{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:59:37Z","timestamp":1790495977341,"version":"4.1.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T00:00:00Z","timestamp":1473984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T00:00:00Z","timestamp":1473984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pancreatic beta cell (\u03b2) dysfunction is an outcome of malnutrition. We assessed the role of the amplifying pathway (AMP PATH) in \u03b2 cells in malnourished obese mice. C57Bl-6 mice were fed a control (C) or a low-protein diet (R). The groups were then fed a high-fat diet (CH and RH). AMP PATH contribution to insulin secretion was assessed upon incubating islets with diazoxide and KCl. CH and RH displayed increased glucose intolerance, insulin resistance and glucose-stimulated insulin secretion. Only RH showed a higher contribution of the AMP PATH. The mitochondrial membrane potential of RH was decreased, and ATP flux was unaltered. In RH islets, glutamate dehydrogenase (GDH) protein content and activity increased, and the AMP PATH contribution was reestablished when GDH was blunted. Thus, protein malnutrition induces mitochondrial dysfunction in \u03b2 cells, leading to an increased contribution of the AMP PATH to insulin secretion through the enhancement of GDH content and activity.<\/jats:p>","DOI":"10.1038\/srep33464","type":"journal-article","created":{"date-parts":[[2016,9,16]],"date-time":"2016-09-16T10:48:31Z","timestamp":1474022911000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice"],"prefix":"10.1038","volume":"6","author":[{"given":"Nayara Carvalho","family":"Leite","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via","family":"de Paula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Cristine","family":"Borck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Franciesco","family":"Vettorazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato Chaves Souto","family":"Branco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Lubaczeuski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Carlos","family":"Boschero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio Cesar","family":"Zoppi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everardo Magalh\u00e3es","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,16]]},"reference":[{"key":"BFsrep33464_CR1","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/1471-2458-9-88","volume":"9","author":"DP Guh","year":"2009","unstructured":"Guh, D. P. et al. The incidence of co-morbidities related to obesity and overweight: a systematic review and meta-analysis. BMC public health 9, 88, doi: 10.1186\/1471-2458-9-88 (2009).","journal-title":"BMC public health"},{"key":"BFsrep33464_CR2","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1038\/nature05482","volume":"444","author":"SE Kahn","year":"2006","unstructured":"Kahn, S. E., Hull, R. L. & Utzschneider, K. M. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444, 840\u2013846, doi: 10.1038\/nature05482 (2006).","journal-title":"Nature"},{"key":"BFsrep33464_CR3","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1530\/acta.0.0550278","volume":"55","author":"E Cerasi","year":"1967","unstructured":"Cerasi, E. & Luft, R. The plasma insulin response to glucose infusion in healthy subjects and in diabetes mellitus. Acta endocrinologica 55, 278\u2013304 (1967).","journal-title":"Acta endocrinologica"},{"key":"BFsrep33464_CR4","doi-asserted-by":"publisher","first-page":"S245","DOI":"10.2337\/diabetes.51.2007.S245","volume":"51","author":"N Porksen","year":"2002","unstructured":"Porksen, N. et al. Pulsatile insulin secretion: detection, regulation, and role in diabetes. Diabetes 51 Suppl 1, S245\u2013S254 (2002).","journal-title":"Diabetes"},{"key":"BFsrep33464_CR5","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1111\/j.2040-1124.2010.00094.x","volume":"2","author":"G Leibowitz","year":"2011","unstructured":"Leibowitz, G., Kaiser, N. & Cerasi, E. beta-Cell failure in type 2 diabetes. J Diabetes Investig 2, 82\u201391, doi: 10.1111\/j.2040-1124.2010.00094.x (2011).","journal-title":"J Diabetes Investig"},{"key":"BFsrep33464_CR6","doi-asserted-by":"publisher","first-page":"S27","DOI":"10.1016\/S0168-8227(11)70010-9","volume":"93","author":"JC