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It may be used for food poisoning or bioterrorism, seriously endangering public health. In this study, a reliable method for the rapid detection of abrin in foods was developed, based on an up-converting phosphor technology-based lateral flow assay (abrin-UPT-LFA). Nine high-affinity monoclonal antibodies (mAbs) against abrin were prepared, and the optimum mAbs (mAb-6F4 and mAb-10E11) were selected for use in the assay in double-antibody-sandwich mode. The assay was confirmed to be specific for abrin, with a detection sensitivity of 0.1\u2009ng mL<jats:sup>\u22121<\/jats:sup> for standard abrin solutions. Good linearity was observed for abrin quantitation from 0.1 to 1000\u2009ng mL<jats:sup>\u22121<\/jats:sup> (<jats:italic>r<\/jats:italic>\u2009=\u20090.9983). During the analysis of various abrin-spiked food samples, the assay showed strong sample tolerance and a satisfactory limit of detection for abrin (0.5\u201310\u2009ng g<jats:sup>\u22121<\/jats:sup> for solid and powdered samples; 0.30\u20130.43\u2009ng mL<jats:sup>\u22121<\/jats:sup> for liquid samples). The analysis of suspected food samples, from sample treatment to result feed-back, could be completed by non-professionals within 20\u2009min. Therefore, the abrin-UPT-LFA is a rapid, sensitive, and reliable method for the on-site detection of abrin in foods.<\/jats:p>","DOI":"10.1038\/srep34926","type":"journal-article","created":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T10:07:29Z","timestamp":1475662049000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Rapid detection of abrin in foods with an up-converting phosphor technology-based lateral flow assay"],"prefix":"10.1038","volume":"6","author":[{"given":"Xiao","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongyun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinrui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfu","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,10,5]]},"reference":[{"key":"BFsrep34926_CR1","doi-asserted-by":"publisher","first-page":"e58304","DOI":"10.1371\/journal.pone.0058304","volume":"8","author":"S Gadadhar","year":"2013","unstructured":"Gadadhar, S. & Karande, A. 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