04.011 (2014).","journal-title":"Journal of shoulder and elbow surgery\/American Shoulder and Elbow Surgeons \u2026 [et al.]"},{"key":"BFsrep32635_CR52","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/S1534-5807(04)00075-9","volume":"6","author":"R McBeath","year":"2004","unstructured":"McBeath, R., Pirone, D. M., Nelson, C. M., Bhadriraju, K. & Chen, C. S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Developmental cell 6, 483\u2013495 (2004).","journal-title":"Developmental cell"},{"key":"BFsrep32635_CR53","first-page":"2674","volume":"39","author":"K Norose","year":"1998","unstructured":"Norose, K. et al. SPARC deficiency leads to early-onset cataractogenesis. Investigative ophthalmology & visual science 39, 2674\u20132680 (1998).","journal-title":"Investigative ophthalmology & visual science"},{"key":"BFsrep32635_CR54","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","volume":"15","author":"UK Laemmli","year":"1970","unstructured":"Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15, 680\u2013685 (1970).","journal-title":"Nature"},{"key":"BFsrep32635_CR55","doi-asserted-by":"publisher","first-page":"2520","DOI":"10.1002\/dvdy.22760","volume":"240","author":"NS Kalson","year":"2011","unstructured":"Kalson, N. S. et al. Slow stretching that mimics embryonic growth rate stimulates structural and mechanical development of tendon-like tissue in vitro. Developmental dynamics: an official publication of the American Association of Anatomists 240, 2520\u20132528, 10.1002\/dvdy.22760 (2011).","journal-title":"Developmental dynamics: an official publication of the American Association of Anatomists"},{"key":"BFsrep32635_CR56","doi-asserted-by":"publisher","first-page":"1275","DOI":"10.1098\/rsif.2011.0727","volume":"9","author":"AJ Schriefl","year":"2012","unstructured":"Schriefl, A. J., Zeindlinger, G., Pierce, D. M., Regitnig, P. & Holzapfel, G. A. Determination of the layer-specific distributed collagen fibre orientations in human thoracic and abdominal aortas and common iliac arteries. Journal of the Royal Society, Interface\/the Royal Society 9, 1275\u20131286, 10.1098\/rsif.2011.0727 (2012).","journal-title":"Journal of the Royal Society, Interface\/the Royal Society"},{"key":"BFsrep32635_CR57","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1002\/dvdy.23896","volume":"242","author":"A Herchenhan","year":"2013","unstructured":"Herchenhan, A., Bayer, M. L., Svensson, R. B., Magnusson, S. P. & Kjaer, M. In vitro tendon tissue development from human fibroblasts demonstrates collagen fibril diameter growth associated with a rise in mechanical strength. Dev Dyn 242, 2\u20138, 10.1002\/dvdy.23896 (2013).","journal-title":"Dev Dyn"},{"key":"BFsrep32635_CR58","doi-asserted-by":"publisher","first-page":"4889","DOI":"10.1016\/j.biomaterials.2010.02.062","volume":"31","author":"ML Bayer","year":"2010","unstructured":"Bayer, M. L. et al. The initiation of embryonic-like collagen fibrillogenesis by adult human tendon fibroblasts when cultured under tension. Biomaterials 31, 4889\u20134897, 10.1016\/j.biomaterials.2010.02.062 (2010).","journal-title":"Biomaterials"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep32635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep32635","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep32635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T18:42:06Z","timestamp":1672857726000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep32635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,2]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,16]]}},"alternative-id":["BFsrep32635"],"URL":"https:\/\/doi.org\/10.1038\/srep32635","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,2]]},"assertion":[{"value":"18 May 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 August 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 September 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"32635"}}