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Epidemiological data indicate that MetS may increase the risks of overactive bladder (OAB) symptoms. However, it remains unknown whether MFE programs MetS-associated bladder dysfunction in adult offspring. Using Sprague-Dawley rats, we investigated the effects of MFE during pregnancy and lactation on developmental programming of MetS-associated bladder dysfunction. In addition, next generation sequencing technology was used to identify potential transcripts involved in the programmed bladder dysfunction in adult male offspring to MFE. We found that MFE programmed the MetS-associated OAB symptoms (i.e., an increase in micturition frequency and a shortened mean inter-contractile interval) in young adult male offspring, alongside significant alterations in bladder transcripts, including <jats:italic>Chrm2, Chrm3, P2rx1, Trpv4,<\/jats:italic> and <jats:italic>Vipr2<\/jats:italic> gene expression. At protein level, the expressions of M<jats:sub>2<\/jats:sub>-, M<jats:sub>3<\/jats:sub>-muscarinic and P2X<jats:sub>1<\/jats:sub> receptor proteins were upregulated in the MFE bladder. Functionally, the carbachol-induced detrusor contractility was reduced in the MFE offspring. These data suggest that alterations in the bladder transcripts and impairment of the bladder cholinergic pathways may underlie the pathophysiology of programmed bladder dysfunction in adult offspring to MFE.<\/jats:p>","DOI":"10.1038\/srep34669","type":"journal-article","created":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T09:23:28Z","timestamp":1475659408000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Maternal Fructose Exposure Programs Metabolic Syndrome-Associated Bladder Overactivity in Young Adult Offspring"],"prefix":"10.1038","volume":"6","author":[{"given":"Wei-Chia","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Lin","family":"Tain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay L. 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