Henquin","year":"2011","unstructured":"Henquin, J. C. The dual control of insulin secretion by glucose involves triggering and amplifying pathways in beta-cells. Diabetes research and clinical practice 93 Suppl 1, S27\u2013S31, doi: 10.1016\/S0168-8227(11)70010-9 (2011).","journal-title":"Diabetes research and clinical practice"},{"key":"BFsrep33464_CR7","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1007\/s00125-009-1314-y","volume":"52","author":"JC Henquin","year":"2009","unstructured":"Henquin, J. C. Regulation of insulin secretion: a matter of phase control and amplitude modulation. Diabetologia 52, 739\u2013751, doi: 10.1007\/s00125-009-1314-y (2009).","journal-title":"Diabetologia"},{"key":"BFsrep33464_CR8","doi-asserted-by":"publisher","first-page":"1747","DOI":"10.1074\/jbc.M609875200","volume":"282","author":"A Szollosi","year":"2007","unstructured":"Szollosi, A., Nenquin, M., Aguilar-Bryan, L., Bryan, J. & Henquin, J. C. Glucose stimulates Ca2+ influx and insulin secretion in 2-week-old beta-cells lacking ATP-sensitive K+ channels. J Biol Chem 282, 1747\u20131756, doi: 10.1074\/jbc.M609875200 (2007).","journal-title":"J Biol Chem"},{"key":"BFsrep33464_CR9","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/j.biocel.2005.12.006","volume":"38","author":"P Maechler","year":"2006","unstructured":"Maechler, P., Carobbio, S. & Rubi, B. In beta-cells, mitochondria integrate and generate metabolic signals controlling insulin secretion. The international journal of biochemistry & cell biology 38, 696\u2013709, doi: 10.1016\/j.biocel.2005.12.006 (2006).","journal-title":"The international journal of biochemistry & cell biology"},{"key":"BFsrep33464_CR10","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.mce.2013.06.019","volume":"379","author":"P Maechler","year":"2013","unstructured":"Maechler, P. Mitochondrial function and insulin secretion. Molecular and cellular endocrinology 379, 12\u201318, doi: 10.1016\/j.mce.2013.06.019 (2013).","journal-title":"Molecular and cellular endocrinology"},{"key":"BFsrep33464_CR11","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1530\/JOE-12-0408","volume":"216","author":"JC de Oliveira","year":"2013","unstructured":"de Oliveira, J. C. et al. Poor pubertal protein nutrition disturbs glucose-induced insulin secretion process in pancreatic islets and programs rats in adulthood to increase fat accumulation. J Endocrinol 216, 195\u2013206, doi: 10.1530\/JOE-12-0408 (2013).","journal-title":"J Endocrinol"},{"key":"BFsrep33464_CR12","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1017\/S0007114509993060","volume":"103","author":"CC Zoppi","year":"2010","unstructured":"Zoppi, C. C. et al. Insulin release, peripheral insulin resistance and muscle function in protein malnutrition: a role of tricarboxylic acid cycle anaplerosis. The British journal of nutrition 103, 1237\u20131250, doi: 10.1017\/S0007114509993060 (2010).","journal-title":"The British journal of nutrition"},{"key":"BFsrep33464_CR13","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1007\/s00726-010-0716-y","volume":"40","author":"CA de Oliveira","year":"2011","unstructured":"de Oliveira, C. A., Latorraca, M. Q., de Mello, M. A. & Carneiro, E. M. Mechanisms of insulin secretion in malnutrition: modulation by amino acids in rodent models. Amino acids 40, 1027\u20131034, doi: 10.1007\/s00726-010-0716-y (2011).","journal-title":"Amino acids"},{"key":"BFsrep33464_CR14","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0955-2863(98)00080-1","volume":"10","author":"MQ Latorraca","year":"1999","unstructured":"Latorraca, M. Q., Carneiro, E. M., Mello, M. A. & Boschero, A. C. Reduced insulin secretion in response to nutrients in islets from malnourished young rats is associated with a diminished calcium uptake. The Journal of nutritional biochemistry 10, 37\u201343 (1999).","journal-title":"The Journal of nutritional biochemistry"},{"key":"BFsrep33464_CR15","doi-asserted-by":"publisher","first-page":"149","DOI":"10.4239\/wjd.v2.i9.149","volume":"2","author":"B Reusens","year":"2011","unstructured":"Reusens, B., Theys, N. & Remacle, C. Alteration of mitochondrial function in adult rat offspring of malnourished dams. World journal of diabetes 2, 149\u2013157, doi: 10.4239\/wjd.v2.i9.149 (2011).","journal-title":"World