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Of these strains, <jats:italic>P. aeruginosa<\/jats:italic> P378 and <jats:italic>K. pneumoniae<\/jats:italic> 1220 harbor the IMP-4-encoding plasmids pP378-IMP and p1220-IMP, respectively, whose complete nucleotide sequences are determined to be genetically closely related to the IncN1-type plasmid pIMP-HZ1. pP378-IMP\/p1220-IMP-like plasmids are hinted to be present in all the other <jats:italic>bla<\/jats:italic><jats:sub>IMP-4<\/jats:sub>-carrying strains, indicating the dissemination of pIMP-HZ1-related plasmids among <jats:italic>K. pneumoniae<\/jats:italic> or <jats:italic>P. aeruginosa<\/jats:italic> of different genotypes in this hospital. pP378-IMP carries two distinct accessory resistance regions, a <jats:italic>bla<\/jats:italic><jats:sub>IMP-4<\/jats:sub>-carrying class 1 integron In<jats:italic>823b<\/jats:italic>, and a truncated Tn<jats:italic>3<\/jats:italic>-family unit transposon \u0394Tn<jats:italic>6292<\/jats:italic>-3\u2032 harboring the quinolone resistance gene <jats:italic>qnrS1<\/jats:italic>. Massive fragmentation and rearrangement of these accessory genetic contents occur among p1220-IMP and IMP-HZ1 relative to pP378-IMP<jats:italic>. bla<\/jats:italic><jats:sub>IMP-4<\/jats:sub> is also present in the In<jats:italic>823b<\/jats:italic> remnants from p1220-IMP and IMP-HZ1, while <jats:italic>qnrS1<\/jats:italic> is located in a Tn<jats:italic>6292<\/jats:italic>-derive fragment from pIMP-HZ1 but not found in p1220-IMP. pP378-IMP represents the first fully sequenced IncN-type plasmid from <jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/srep33419","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T10:05:58Z","timestamp":1474279558000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Dissemination of IMP-4-encoding pIMP-HZ1-related plasmids among Klebsiella pneumoniae and Pseudomonas aeruginosa in a Chinese teaching hospital"],"prefix":"10.1038","volume":"6","author":[{"given":"Wei","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongsheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Defu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigang","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyuan","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,19]]},"reference":[{"key":"BFsrep33419_CR1","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1128\/AAC.04759-14","volume":"59","author":"S Kayama","year":"2015","unstructured":"Kayama, S. et al. 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