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In these diseases, the role of AMPK, a sensor and regulator of energy metabolism, is of biological and clinical importance. Since AMPK\u2019s main catalytic subunit \u03b1 has two isoforms, it is unclear whether functional differences between them exist in the skeletal system. The current study overexpressed AMPK\u03b11 and \u03b12 in MC3T3-E1 cells, primary osteoblasts and mouse BMSCs by lentiviral transduction. Cells overexpressing AMPK\u03b12 showed higher osteogenesis potential than AMPK\u03b11, wherein androgen receptor (AR) and osteoactivin played important roles. RANKL and M-CSF were secreted at lower levels from cells overexpressing \u03b12 than \u03b11, resulting in decreased osteoblast-associated osteoclastogenesis. Adipogenesis was inhibited to a greater degree in 3T3-L1 cells overexpressing \u03b12 than \u03b11, which was modulated by AR. An abnormal downregulation of AMPK\u03b12 was observed in human BMSCs exhibiting the fibrous dysplasia (FD) phenotype. Overexpression of AMPK\u03b12 in these cells rescued the defect in osteogenesis, suggesting that AMPK\u03b12 plays a role in FD pathogenesis. These findings highlight functional differences between AMPK\u03b11 and \u03b12, and provide a basis for investigating the molecular mechanisms of diseases associated with impaired functioning of the skeletal system.<\/jats:p>","DOI":"10.1038\/srep32771","type":"journal-article","created":{"date-parts":[[2016,9,7]],"date-time":"2016-09-07T10:13:40Z","timestamp":1473243220000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Functional differences between AMPK \u03b11 and \u03b12 subunits in osteogenesis, osteoblast-associated induction of osteoclastogenesis, and adipogenesis"],"prefix":"10.1038","volume":"6","author":[{"given":"Yu-gang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu-guo","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-rong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-ming","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-ting","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,7]]},"reference":[{"key":"BFsrep32771_CR1","doi-asserted-by":"crossref","first-page":"12187","DOI":"10.1074\/jbc.M112.430389","volume":"288","author":"H Kang","year":"2013","unstructured":"Kang, H., Viollet, B. & Wu, D. 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