journal of diabetes"},{"key":"BFsrep33464_CR16","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1007\/s13679-015-0170-y","volume":"4","author":"A Abdullah","year":"2015","unstructured":"Abdullah, A. The Double Burden of Undernutrition and Overnutrition in Developing Countries: an Update. Curr Obes Rep 4, 337\u2013349, doi: 10.1007\/s13679-015-0170-y (2015).","journal-title":"Curr Obes Rep"},{"key":"BFsrep33464_CR17","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1093\/ije\/dyt133","volume":"42","author":"CN Hales","year":"2013","unstructured":"Hales, C. N. & Barker, D. J. Type 2 (non-insulin-dependent) diabetes mellitus: the thrifty phenotype hypothesis. Int J Epidemiol 42, 1215\u20131222, doi: 10.1093\/ije\/dyt133 (2013).","journal-title":"Int J Epidemiol"},{"key":"BFsrep33464_CR18","doi-asserted-by":"publisher","first-page":"2123","DOI":"10.1007\/s00726-014-1763-6","volume":"46","author":"JF Vettorazzi","year":"2014","unstructured":"Vettorazzi, J. F. et al. Taurine supplementation increases K(ATP) channel protein content, improving Ca2+ handling and insulin secretion in islets from malnourished mice fed on a high-fat diet. Amino acids 46, 2123\u20132136, doi: 10.1007\/s00726-014-1763-6 (2014).","journal-title":"Amino acids"},{"key":"BFsrep33464_CR19","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1007\/s00125-010-1898-2","volume":"54","author":"B Valtat","year":"2011","unstructured":"Valtat, B. et al. Genetic evidence of the programming of beta cell mass and function by glucocorticoids in mice. Diabetologia 54, 350\u2013359, doi: 10.1007\/s00125-010-1898-2 (2011).","journal-title":"Diabetologia"},{"key":"BFsrep33464_CR20","doi-asserted-by":"publisher","first-page":"E592","DOI":"10.1152\/ajpendo.2001.281.3.E592","volume":"281","author":"B Blondeau","year":"2001","unstructured":"Blondeau, B., Lesage, J., Czernichow, P., Dupouy, J. P. & Breant, B. Glucocorticoids impair fetal beta-cell development in rats. American journal of physiology. Endocrinology and metabolism 281, E592\u2013E599 (2001).","journal-title":"American journal of physiology. Endocrinology and metabolism"},{"key":"BFsrep33464_CR21","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1006\/bbrc.1996.0439","volume":"220","author":"Y Tokuyama","year":"1996","unstructured":"Tokuyama, Y. et al. Rat inwardly rectifying potassium channel Kir6.2: cloning electrophysiological characterization, and decreased expression in pancreatic islets of male Zucker diabetic fatty rats. Biochemical and biophysical research communications 220, 532\u2013538, doi: 10.1006\/bbrc.1996.0439 (1996).","journal-title":"Biochemical and biophysical research communications"},{"key":"BFsrep33464_CR22","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.ceca.2011.11.006","volume":"51","author":"P Rorsman","year":"2012","unstructured":"Rorsman, P., Braun, M. & Zhang, Q. Regulation of calcium in pancreatic alpha- and beta-cells in health and disease. Cell calcium 51, 300\u2013308, doi: 10.1016\/j.ceca.2011.11.006 (2012).","journal-title":"Cell calcium"},{"key":"BFsrep33464_CR23","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/978-1-4614-6093-0_10","volume":"776","author":"RL Camargo","year":"2013","unstructured":"Camargo, R. L. et al. Effects of taurine supplementation upon food intake and central insulin signaling in malnourished mice fed on a high-fat diet. Advances in experimental medicine and biology 776, 93\u2013103, doi: 10.1007\/978-1-4614-6093-0_10 (2013).","journal-title":"Advances in experimental medicine and biology"},{"key":"BFsrep33464_CR24","doi-asserted-by":"publisher","first-page":"14768","DOI":"10.1074\/jbc.M701382200","volume":"282","author":"A Szollosi","year":"2007","unstructured":"Szollosi, A., Nenquin, M. & Henquin, J. C. Overnight culture unmasks glucose-induced insulin secretion in mouse islets lacking ATP-sensitive K+ channels by improving the triggering Ca2+ signal. The Journal of biological chemistry 282, 14768\u201314776, doi: 10.1074\/jbc.M701382200 (2007).