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We sought to determine whether an extract of olive and grape seed standardized according to hydroxytyrosol (HT) and procyanidins (PCy) content, exerts preventive anti-osteoathritic effects. To this aim, we evaluated whether the HT\/PCy mix could (i) have <jats:italic>in vitro<\/jats:italic> anti-inflammatory and chondroprotective actions, (ii) exert anti-osteoarthritis effects in two post-traumatic animal models and (iii) retain its bioactivity after oral administration. Anti-inflammatory and chondroprotective actions of HT\/PCy were tested on primary cultured rabbit chondrocytes stimulated by interleukin-1 beta (IL-1\u03b2). The results showed that HT\/PCy exerts anti-inflammatory and chondroprotective actions <jats:italic>in vitro<\/jats:italic>. The preventive effect of HT\/PCy association was assessed in two animal models of post-traumatic OA in mice and rabbits. Diet supplementation with HT\/PCy significantly decreased the severity of post-traumatic osteoarthritis in two complementary mice and rabbit models. The bioavailability and bioactivity was evaluated following gavage with HT\/PCy in rabbits. Regular metabolites from HT\/PCy extract were found in sera from rabbits following oral intake. Finally, sera from rabbits force-fed with HT\/PCy conserved anti-IL-1\u03b2 effect, suggesting the bioactivity of this extract. To conclude, HT\/PCy extract may be of clinical significance for the preventive treatment of osteoarthritis.<\/jats:p>","DOI":"10.1038\/srep33527","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T10:21:52Z","timestamp":1474280512000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1\u03b2 activities before and after oral consumption"],"prefix":"10.1038","volume":"6","author":[{"given":"Elsa","family":"M\u00e9vel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Merceron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Vinatier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Krisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martial","family":"Masson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Lesoeur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Hivernaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Gauthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Abadie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffroy","family":"Nourissat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Houard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohann","family":"Wittrant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelly","family":"Urban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Guicheux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,19]]},"reference":[{"key":"BFsrep33527_CR1","doi-asserted-by":"crossref","first-page":"d1165","DOI":"10.1136\/bmj.d1165","volume":"342","author":"E Nuesch","year":"2011","unstructured":"Nuesch, E. et al. 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