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Earlier studies in the human malaria parasite <jats:italic>Plasmodium falciparum<\/jats:italic> have shown that pantothenate analogs interfere with pantothenate phosphorylation and block asexual blood stage development. Although two eukaryotic-type putative pantothenate kinase genes (<jats:italic>PanK1<\/jats:italic> and <jats:italic>PanK2<\/jats:italic>) have been identified in all malaria parasite species, their role in the development of <jats:italic>Plasmodium<\/jats:italic> life cycle stages remains unknown. Here we report on the genetic characterization of <jats:italic>PanK1<\/jats:italic> and <jats:italic>PanK2<\/jats:italic> in <jats:italic>P. yoelii<\/jats:italic>. We show that <jats:italic>P. yoelii<\/jats:italic> parasites lacking either <jats:italic>PanK1<\/jats:italic> or <jats:italic>PanK2<\/jats:italic> undergo normal asexual stages development and sexual stages differentiation, however they are severely deficient in ookinete, oocyst and sporozoite formation inside the mosquito vector. Quantitative transcriptional analyses in wild-type and knockout parasites demonstrate an important role for these genes in the regulation of expression of other CoA biosynthesis genes. Together, our data provide the first genetic evidence for the importance of the early steps of pantothenate utilization in the regulation of CoA biosynthesis and malaria parasite transmission to <jats:italic>Anopheles<\/jats:italic> mosquitoes.<\/jats:p>","DOI":"10.1038\/srep33518","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T09:20:07Z","timestamp":1474363207000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Genetic Characterization of Plasmodium Putative Pantothenate Kinase Genes Reveals Their Essential Role in Malaria Parasite Transmission to the Mosquito"],"prefix":"10.1038","volume":"6","author":[{"given":"Robert J.","family":"Hart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Cornillot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanah","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Molina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine S.","family":"Nation","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choukri","family":"Ben Mamoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed S. 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