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep33464_CR25","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/nrm2327","volume":"9","author":"DM Muoio","year":"2008","unstructured":"Muoio, D. M. & Newgard, C. B. Mechanisms of disease:Molecular and metabolic mechanisms of insulin resistance and beta-cell failure in type 2 diabetes. Nature reviews. Molecular cell biology 9, 193\u2013205, doi: 10.1038\/nrm2327 (2008).","journal-title":"Nature reviews. Molecular cell biology"},{"key":"BFsrep33464_CR26","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.mce.2015.01.033","volume":"404","author":"E Irles","year":"2015","unstructured":"Irles, E. et al. Enhanced glucose-induced intracellular signaling promotes insulin hypersecretion: Pancreatic beta-cell functional adaptations in a model of genetic obesity and prediabetes. Molecular and cellular endocrinology 404, 46\u201355, doi: 10.1016\/j.mce.2015.01.033 (2015).","journal-title":"Molecular and cellular endocrinology"},{"key":"BFsrep33464_CR27","doi-asserted-by":"publisher","first-page":"3515","DOI":"10.1210\/En.2013-1424","volume":"154","author":"A Gonzalez","year":"2013","unstructured":"Gonzalez, A. et al. Insulin Hypersecretion in Islets From Diet-Induced Hyperinsulinemic Obese Female Mice Is Associated With Several Functional Adaptations in Individual beta-Cells. Endocrinology 154, 3515\u20133524, doi: 10.1210\/En.2013-1424 (2013).","journal-title":"Endocrinology"},{"key":"BFsrep33464_CR28","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1016\/j.metabol.2010.12.010","volume":"60","author":"ML Bonfleur","year":"2011","unstructured":"Bonfleur, M. L. et al. Lower expression of PKAalpha impairs insulin secretion in islets isolated from low-density lipoprotein receptor (LDLR(-\/-)) knockout mice. Metabolism: clinical and experimental 60, 1158\u20131164, doi: 10.1016\/j.metabol.2010.12.010 (2011).","journal-title":"Metabolism: clinical and experimental"},{"key":"BFsrep33464_CR29","doi-asserted-by":"publisher","first-page":"4521","DOI":"10.1007\/s11033-013-2546-y","volume":"40","author":"RA Ribeiro","year":"2013","unstructured":"Ribeiro, R. A. et al. Impaired muscarinic type 3 (M3) receptor\/PKC and PKA pathways in islets from MSG-obese rats. Molecular biology reports 40, 4521\u20134528, doi: 10.1007\/s11033-013-2546-y (2013).","journal-title":"Molecular biology reports"},{"key":"BFsrep33464_CR30","doi-asserted-by":"publisher","first-page":"E207","DOI":"10.1152\/ajpendo.00426.2003","volume":"287","author":"MZ Khaldi","year":"2004","unstructured":"Khaldi, M. Z., Guiot, Y., Gilon, P., Henquin, J. C. & Jonas, J. C. Increased glucose sensitivity of both triggering and amplifying pathways of insulin secretion in rat islets cultured for 1 wk in high glucose. American journal of physiology. Endocrinology and metabolism 287, E207\u2013E217, doi: 10.1152\/ajpendo.00426.2003 (2004).","journal-title":"American journal of physiology. Endocrinology and metabolism"},{"key":"BFsrep33464_CR31","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1016\/j.jnutbio.2010.08.015","volume":"22","author":"N Theys","year":"2011","unstructured":"Theys, N., Ahn, M. T., Bouckenooghe, T., Reusens, B. & Remacle, C. Maternal malnutrition programs pancreatic islet mitochondrial dysfunction in the adult offspring. The Journal of nutritional biochemistry 22, 985\u2013994, doi: 10.1016\/j.jnutbio.2010.08.015 (2011).","journal-title":"The Journal of nutritional biochemistry"},{"key":"BFsrep33464_CR32","doi-asserted-by":"publisher","first-page":"39163","DOI":"10.1074\/jbc.M207157200","volume":"277","author":"YQ Liu","year":"2002","unstructured":"Liu, Y. Q., Jetton, T. L. & Leahy, J. L. beta-Cell adaptation to insulin resistance. Increased pyruvate carboxylase and malate-pyruvate shuttle activity in islets of nondiabetic Zucker fatty rats. The Journal of biological chemistry 277, 39163\u201339168, doi: 10.1074\/jbc.M207157200 (2002).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep33464_CR33","doi-asserted-by":"publisher","first-page":"e6110","DOI":"10.1371\/journal.pone.0006110","volume":"4","author":"N Theys","year":"2009","unstructured":"Theys, N., Clippe, A., Bouckenooghe, T., Reusens, B. & Remacle, C. Early low protein diet aggravates unbalance between antioxidant enzymes leading to islet dysfunction. PloS one 4, e6110, doi: 10.1371\/journal.pone.0006110 (2009).","journal-title":"PloS one"},{"key":"BFsrep33464_CR34","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1074\/jbc.M806295200","volume":"284","author":"S Carobbio","year":"2009","unstructured":"Carobbio, S. et al. Deletion of glutamate dehydrogenase in beta-cells abolishes part of the insulin secretory response not required for glucose homeostasis. The Journal of biological chemistry 284, 921\u2013929, doi: 10.1074\/jbc.M806295200 (2009).","journal-title":"The Journal of biological chemistry"},{"key":"BFsrep33464_CR35","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1007\/s00125-003-1306-2","volume":"47","author":"S Carobbio","year":"2004","unstructured":"Carobbio, S. et al. Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets. Diabetologia 47, 266\u2013276, doi: 10.1007\/s00125-003-1306-2 (2004).","journal-title":"Diabetologia"},{"key":"BFsrep33464_CR36","doi-asserted-by":"publisher","first-page":"667","DOI":"10.2337\/diabetes.49.4.667","volume":"49","author":"CA Stanley","year":"2000","unstructured":"Stanley, C. A. et al. Molecular basis and characterization of the hyperinsulinism\/hyperammonemia syndrome: predominance of mutations in exons 11 and 12 of the glutamate dehydrogenase gene. HI\/HA Contributing Investigators. Diabetes 49, 667\u2013673 (2000).","journal-title":"Diabetes"},{"key":"BFsrep33464_CR37","doi-asserted-by":"publisher","first-page":"3851","DOI":"10.1091\/mbc.E11-08-0676","volume":"23","author":"L Vetterli","year":"2012","unstructured":"Vetterli, L. et al. Delineation of glutamate pathways and secretory responses in pancreatic islets with beta-cell-specific abrogation of the glutamate dehydrogenase. Molecular biology of the cell 23, 3851\u20133862, doi: 10.1091\/mbc.E11-08-0676 (2012).","journal-title":"Molecular biology of the cell"},{"key":"BFsrep33464_CR38","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1089\/ars.2009.2531","volume":"12","author":"WI Sivitz","year":"2010","unstructured":"Sivitz, W. I. & Yorek, M. A. Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxidants & redox signaling 12, 537\u2013577, doi: 10.1089\/ars.2009.2531 (2010).","journal-title":"Antioxidants & redox signaling"},{"key":"BFsrep33464_CR39","doi-asserted-by":"publisher","first-page":"2792","DOI":"10.2337\/diabetes.50.12.2792","volume":"50","author":"A Bierhaus","year":"2001","unstructured":"Bierhaus, A. et al. Diabetes-associated sustained activation of the transcription factor nuclear factor-kappaB. Diabetes 50, 2792\u20132808 (2001).","journal-title":"Diabetes"},{"key":"BFsrep33464_CR40","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/j.metabol.2011.09.012","volume":"61","author":"PM da Silva","year":"2012","unstructured":"da Silva, P. M. et al. Decreased insulin secretion in islets from protein malnourished rats is associated with impaired glutamate dehydrogenase function: effect of leucine supplementation. Metabolism 61, 721\u2013732, doi: 10.1016\/j.metabol.2011.09.012 (2012).","journal-title":"Metabolism"},{"key":"BFsrep33464_CR41","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1016\/j.jnutbio.2014.12.010","volume":"26","author":"BR Lippo","year":"2015","unstructured":"Lippo, B. R. et al. Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets. J Nutr Biochem 26, 556\u2013562, doi: 10.1016\/j.jnutbio.2014.12.010 (2015).","journal-title":"J Nutr Biochem"},{"key":"BFsrep33464_CR42","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1677\/joe.0.0400527","volume":"40","author":"LL Bernardis","year":"1968","unstructured":"Bernardis, L. L. & Patterson, B. D. Correlation between \u2018Lee index\u2019 and carcass fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol 40, 527\u2013528 (1968).","journal-title":"J Endocrinol"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep33464.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep33464","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep33464.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T06:49:11Z","timestamp":1672901351000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep33464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,16]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,16]]}},"alternative-id":["BFsrep33464"],"URL":"https:\/\/doi.org\/10.1038\/srep33464","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,16]]},"assertion":[{"value":"22 September 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 August 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"33464"}}